Ksenia S Nechitailo, Elena A Sizova, Valentina S Polyakova, Aleksandra S Mustafina, Irina V Markova

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 4. P. 121-134.

doi:10.33284/2658-3135-106-4-121

Original article

The influence of a phytobiotic extract in combination with an enzyme additive and ultrafine zinc particles on the morphobiochemical parameters of the blood of broiler chickens

 

Ksenia S Nechitailo1,6, Elena A Sizova2,7, Valentina S Polyakova3,7, Aleksandra S Mustafina4, Irina V Markova5      

1,2,3,4,5Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

6,7,8 Orenburg State University, Orenburg, Russia

1,6valyapolyakova2001@mail.ru,https://orcid.org/0009-0002-2739-0208

2,7k.nechit@mail.ru, https://orcid.org/0000-0002-8755-414X

3,8sizova.L78@yandex.ru, https://orcid.org/0000-0002-5125-5981

4vshivkovaas@mail.ru, https://orcid.org/0000-0001-9525-2822

5irinazzz88@yandex.ru

 

Abstract. The experiment studied the effect of a multicomponent feed additive, including ultrafine zinc particles (Zn), phytobiotic extract Quercus cortex (QC) and an enzyme additive (F). As a result, it was established that the feed additive used had a positive effect on the morphological and biochemical parameters of the blood of chickens. On day 21, the content of leukocytes in the “OR+F+QC+Zn” group was higher by 10.34%, erythrocytes – 19.62%, hemoglobin concentration – 13.68% (P≤0.05), hematocrit – 17, 06% (P≤0.05). Leukocyte indices: blood cell index (BCI), pseudoeosinophil-lymphocyte ratio index (PSLI), leukocyte shift index (LSI) were increased, indicating an increase in the concentration of neutrophils (pseudoeosinophils) and eosinophils in blood. At the age of 42 days, in chickens in the group “OR+F+QC+Zn” the level of total protein was higher by 8.39%, albumin – by 10.62%, AST – by 16.64% (P≤0.05). By the end of the experiment, there was a decrease in glucose levels in chickens of the “OR+F+QC+Zn” group by 15.93% (P≤0.05) and ALT by 30.25% (P≤0.05). The triglyceride content in the “OR+F+QC+Zn” group was increased by 22.61% (P≤0.05) compared to the “OR” group. Lipase concentration was reduced by 13.88% (P≤0.05). Thus, the combination of the phytobiotic extract Quercuscortex, an enzyme additive and ultrafine zinc particles can serve as an effective alternative to feed antibiotics.

Keywords: broiler chickens, feeding, hematological parameters, phytobiotic extract, Quercus cortex, enzyme additive, zinc, ultrafine particles

Acknowledgments: the work was supported by the Russian Science Foundation, Project No. 22-26-00253.

For citation: Nechitailo KS, Sizova EA, Polyakova VS, Mustafina AS, Markova IV. The influence of a phytobiotic extract in combination with an enzyme additive and ultrafine zinc particles on the morphobiochemical parameters of the blood of broiler chickens. Animal Husbandry and Fodder Production. 2023;106(4):121-134. (In Russ.). https://doi.org/10.33284/2658-3135-106-4-121

 

References

 
  1. Vertiprahov VG, Kosthcheyeva MV. Hen blood biochemistry as the base of metabolism. Poultry & Chicken Product. 2020;2:54-56. doi: 10.30975/2073-4999-2020-22-2-54-56
  2. Moiseeva AA, Prisnyi AA, Skvortsov VN, Tarasova YuV. Dynamics of leukocyte indexes in the blood  of  chicken  cross  “Haysex brown”  after  application of different doses of enrofloxacin in conditions of experimental salmonellosis. International Bulletin of Veterinary Medicine. 2021;2:66-70. doi: 10.17238/issn2072-2419.2021.2.66
  3. Tuzikov RA, Lebedev SV, Arinzhanov AE, Arinzhanova MS. Study of the effect of probiotics on productive and hematological parameters of broiler blood. Animal Husbandry and Fodder Production. 2022;105(4):195-207. doi: 10.33284/2658-3135-105-4-195
  4. Krasnolobova EP, Cheremenina NA, Kovalev SP. Diagnostic value of leukocyte indices in animals. International bulletin of Veterinary Medicine. 2018;4:140-143.
  5. Mikhaleva MS, Vertiprakhov VG. The content of erythrocytes and leukocytes in the blood of meat chickens. Poultry & Chicken Product. 2018;6:54-56. doi: 10.30975/2073-4999-2018-20-6-54-56
  6. Sadovnikov NV, Pridybaylo ND, Vereshchak NA, Zaslonov AS. General and special methods for studying the blood of industrial cross birds. Ekaterinburg-SPb.: UrGSHA-AVIVAC; 2009;85 p.
  7. Lebedev SV, Shoshina OV, Grechkina VV et al. Evaluation of the effect of chromium picolinate from the diet on biomorphological parameters and elemental composition of blood of bulls. Animal Husbandry and Fodder Production. 2022;105(4):181-194. doi: 10.33284/2658-3135-105-4-181
  8. Petrusha YuK, Lebedev SV, Grechkina VV. Phytobiotics in poultry feeding (review). Animal Husbandry and Fodder Production. 2022.105(1):103-118. doi: 10.33284/2658-3135-105-1-103
  9. Ryabcev PS. Effect of mitophen on hematological leukocyte indices of broiler chickens. International Journal of Veterinary Medicine. 2021;4:60-63. doi: https://doi.org/10.52419/issn2072-2419.2021.4.60
  10. Sheida EV, Lebedev  SV.  Influence  of  Cr2O3  UFP  on  fermentation  processes  in  rumen of  ruminants  in  in  vitro  experiments. Animal Husbandry and Fodder Production. 2023;106(3):8-20. doi: https://doi.org/10.33284/2658-3135-106-3-8
  11. Abang FBP, Anoh KU, Izuki ED, Nsa EE and Ijoko N. Productive performance and hematological indices of broiler chicks fed biodegraded cassava root.Online J Anim Feed Res. 2023;13(4):274-278. doi: https://dx.doi.org/10.51227/ojafr.2023.41
  12. Abd El-Ghany WA, Shaalan M, Salem MH. Nanoparticles applications in poultry production: an updated review. World's Poultry Science Journal. 2021;77(4):1001-1025. doi: 10.1080/00439339.2021.1960235
  13. Abd El-Hack ME, Alaidaroos BA, Farsi RM, Abou-Kassem DE, El-Saadony MT, Saad AM, Shafi ME, Albaqami NM, Taha AE, Ashour EA. Impacts of supplementing broiler diets with biological curcumin, zinc nanoparticles and Bacillus licheniformis on growth, carcass traits, blood indices, meat quality and cecal microbial load. Animals. 2021;11(7):1878. doi: https://doi.org/10.3390/ani11071878
  14. Abd El-Hack ME, El-Saadony MT, Salem HM, El-Tahan AM, Soliman MM, Youssef GBA, Taha AE, Soliman SM, Ahmed AE, El-Kott AF, Al Syaad KM, Swelum AA. Alternatives to antibiotics for organic poultry production: types, modes of action and impacts on bird's health and production. Poultry Science. 2022;101(4):101696. doi:https://doi.org/10.1016/j.psj.2022.101696
  15. Bagno O, Shevchenko S, Shevchenko A et al. Physiological status of broiler chickens with diets supplemented with milk thistle extract. Veterinary World. 2021;14(5):1319-1323. doi: 10.14202/vetworld.2021.1319-1323
  16. Basit MA, Kadir AA, Loh TC, Aziz SA, Salleh A, Kaka U, Idris SB. Effect of inclusion of different doses of Persicariaodorata leaf meal (POLM) in broiler chicken feed on biochemical and haematological blood indicators and liver histomorphological changes. Animals. 2020;10(7):1209. doi: https://doi.org/10.3390/ani10071209
  17. Buryakov NP, ZagarinAYu, Fathala MM, Aleshin DE. The role of supplementing a complex phytobiotic feed additive containing (Castanea sativa mill)extract in combination with calcium butyrate, zinc-methionine and essential oils on growth indicators, blood profile and carcass quality of broiler chickens. Veterinary Sciences. 2023;10(3):212. doi: https://doi.org/10.3390/vetsci10030212
  18. Chodkowska KA, Abramowicz-Pindor PA, Tuśnio A, Gawin K, Taciak M, Barszcz M. Effect of phytobioticcomposition on production parameters, oxidative stress markers and myokine levels in blood and pectoral muscle of broiler chickens. Animals. 2022;12(19):2625. doi: https://doi.org/10.3390/ani12192625
  19. Dean L. Blood Groups and Red Cell Antigens.In:1. Blood and the cells it contains. National Center for Biotechnology Information (US), Bethesda (MD); 2005: 1-10.
  20. Dinani OP, Tyagi PK, Tyagi JS, Bhanja SK, Rokade JJ. Effect of feeding rice gluten meal with and without enzymes on hematobiochemical profile of broiler chickens. Veterinary World. 2020;13(10):2062-2069. doi: 10.14202/vetworld.2020.2062-2069
  21. Granstad S, Kristoffersen AB, Benestad SL, Sjurseth SK, David B, Sørensen L, Fjermedal A, Edvardsen DH, Sanson G, Løvland A, Kaldhusdal M. Effect of feed additives as alternatives to in-feed antimicrobials on production performance and intestinal Clostridium perfringenscounts in broiler chickens. Animals. 2020;10(2):240. doi: https://doi.org/10.3390/ani10020240
  22. Hidayat C, Sumiati, Jayanegara A, Wina E. Effect of zinc addition on the immune response and production performance of broilers: a meta-analysis. Asian-Australas J Anim Sci. 2020;33(3):465-479. doi: 10.5713/ajas.19.0146
  23. Jaber SA. In vitro alpha-amylase and alpha-glucosidase inhibitory activity and in vivo antidiabetic activity of Quercuscoccifera (Oak tree) leaves extracts. Saudi Journal of Biological Sciences. 2023;30(7):103688. doi: https://doi.org/10.1016/j.sjbs.2023.103688
  24. Javaid A, Younas F, Ullah I, Yasinzai M. Impact of an indigenously produced multi-enzyme complex from Bacillus subtilis KT004404 on growth and blood parameters in broiler chicken. PLoS One. 2022;17(7):e0271445. doi: 10.1371/journal.pone.0271445
  25. Krauze M, Cendrowska-Pinkosz M, Matuseviĉius P, Stępniowska A, Jurczak P, Ognik K. The effect of administration of a phytobioticcontaining cinnamon oil and citric acid on the metabolism, immunity, and growth performance of broiler chickens. Animals. 2021;11(2):399. doi: 10.3390/ani11020399
  26. Moiseeva AA, Prisnyi AA, Skvortsov VN. Dynamics of WBC indices in poultry following enrofloxacin administration: comparative analysis. Siberian Journal of Life Sciences and Agriculture. 2022;14(5):63-76. doi: https://doi.org/10.12731/2658-6649-2022-14-5-63-76
  27. Rahimi G, Mohammad KS, Zarei M, Shokoohi M, Oskoueian E, Moghaddam-Poorbagher MR, Karimi E. Zinc oxide nanoparticles synthesized using Hyssopus Officinalis L.extract induced oxidative stress and changes the expression of key genes involved in inflammatory and antioxidant Systems. Biological Research. 2022;55:24. doi: https://doi.org/10.1186/s40659-022-00392-4
  28. Ryazanov V, Duskaev G, Sheida E, Nurzhanov B, Kurilkina M. Rumen fermentation, methane concentration, and blood metabolites of cattle receiving dieteticalphytobiotic and cobalt (II) chloride. Veterinary World. 2022;15(11):2551-2557. doi: 10.14202/vetworld.2022.2551-2557
  29. Sizova Е, Miroshnikov S, Lebedev S, Usha B, Shabunin S. Use of nanoscale metals in poultry diet as a mineral feed additive. Animal Nutr. 2020;6(2):185-191. doi: 10.1016/j.aninu.2019.11.007
  30. 30. Sookoian S, Pirola CJ. Liver enzymes, metabolomics and genome-wide association studies: from systems biology to the personalized medicine. World J Gastroenterol. 2015;21(3):711-725. doi: 3748/wjg.v21.i3.711
  31. Ștefănescu R, Ciurea CN, Mare AD, Man A, Nisca A, Nicolescu A, Mocan A, Babotă M, Coman NA, Tanase C. Quercusrobur older bark—a source of polyphenolic extracts with biological activities. Applied Sciences. 2022;12(22):11738. doi: https://doi.org/10.3390/app122211738
 

Information about the authors:

Ksenia S Nechitailo, Cand. Sci. (Biology), Researcher of the Centre «Nanotechnologies in Agriculture», Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 January St., Orenburg, 460000; Teacher at the Scientific and Educational Center «Biological systems and nanotechnologies», Orenburg State University, 13, Pobedy Ave., Orenburg, 460018, cell.: 8-905-893-55-99.

Elena A Sizova, Dr. Sci. (Biology), Head of the Centre «Nanotechnologies in Agriculture», Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 January St., Orenburg, 460000; Professor of the Scientific and Educational center "Biological systems and nanotechnologies, Orenburg State University, 13, Pobedy Ave., Orenburg, 460018, cell.: 8-912-344-99-07.

Valentina S Polyakova, Research Assistantat of the Centre «Nanotechnologies in Agriculture», Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 January St., Orenburg, 460000; master's student, Faculty of Chemistry and Biology, Orenburg State University, 13, Pobedy Ave., Orenburg, 460018, cell.: 8-987-891-73-65.

Aleksandra S Mustafina, Cand. Sci (Agriculture), Junior Researcher of the Testing Center of the Central Collective Use Center, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, cell.: 8-912-340-21-10.

Irina V Markova, Cand. Sci (Biology), Head of Scientific and Educational Center, Researcher, Department of Technology for Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000, сell.: 8-961-047-40-26.

The article was submitted 20.11.2023; approved after reviewing 04.12.2023; accepted for publication 11.12.2023.

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