Elena N Konovalova, Olga S Romanenkova, Elena A Gladyr

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 1. P. 42-50.

                                                                                   

doi:10.33284/2658-3135-107-1-42

 

Original article

The analysis of the Russian beef cattle population on polymorphism of CAPN1 gene

 

Elena N Konovalova1, Olga S Romanenkova2, Elena A Gladyr3 

1,2,3 Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, Dubrovitsy, Russia

1konoval-elena@yandex.ru, https://orcid.org/0000-0002-2170-5259

2ksilosa@gmail.com, https://orcid.org/0000-0002-2682-6164 3elenagladyr@mail.ru, https://orcid.org/0000-0002-5210-8932  

Abstract. The conducted studies were aimed at identification of genetic markers that affect the tenderness of beef as the most important characteristic that determines taste. In the course of works, the test systems for the identification of the allele variants of polymorphism of CAPN1 genes – CAPN1_530 and CAPN1_4751, based on PCR-RFLP and RT-PCR methods were created. Genotyping of Aberdeen-Angus cattle populations (n pop No. 1=140, n pop No. 2=20) and Galloway (n=100) breeds according to the studied polymorphisms showed the presence of pop No. 1 in Aberdeen cattle genotypes of the preferred allele C-CAPN1_4751 at a frequency of 0.44. It is noteworthy that in this population, there were no animals homozygous for the C-CAPN1_4751 allele with a sufficiently high proportion of heterozygotes (122 animals out of 140, which was 87.1%).  No animals carrying the C-CAPN1_4751 allele were found in the Galloway breed population. The G-CAPN1_530 allele, which is desirable for meat tenderness, was not identified in any of the studied populations. Due to the large influence of the CAPN1 gene on the tenderness of meat, as well as the revealing of the genetic variability for CAPN1_4751 among the Russian populations of beef cattle, we consider it advisable to conduct further research on a larger number of animals. This will contribute to the search for additional genetic markers of meat productivity with the prospect of their introduction into genomic selection systems to increase the accuracy of genomic forecasting in beef cattle breeding.

Keywords: cattle, beef breeds, productivity markers, meat tenderness, gene polymorphisms, CAPN1, CAPN1_530, CAPN1_4751

Acknowledgments: the work was supported by the Russian Science Foundation, Project No 23-26-00176, https://rscf.ru/project/23-26-00176

For citation: Konovalova EN, Romanenkova OS, Gladyr EA. The analysis of the Russian beef cattle population on polymorphism of CAPN1 gene. Animal Husbandry and Fodder Production. 2024;107(1):42-50. (In Russ.). https://doi.org/10.33284/2658-3135-107-1-42

 

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Information about the authors:

Elena N Konovalova, Cand. Sci. (Biology), senior researcher, Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, cell: +79057256390.

Olga S Romanenkova, Cand. Sci. (Biology), researcher, Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, cell: +7(4967)651102.

Elena A Gladyr, Cand. Sci. (Biology), leading researcher, chef of the Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, cell: +7(4967)651102.

The article was submitted 17.11.2023; approved after reviewing 15.01.2024; accepted for publication 18.03.2024.

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