Yulia V Kilyakova, Elena P Miroshnikova, Azamat E Arinzhanov, Maria S Arinzhanova

Animal Husbandry and Fodder Production. 2022. Vol. 105, no 3. Р. 115-125.

doi:10.33284/2658-3135-105-3-115

Original article

Influence of phytobiotic feed additives on growth and morphobiochemical parameters of fish blood

 Yulia V Kilyakova1, Elena P Miroshnikova2, Azamat E Arinzhanov3, Maria S Arinzhanova4

1,2,3Orenburg State University, Orenburg, Russia

4Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1fish-ka06@mail.ru, https://orcid.org/0000-0002-2385-264X

2elenaakva@rambler.ru, https://orcid.org/0000-0003-3804-5151

3arin.azamat@mail.ru, https://orcid.org/0000-0001-6534-7118

4marymiroshnikova@mail.ru, https//orcid.org/0000-0003-1898-9307

Abstract. The paper presents the results of a study on the effect of various phytobiotic feed additives, Probiocide®-Phyto (LLC BIOTROF, Russia), Intebio (LLC BIOTROF, Russia), Butitan (LLC[1] SIVETRA-AGRO), Russia) on the productivity of carp and on the morphological and biochemical parameters of blood. The growth-stimulating effect was recorded in all experimental groups. In the course of the research, it was found that the inclusion of phytobiotic feed additives in the diet of fish is accompanied by an increase in growth intensity up to 8.8% (Intebio), up to 9.9% (Probiocide®-Phyto) and up to 11.7% (Butitan), relative to the control group. An analysis of the morphological composition of the blood showed no negative impact on the health of the experimental fish against the background of various phytobiotic feed additives used. An analysis of the biochemical parameters of the blood serum of fish showed more excellent values ​​of the experimental groups compared to the control. A significant increase in glucose levels was recorded in the groups receiving the phytobiotic supplement «Probiocid-Phyto» and «Intebio» by 45.2% (P≤0.01) and 9.9% (P≤0.01), respectively. Total bilirubin in all experimental groups was significantly higher than the control: in group I - 4 times (Р≤0.001), in group II - by 45.5% (Р≤0.05), in group III - 45.5% (P≤0.05). The analysis of transaminases established a significant decrease in the activity of alanine aminotransferase and aspartate aminotransferase in the group with the addition of "Intebio" by 22.6% (P≤0.05) and 22.5% (P≤0.01), respectively, relative to control values. A decrease in AST was recorded in the group with the addition of "Butitan" by 14.4% (P≤0.05). A significant increase in iron content in the blood serum of carp (Р≤0.001) was established: in group I - 2.5 times, in group II - 2 times and in group III - 3.7 times, relative to the control. In addition, in the I experimental group, an increase in magnesium and phosphorus was recorded relative to the control values ​​by 56.9% (P≤0.05) and 68.9% (P≤0.01), and in the II group by 2 times (P≤0.01) and 2.4 times (P≤0.001), respectively.

Keywords: carp, feeding, phytobiotic preparations, phytobiotics, blood morphological parameters, blood biochemical parameters

Acknowledgments: the work was supported by the Russian Science Foundation, Project No. 22-26-00281.

For citation: Kilyakova YuV, Miroshnikova EP, Arinzhanov AE, Arinzhanova MS. Influence of phytobiotic feed additives on growth and morphobiochemical parameters of fish blood. Animal Husbandry and Fodder Production. 2022; 105(3):115-125. (In Russ.). https://doi.org/10.33284/2658-3135-105-3-115

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 Information about the authors:

Yulia V Kilyakova, Cand. Sci. (Biology), Associate Professor, Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-961-920-40-64.

Elena P Miroshnikova, Dr. Sci. (Biology), Professor, Head of the Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-987-862-98-86.

Azamat E Arinzhanov, Cand. Sci. (Agriculture), Associate Professor, Department of Biotechnology of Animal Raw Materials and Aquaculture, Orenburg State University, 13 Pobedy Ave, Orenburg, 460018, tel.: 8-922-806-33-43.

Maria S Arinzhanova, 2st year postgraduate student, Junior Researcher, Department of Farm Animal Feeding and Feed Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 8-922-867-57-10.

The article was submitted 31.08.2022; approved after reviewing 09.09.2022; accepted for publication 12.09.2022.

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