Tatyana Yu Saprikina, Alexander Yu Krivoruchko, Olesya A Yatsyk, Olga N Krivoruchko

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 2. Р. 30-42.

 

doi:10.33284/2658-3135-106-2-30

 

Original article

Search for new candidate genes affecting fat thickness in Jalgin Merino sheep using a genome-wide association study

 

Tatyana Yu Saprikina1,5, Alexander Yu Krivoruchko2,6, Olesya A Yatsyk3,7, Olga N Krivoruchko4

1,2,3,4North Caucasus Federal Agrarian Research Centre, Mikhailovsk, Russia

5,6,7North Caucasus Federal University, Stavropol, Russia

1,5saprikina.tanya@mail.ru, https://orcid.org/0000-0002-5306-795X

2,6rcvm@yandex.ru, https://orcid.org/0000-0003-4536-1814

3,7malteze@mail.ru, https://orcid.org/0000-0003-2730-2482

4rcvm@yandex.ru, https://orcid.org/0000-0003-4536-1814

 

Abstract. The growing demand for mutton requires modern breeding solutions aimed at increasing the meat productivity of sheep of local breeds. Thanks to modern developments in molecular genetics, methods are being successfully introduced into sheep breeding, with the help of which animals are selected according to the genotype with the necessary characteristics. One of them is the technology of genome-wide association search based on the identification of the relationship between a single nucleotide substitution and the phenotype parameter. The article presents the results of an associative analysis aimed at finding new candidate genes associated with the thickness of fat in the lumbar region in Jalgin Merino sheep. A total of 18 single-nucleotide substitutions were found that were reliably associated with the studied  indicator.  According  to  the  results of the analysis of the localization of SNP in the sheep genome, 14 candidate genes have been proposed, which with a high degree of probability can control the deposition of adipose tissue in the lumbar region: MREG, KRT20, FZD6, ENSOARG00000012396, ENSOARG00000013197, ENSOARG00000026426, ASIC2, PYM1, TRPC1, UBL4B, SOX6, TCF7L2, SHLD1, UNC13C. Further research will help to study the structural features of the proposed genes in more detail, as well as to find out the mechanism of their influence on the phenotypic manifestation of economically useful traits in domestic sheep breeds.

Keywords: sheep breeding, Jalgin Merino, meat productivity, fat thickness, genome-wide association study, candidate genes, single-nucleotide polymorphism

Acknowledgments: the work was supported by the Russian Science Foundation, Project No. 22-26-20009.

For citation: Saprikina TYu, Krivoruchko AYu, Yatsyk OA, Krivoruchko ON. Search for new candidate genes affecting fat thickness in Jalgin Merino sheep using a genome-wide association study. Animal Husbandry and Fodder Production. 2023;106(2):30-42. (In Russ.). https://doi.org/10.33284/2658-3135-106-2-30

 

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Information about the authors:

Tatyana Yu Saprikina, full-time postgraduate student, Junior Researcher at the Laboratory of Genomic Breeding and Reproductive Cryobiology in Animal Husbandry, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, Russia, tel.: +7 (8655) 32-32-98; research at the Research Laboratory of Molecular genetic expertise, North Caucasus Federal University, 23 bldg., 2, Kulakov avenue, Stavropol, Russia, 355029, tel.: 8 (8652) 95‑68‑13.

Alexander Yu Krivoruchko, Dr. Sci. (Biology), Chief Researcher of the Laboratory of Genomic Breeding and Reproductive Cryobiology in Animal Husbandry, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, Russia, tel.: +7 (8655) 32-32-98; Research Associate at the Research Laboratory of Molecular Genetic Expertise, North Caucasus Federal University, 23 bldg., 2, Kulakov avenue, Stavropol, Russia, 355029, tel.: 8 (8652) 95‑68‑13.

Olesya A Yatsyk, Cand. Sci. (Biology), Senior Researcher at the Laboratory of Genomic Breeding and Reproductive Cryobiology in Animal Husbandry, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, Russia, tel.: +7 (8655) 32-32-98; research at the Research Laboratory of Molecular Genetic Expertise, North Caucasus Federal University, 23 bldg., 2, Kulakov avenue, Stavropol, Russia, 355029, tel.: 8 (8652) 95‑68‑13.

Olga N Krivoruchko, Applicant, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, Russia, 356241, tel.: +7 (8655) 32-32-98.

 

The article was submitted 24.03.2023; approved after reviewing 05.04.2023; accepted for publication 13.06.2023.

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