Alexander Yu Krivoruchko, Antonina V Skokova, Larisa N Skorykh, Anastasia A Kanibolotskaya, Olga N Krivoruchko

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 2. Р. 71-84.

doi:10.33284/2658-3135-107-2-71

Original article

The use of microsatellite loci for genetic identification of wool sheep in the Stavropol Territory

 

Alexander Yu Krivoruchko1,6, Antonina V Skokova2, Larisa N Skorykh3,

Anastasia A Kanibolotskaya4, Olga N Krivoruchko5

1,2,3,4,5North Caucasus Federal Agrarian Research Centre, Mikhaylovsk, Russia

6North Caucasus Federal University, Stavropol, Russia

1,6rcvm@yandex.ru, https://orcid.org/0000-0003-0130-3639

2antoninaskokova@mail.ru, https://orcid.org/0000-0002-2193-7498

3smu.sniizhk@yandex.ru, https://orcid.org/0000-0002-6090-4453

4dorohin.2012@inbox.ru, https://orcid.org/0000-0003-3003-4175

5lgsvniiok@yandex.ru, https://orcid.org/0009-0005-2051-007х

 

Abstract. Microsatellite markers are widely used as a useful tool for measuring genetic diversity and divergence within and between populations of different animal species, including sheep. The purpose of this study was to study the intra- and interbreed genetic diversity of four breeds of sheep bred in the territory of the Stavropol territory using microsatellite markers. The material for the study was blood taken from 179 heads of sheep of 4 breeds: Manych merino (n=58); Soviet merino (n=20); North Caucasian meat-wool (n=41); Caucasian (n=60). Sheep were genotyped using 12 microsatellite loci recommended by the International Society of Animal Genetics (ISAG). The analysis of the average number of alleles per locus showed a high level of genetic diversity of the studied sheep breeds, on average 7...12 alleles. The number of effective alleles in loci varied from 4 for the Soviet merino sheep to 6 for the Caucasian breed sheep. The highest frequency of occurrence was characterized by alleles 189 of the MAF214 locus (68%) in the Manych merino and 154 of the INRA172 locus (65%) in the Soviet merino. Alleles 209 were identified in the CSRD247 locus, 166 in the INRA172 locus and 129 in the MAF65 locus, found only in the Manych Merino sheep, with a frequency of occurrence of 26, 28 and 40%, respectively. Allele 97 in the OarFCB20 locus with an occurrence frequency of 20% was found only in the Soviet Merino sheep. The observed (Ho) and expected heterozygosity (He) indicators for 12 microsatellite loci were 0.8 and 0.78. PIC values ​​ranged from 0.42 (MAF214) to 0.87 (INRA5) with an average value of 0.7 for all loci, indicating the high information value of microsatellites as molecular genetic markers. The results of this study can be used in drawing up breeding programs and plans for the conservation of genetic resources of local sheep breeds to maintain an adequate level of biodiversity in sheep farming.

Keywords: sheep, genotype, microsatellite analysis, locus, allele, genetic diversity

For citation: Krivoruchko AYu, Skokova AV, Skorykh LN, Kanibolotskaya AA, Krivoruchko ON. The use of microsatellite loci for genetic identification of wool sheep in the Stavropol Territory. Animal Husbandry and Fodder Production. 2024;107(2):71-84. (In Russ.). https://doi.org/10.33284/2658-3135-107-2-71

 

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Information about the authors:

Alexander Yu Krivoruchko, Dr. Sci. (Biology), Chief Researcher of the Department of Genetics and Biotechnology, North Caucasian Federal Scientific Agrarian Center, 49 Nikonov str., Mikhailovsk, Stavropol Territory, Russian Federation, 356241; Professor of the North Caucasus Caucasian Federal University, Kulakova str. 2, Stavropol, 355000, tel.: +79188814327.

Antonina V Skokova, Cand. Sci. (Biology), Senior Researcher at the Department of Genetics and Biotechnology, North Caucasus Federal Scientific Agrarian Center, 49 Nikonov str., Mikhailovsk, Stavropol Territory, 356241, tel.: +79627404231.

Larisa N Skorykh, Dr. Sci. (Biology), Associate Professor, Chief Researcher of the Department of Genetics and Biotechnology, North Caucasus Federal Scientific Agrarian Center, 49 Nikonov str., Mikhailovsk, Stavropol Territory, 356241, tel.: 8(8652)71-81-55.

Anastasia A Kanibolotskaya, Cand. Sci. (Biology), Senior Researcher at the Department of Genetics and Biotechnology, North Caucasus Federal Scientific Agrarian Center, 49 Nikonov str., Mikhailovsk, Stavropol Territory, 356241, tel.: +7 9614569925.

Olga N Krivoruchko, Postgraduate student of the Department of Genetics and Biotechnology, North Caucasus Federal Scientific Agrarian Center, 49 Nikonov str., Mikhailovsk, Stavropol Territory, 356241.

The article was submitted 05.04.2024; approved after reviewing 26.04.2024; accepted for publication 10.06.2024.

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