Yuri A Sechnev, Elena V Sheida, Olga V Kvan, Yaroslav A Sizentsov

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 2. P. 170-178.

doi:10.33284/2658-3135-107-2-170

Original article

The nature of barley nutrients fermentation using exoenzymes

 

Yuri A Sechnev1, Elena V Sheida2, Olga V Kvan3, Yaroslav A Sizentsov4

1,2,3,4Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1y.sechnev@outlook.com, https://orcid.org/0009-0009-5920-1185

2,5elena-shejjda@mail.ru, https://orcid.org/0000-0002-2586-613X

3kwan111@yandex.ru, https://orcid.org/0000-0003-0561-7002

4yasizen@mail.ru, https://orcid.org/0000-0002-1871-0225

 

Abstract. Fibrolytic enzyme complexes are widely used in the feeding of farm animals in order to increase the digestibility and effectiveness of nutrients through their use in the preparation of feed. The article presents the results of research on the enrichment of grain feeds by fermentation of substrates using various doses of fibrolytic enzymes. The purpose of the research is to study the effects of fibrolytic enzyme preparations on changes in the chemical composition of grain forage. Fungal alpha-Amylase (Amilorizin), an enzyme derived from Aspergillus oryzae strain, was used as an enzyme preparation. The study used different varieties of barley cultivated in our center with different nutrient contents. The enzyme preparation  was  used  in doses of 25 and 50 mg / kg of vegetable raw materials. Barley grain was hung 200 g each in sealed plastic containers, the required volume of enzyme preparation was introduced and placed in a thermostat for incubation at a temperature of 39 °C for 24 hours. The degradation of barley nutrients was recorded after 3, 6, 9, 12 and 24 hours. The studies of the digestibility of dry matter (DM) of barley were carried out by the in vitro method using the incubator "ANKOM Daisy II" (modifications D200 and D200I). The use of a fibrolytic enzyme in the fermentation of a grain substrate contributes to the decomposition of raw fiber of barley up to 6%, an increase in fat content and an improvement in the digestibility of dry matter by 5.4-6.8% in the "artificial rumen" system.

Keywords: barley grain, fermentation, amylorizine, dry matter, crude fat, crude protein, crude fiber

Acknowledgments: the  work  was  supported by the Russian Science Foundation, Project  No. 23-16-00061.

For citation: Sechnev YuA, Sheida EV, Kvan OV, Sizentsov YaA. The nature of barley nutrients fermentation using exoenzymes. Animal Husbandry and Fodder Production. 2024;107(2):170-178. (In Russ.). https://doi.org/10.33284/2658-3135-107-2-170

 

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Information about the authors:

Yuri A Sechnev, postgraduate student of the Department of feeding of agricultural animals and feed Technology named after S.G. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 89112151912.

Elena V Sheida, Dr. Sci. (Biology), Researcher at the Laboratory of Biological Tests and Examinations, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 8-922-862-64-02.

Olga V Kvan, Cand. Sci. (Biology), Acting Head of the Department of Animal Feeding and Feed Technology named after S.G. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, phone: 89225485657.

Yaroslav A Sizentsov, post-graduate student, Junior Research Associate of the Department of Animal Feeding and Feed Technology named after S.G. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 89538391917.

The article was submitted 30.05.2024; approved after reviewing 04.06.2024; accepted for publication 10.06.2024.

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