Vitaly A Ryazanov, Georgy I Levakhin, Galimzhan K Duskaev, Elena V Sheyda, Baer S Nurzhanov, Ivan S Miroshnikov

DOI: 10.33284/2658-3135-104-3-36

UDC 636.084.1:665.12

Acknowledgements:

Research was carried out according the plan of research scientific works on 2021-2023 yy. FSBSI FRC BST RAS (No 0761-2019-0005)

Changes in the taxonomic profile of microorganisms in ruminal fluid of cattle against

the background of fat-containing feed additives (in vitro study)

 Vitaly A Ryazanov, Georgy I Levakhin, Galimzhan K Duskaev, Elena V Sheyda, Baer S Nurzhanov, Ivan S Miroshnikov

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

 Abstract. The article presents the results of the in vitro study of ruminal fluid of young cattle under the influence of new fatty combinations, such as flaxseed oil and fish oil mixed with stearic acid on the taxonomic composition of microflora. The study found that the distribution of gram-negative and gram-positive bacteria for the phyla Firmicutes and Bacteroidetes was 1:2.8 in the group with linseed oil with added stearic acid, in the group with fish oil treated with stearic acid, the ratio for these phyla was 1:1.9 The archaeal domain of the total biomass was revealed in the amount of 0.08% (P≤0.05) and 0.09% (P≤0.05). The combination of fish oil and stearic acid in vitro revealed the Christensenellaceae family (3.8%).

Keywords: cattle, bulls, feeding, stearic acid, flaxseed oil, fish oil, rumen microbiome, methane.

References

  1. GOST 5791-81. Linseed oil for industrial use. Technical conditions. Intro. 01.07.1982. Moscow: Standartinform; 2011:5 p.
  2. GOST 6484-96. Technical stearic acid (stearin). Technical conditions. Intro. 01.01.1997. Moscow: Minsk: Interstate Council for Standardization, Metrology and Certification; 1997:12 p.
  3. GOST 8714-2014. Edible fat from fish and aquatic mammals. Technical conditions. Intro. 01.07.2015. Moscow: Standartinform; 2019:5 p.
  4. Elina EE. Biodiversity: Method. manual for bachelors of the training programme "Ecology and nature management". Orenburg: Express Printing Printing House; 2016:36 p.
  5. AbuGhazaleh A, Jacobson BN. Production of trans C18:1 and conjugated linoleic acid in continuous culture fermenters fed diets containing fish oil and sunflower oil with decreasing levels of forage. Animal. 2007;1(5):660-665. doi: 10.1017/S1751731107727489
  6. Atikah IN, Alimon AR, Yaakub H, Abdullah N, Jahromi MF, Ivan M, Samsudin AA. Profiling of rumen fermentation, microbial populatуion and digestibility in goats fed with dietary oils containing different fatty acids. BMC Vet Res. 2018;14(1):344. doi: 10.1186/s12917-018-1672-0
  7. Bayat AR, Ventto L, Kairenius P, Stefański T, Leskinen H, Tapio I, Negussie E, Vilkki J, Shingfield KJ. Dietary forage to concentrate ratio and sunflower oil supplement alter rumen fermentation, ruminal methane emissions, and nutrient utilization in lactating cows. Transl Anim Sci. 2017;1(3):277-286. doi: 10.2527/tas2017.0032
  8. Behan AA, Loh TC, Fakurazi S, Kaka U, Kaka A, Samsudin AA. Effects of supplementation of rumen protected fats on rumen ecology and digestibility of nutrients in sheep. Animals (Basel). 2019; 9(7):400. doi: 10.3390/ani9070400
  9. Benchaar C, Hassanat F, Martineau R, Gervais R. Linseed oil supplementation to dairy cows fed diets  based  on  red  clover  silage  or  corn  silage:  Effects  on  methane  production,  rumen  fermentation, nutrient digestibility, N balance, and milk production. J Dairy Sci. 2015;98(11):7993-8008. doi: 10.3168/jds.2015-9398.
  10. Cozzi G, Brscic M, Gottardo F. Main critical factors affecting the welfare of beef cattle and veal calves raised under intensive rearing systems in Italy: a review. Ital J Anim Sci. 2009;8(1):67-80. doi: 10.4081/ijas.2009.s1.67
  11. Debruyne S, Ruiz-González A, Artiles-Ortega E, Ampe B, Broeck VDW, Keyser ED, Vandaele L, Goossens K,  Fievez V. Supplementing goat kids with coconut medium chain fatty acids in early life influences growth and rumen papillae development until 4 months after supplementation but effects on in vitro methane emissions and the rumen microbiota are transient. J Anim Sci.2018;96(5):1978-1995. doi: 10.1093/jas/sky070
  12. Goodrich JK, Waters JL, Poole AC, Sutter JL, Koren O, Blekhman R, Beaumont M, Treuren WV, Knight R, Bell JT, Spector TD, Clark AG, Ley RE. Human genetics shape the gut microbiome. 2014;159(4):789-799. Cell. doi: 10.1016/j.cell.2014.09.053
  13. Grechkina VV, Lebedev SV, Miroshnikov IS, Ryazanov VA, Sheida EV, Korolev VL. Justification of rational and safe biotechnological methods of using fat additives from vegetable raw materials. IOP Conf. Series: Earth and Environmental Science: International Conference on World Technological Trends in Agribusiness; 2020 4-5 July, Omsk City, Western Siberia, Russian Federation. Bristol, England: IOP Publishing; 2021;624:012160. doi: 10.1088/1755-1315/624/1/012160
  14. Hales KE, Foote AP, Brown-Brandl TM, Freetly HC. The effects of feeding increasing concentrations of corn oil on energy metabolism and nutrient balance in finishing beef steers. J Anim Sci. 2017;95(2):939-948. doi: 10.2527/jas.2016.0902
  15. Huebner KL, Martin JN, Weissend CJ, Holzer KL, Parker JK, Lakin SM, Doster E, Weinroth MD, Abdo Z, Woerner DR, Metcalf JL, Geornaras I, Bryant TC, Morley PS, Belk KE. Effects of a Saccharomyces cerevisiae fermentation product on liver abscesses, fecal microbiome, and resistome in feedlot cattle raised without antibiotics. Sci Rep. 2019;9:2559. doi: 10.1038/s41598-019-39181-7
  16. Makaeva A, Duskaev G, Nurzhanov B, Rysaev A, Rakhmatullin S, Inchagova K, Rayzanov V. PSXII-27 The effect of Quercus cortex extract added separately or with probiotic to ruminal fluid of cattle (in vitro) on microbiota and fermentation characteristics. Journal of Animal Science. 2019;97(3):413. doi: https://doi.org/10.1093/jas/skz258.819
  17. Morgavi DP, Forano E, Martin C, Newbold CJ. Microbial ecosystem and methanogenesis in ruminants. Animal. 2010;4(7):1024-1036. doi:10.1017/S1751731110000546
  18. Ramos SC, Jeong CD, Mamuad LL, Kim SH,  Kang SH,  Kim ET,  Cho Y I,  Lee SS,   Lee SS. Diet transition from high-forage to high-concentrate alters rumen bacterial community composition, epithelial transcriptomes and ruminal fermentation parameters in dairy cows. Animals (Basel). 2021;11(3):838. doi: 10.3390/ani11030838
  19. Salles MSV,  Abreu LFD,   Júnior LCR,  César MC,  Guimarães JGL,  Segura JG,  Rodrigues C,  Zanetti MA,   Pfrimer K,  Netto A.  Inclusion of sunflower oil in the bovine diet improves milk nutritional profile. Nutrients. 2019;11(2):481. doi: 10.3390/nu11020481
  20. Schneider L, Volkmann N, Kemper N, Spindler B. Feeding behavior of fattening bulls fed six times per day using an automatic feeding system. Front Vet Sci. 2020;7:43. doi: 10.3389/fvets.2020.00043
  21. Sheida EV, Lebedev SV, Gubaidullina IZ, Grechkina VV, Ryazanov VA. Biotechnological aspects of the use of vegetable oils in the production of meat products. IOP Conf. Series: Earth and Environmental Science. 2021;624:012114. doi: 10.1088/1755-1315/624/1/012114
  22. SILVA. High Quality Ribosomal RNA databases [Internet] de.NBI. German network for bioinformatics infrastructure [cited 2021 March 15] Available from: https://www.arb-silva.de
  23. Tapio I, Snelling TJ,  Strozzi F,  Wallace RJ.  The  ruminal  microbiome  associated  with methane emissions from ruminant livestock. Journal of Animal Science and Biotechnology. 2017a;8:7. doi: 10.1186/s40104-017-0141-0
  24. Tapio I, Fischer D,  Blasco L, Tapio M,  Wallace RJ,  Bayat AR, Ventto L,  Kahala M,  Negussie E, Shingfield KJ,  Vilkki J. Taxon abundance, diversity, co-occurrence and network analysis of the ruminal microbiota in response to dietary changes in dairy cows. PLoS One. 2017b;12(7):e0180260. doi: 10.1371/journal.pone.0180260
  25. U.S. National Library of Medicine. National Center for Biotechnology Information [Internet] BLAST. [cited 2021 March 15] Available from: http://blast.ncbi.nlm.nih.gov/Blast.cgi
  26. U.S. National Library of Medicine. National Center for Biotechnology Information [Internet] BLAST. Nucleotide BLAST [cited 2021 March 15] Available from: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
  27. VAMPS Visualization and Analysis of Microbial Population Structures [Internet] The Josephine Bay Paul Center. [cited 2021 March 15] Available from: http://vamps.mbl.edu
  28. VargasJE, Andrés S,  López-Ferreras L,  Snelling TJ,  Yáñez-Ruíz DR,  García-Estrada C,  López S. Dietary supplemental plant oils reduce methanogenesis from anaerobic microbial fermentation in the rumen. Sci Rep.  2020;10(1):1613.  doi: 10.1038/s41598-020-58401-z
  29. Weld KA, Armentano LE. The effects of adding fat to diets of lactating dairy cows on total-tract neutral detergent fiber digestibility: A meta-analysis. Journal of Dairy Science. 2017;100(3):1766-1779. doi: https://doi.org/10.3168/jds.2016-11500
  30. Zhang J, Kobert K, Flouri T, Stamatakis A. PEAR: A fast and accurate Illumina Paired-End reAd merger. Bioinformatics. 2014,30(5):614-620. doi: 10.1093/bioinformatics/btt593

Ryazanov Vitaly Aleksandrovich, Cand. Sci. (Agr.), Researcher of Farm Animal Feeding and Feed Technology Department named after Leushin SG., Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79, e-mail: vita7456@yandex.ru

Levakhin Georgy Ivanovich, Dr. Sci. (Biol.), Professor, Chief Researcher of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)30-81-79

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Leading Researcher, Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail: gduskaev@mail.ru

Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

Nurzhanov Baer Serekpaevich, Cand. Sci (Agr.), Senior Researcher of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., e-mail: baer.nurzhanov@mail.ru

Miroshnikov Ivan Sergeevich, Cand. Sci. (Agr.), Researcher of Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, ul. 9 Yanvarya St., tel.: 8(3532)30-81-79

Received: 24 August 2021; Accepted: 13 September 2021; Published: 30 September 2021

Download