Kharlamov AV, Frolov AN, Zavyalov OA, Kosilov VI, Morgan GA

DOI: 10.33284/2658-3135-102-4-43

UDC 636.082.11:591.11

Acknowledgements:

Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0006)

 

The influence of polymorphism of growth differentiation factor 5 on elemental status of bulls

AnatolyV Kharlamov1, Alexey N Frolov1, Oleg A Zavyalov1, Vladimir I Kosilov2, Gary A Morgan3

1Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences(Orenburg, Russia)

2Orenburg State Agrarian University (Orenburg, Russia)

3VeterinaryManagementServicesLtd (Prince Edward Island, Canada)

Summary. The studies were performed on calves of the Kalmyk breed (n = 182), the age of selecting biosubstrates was 12-14 months, live weight was 343.7 ± 6.4 kg. At the first stage, blood samples were taken to determine the single nucleotide polymorphism of growth differentiation factor 5 (T586C in exon 1). DNA samples were isolated from whole blood using an IsoGeneLab reagent kit. The primers used in the study were synthesized in NPF Litech. For amplification of DNA, the MyCycler thermal cycler was used.

The frequency of occurrence of polymorphic groups in the GDF5 gene was established, it was according to homozygous alleles: T – 48.9%, C – 4.4%, heterozygous – 46.7%.

At the second stage of research, hair samples were taken from the upper part of withers of 8 animals from each identified group of animals. The elemental composition of biosubstrates was studied according to 25 indi-cators, using atomic emission and mass spectrometry (AES-ICP and MS-ICP).

The effect of polymorphism in the gene of growth differentiation factor 5 on the elemental status of bulls has been established. So, hair of bulls with CC genotype contained more macro- and essential elements: Ca – 38.4 and 35.8%, K – 169.1 and 113.1%, Na – 112.9 and 92.2 %, I – by 39.0 and 30.4%, Se – by 21.1 and 18.8%, with a decrease in toxic: As – by 37.5 and 2.3%, Al – by 61.2 and 50,8%, Pb – by 52.3 and 39.0% relative to animals with TT and TC genotypes, respectively.

It is concluded that further research is needed with a larger group of animals according to homozygous genotype C.

Key words: cattle, bulls, gene, GDF5, SNP, elemental status, toxic elements.

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Kharlamov Anatoly Vasilyevich, Dr. Sci. (Agr.), Professor, Head of Department of Technology for Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78, e-mail: vniims.or@mail.ru

Frolov Alexey Nikolaevich, Cand. Sci. (Agr.), Senior Researcher, Department of Technology for Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78, e-mail: forleh@mail.ru

Zavyalov Oleg Alexandrovich, Cand. Sci. (Agr.), Senior Researcher, Department of Technology of Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78, e-mail: oleg-zavyalov83@mail.ru

Kosilov Vladimir Ivanovich, Dr. Sci. (Agr.), Professor of Department of Production Technology and Processing of Livestock Products, Orenburg State Agrarian University, 460014, Orenburg, Russia, 18 Chelyuskintsev, St.

Morgan Gary A., Dr. Sci. (Vet.), Scientific Consultant, Veterinary Management Services Ltd, Prince Edward Island, Canada COB 1VO, тел.: +1(902)859184

Received: 11 December 2019; Accepted: 16 December 2019;Published: 31 December 2019

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