Anastasia M Gvozdikova, Oksana B Polivanova, Daria G Fedorova

Animal Husbandry and Fodder Production. 2024. Vol. 107, no. 1. Р. 22-30.

 

doi:10.33284/2658-3135-107-1-22

 

Original article

Features of the effect of biosynthesized nanopowders on callus cultures in vitro

 

Anastasia M Gvozdikova1,4, Oksana B Polivanova2, Daria G Fedorova3

1Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

2Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev, Moscow, Russia

3,4Orenburg State University, Orenburg, Russia

1,4anastasiaporv@mail.ru, https://orcid.org/orcid.org/0000-0002-7981-7245

2polivanovaoks@gmail.com, https://orcid.org/orcid.org/0000-0002-3992-5452

3daryaorlova24@rambler.ru, https://orcid.org/0000-0002-5323-4965

 

Abstract. The study is devoted to biosynthesized nanoparticles (NPs) of zinc ferrite (ZnFe2O4) in terms of their effect on callus induction and shoot regeneration of basil crops (Ocimum basilicum L.) in vitro. In vitro derived plant cultures are attracting increasing interest worldwide due to their potential as elicitors of secondary metabolites with antioxidant properties. In the present study, different concentrations of ZnFe2O4 NPs and plant growth regulators (cytokines and auxins) were added to cultures in vitro for sustainable biomass production of basil callus cultures O. basilicum. The greatest accumulation of fresh biomass (1.50±0.06 g) and growth index (3.55) of callus were observed in cultures grown in vitro on MS medium (Murashige-Skoog) with auxin - 2 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid) and ZnFe2O4 NPs of 25 μg/l. At the same time, zinc ferrite at a concentration of 25 μg/l significantly affected the proliferation of callus tissue, which was more homogeneous compared to other media options, more anthocyanin-colored, the largest and very similar in appearance to the control (MS+2 mg/l 2,4-D).

Keywords: callus, basil, antioxidants, auxins, cytokinin, nanopowders

Acknowledgments: the   work  was  supported  by  the  Russian  Science  Foundation,  Project  No. 23-76-10060.

For citation: Gvozdikova AM, Polivanova OB, Fedorova DG. Features of the effect of biosynthesized nanopowders on callus cultures in vitro. Animal Husbandry and Fodder Production. 2024;107(1):22-30. (In Russ.). https://doi.org/10.33284/2658-3135-107-1-22

 

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Information about the authors:

Anastasia M Gvozdikova, Cand. Sci. (Biology), Senior Researcher of Laboratory of Biological Testing and Expertise, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000; Senior Researcher at the Research Center Biological Systems and Nanotechnologies, Orenburg State University, 13 Pobedy Ave., Orenburg, 460018, tel.: 89822084220.

Oksana B Polivanova, Cand. Sci. (Biology), Associate Professor of the Department of Biotechnology, Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev, 127434, Timiryazevskaya st., 49, Moscow, 127434, tel.: 89689874703.

Daria G Fedorova, Cand. Sci. (Biology), Senior Researcher, Associate Professor of the Department of Biology and Soil Science, Head of the Scientific Group of the Botanical Garden,  Orenburg State University, 13 Pobedy Ave., Orenburg, 460018, tel.: 89878918303.

The article was submitted 10.11.2023; approved after reviewing 22.01.2024; accepted for publication 18.03.2024.

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