Mitrofanova OV, Solovieva LI , Kuzicheva YY.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 3. Р. 126-135.

doi:10.33284/2658-3135-108-3-126

Original article

Allelic diversity according to microsatellite (STR) loci in the Holstein cows

Olga V Mitrofanova1, Lyubov I Solovieva2, Yulia Y Kuzicheva3

1,2,3All-Russian Research Institute of Genetics and Breeding of Farm Animals, branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Animal Husbandry - VIZh named after Academician L.K. Ernst", St. Petersburg, Russia

1mo1969@mail.ru, https://orcid.org/0000-0003-4702-2736

2lyuba.vasileva1992@mail.ru, https://orcid.org/0009-0004-9542-1146

3kuzicheva@list.ru, https://orcid.org/0009-0006-2459-495X

Abstract. Due to the limited number of sires used in the Holstein herds, the genetic diversity monitoring remains relevant. Microsatellite (STR) markers designed to control origin are used for this purpose. The aim of our work was to assess the allelic diversity of microsatellite loci in the Holstein cows at  the  Prinevskoye  Breeding  Farm, Leningrad Region. Capillary electrophoresis was used to analyze 211 cows  according to 15 loci. A total of 106 alleles were identified. Highly polymorphic loci included TGLA227  and  TGLA53,  in  which  11  allelic variants were identified, as well as the TGLA122 locus with 10 allelic variants. The highest frequency (0.701) was noted for allele 248 of the SPS115 locus. High values оf observed heterozygosity (0.85) were found for the TGLA53 and TGLA122 loci, and the lowest value (0.42) was found for the SPS115 locus. Seven loci with reduced heterozygosity were found, which may indicate the presence of consanguineous matings in the population. Positive FST values were noted for the loci BM1818, BM1824, CSSM66, ETH3, ETH225, SPS115, TGLA126, which also indicates a lack of heterozygotes in them. In the studied sample, a deviation of allele frequencies from the Hardy-Weinberg equilibrium was noted for almost all loci except SPS115.

Keywords: cattle (Bos taurus), genetic markers, microsatellites (STR), allele pool, polymorphism

Acknowledgments: the work was performed in accordance to the plan of research works for 2024 of the RRIFAGB (No. FGGN-2024-0015).

For citation: Mitrofanova OV, Solovieva LI , Kuzicheva YY. Allelic diversity according to microsatellite (STR) loci in the Holstein cows. Animal Husbandry and Fodder Production. 2025;108(3):126-135. (In Russ.). https://doi.org/10.33284/2658-3135-108-3-126

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Information about the authors:

Olga V Mitrofanova, Cand. Sci. (Biology), leading biologist, All-Russian Research Institute of Genetics and Breeding of Farm Animals, branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Animal Husbandry - VIZh named after Academician L.K. Ernst", St. Petersburg, Tyarlevo, Moscow highway, 55a, 196625, +7(921)7964163.

Lubov I Solovieva, leading specialist, All-Russian Research Institute of Genetics and Breeding of Farm Animals, branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Animal Husbandry - VIZh named after Academician L.K. Ernst", St. Petersburg, Tyarlevo, Moscow highway, 55a, 196625, +7(911)0855565.

Yulia Y Kuzicheva, leading specialist, All-Russian Research Institute of Genetics and Breeding of Farm Animals, branch of the Federal State Budgetary Scientific Institution "Federal Research Center for Animal Husbandry - VIZh named after Academician L.K. Ernst", St. Petersburg, Tyarlevo, Moscow highway, 55a, 196625, +7(911)2344258.

The article was submitted 24.02.2025; approved after reviewing 24.04.2025; accepted for publication 15.09.2025.

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