Deniskova TE, Solovieva AD, Koshkina OA, Yakovleva OS, Dotsev AV, Zinovieva NA.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 4. Р. 151-161.

doi:10.33284/2658-3135-108-4-151

Original article

 

Development and validation of a test system for identifying resistant haplotypes

to Maedi-Visna virus in sheep

 

Tatyana E Deniskova1, Anastasia D Solovieva2, Olga A Koshkina3, Olga S Yakovleva4,

Arsen V Dotsev5, Natalia A Zinovieva6

1,2,3,4,5,6Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, Dubrovitsy, Russia

1horarka@yandex.ru, https://orcid.org/0000-0002-5809-1262

2anastastasiya93@mail.ru, https://orcid.org/0000-0003-2628-9554

3olechka1808@list.ru, https://orcid.org/0000-0003-4830-6626

4olya111011@gmail.com

5asnd@mail.ru, https://orcid.org/0000-0003-3418-2511

6n_zinovieva@mail.ru, https://orcid.org/0000-0003-4017-6863

 

Abstract. The study focuses on the analysis of the SNP rs414338245 in the TMEM154 gene, which is associated with sheep resistance to the Visna-Maedi virus. The C allele is linked to susceptibility. A test system for PCR analysis was developed. The study of 753 animals of the Romanov breed revealed a high average frequency of the C allele (65.34%), with a maximum in the Kamchatka Territory (100%). The resistant TT genotype was rare (11.82%), while the infection-predisposing CC genotype reached 42.5%. The results are consistent with international data on the role of TMEM154. The high prevalence of susceptible alleles in Russian flocks indicates the relevance of implementing marker-assisted selection to control lentiviral infections.

Keywords: sheep, Romanov breed, Maedi-Visna virus, TMEM154, genetic resistance, real-time PCR

Acknowledgments: the work was performed in accordance to the plan of research works for 2024-2026 LK Ernst Federal Research Center (No. FGGN-2024-0015).

For citation: Deniskova TE,  Solovieva AD, Koshkina OA, Yakovleva OS, Dotsev AV, Zinovieva NA. Development and validation of a test system for identifying resistant haplotypes to Maedi-Visna virus in sheep. Animal Husbandry and Fodder Production. 2025;108(4):151-161. (In Russ.). https://doi.org/10.33284/2658-3135-108-4-151

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Information about the authors:

Tatyana E Deniskova, Cand. Sci. (Biology), Leading Researcher, Small Ruminant Genetics and Genomics Group, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

Anastasia D Solovieva, Junior Researcher, Small Ruminant Genetics and Genomics Group, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

Olga A Koshkina, Cand. Sci. (Biology), Researcher, Small Ruminant Genetics and Genomics Group, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

Olga S Yakovleva, Postgraduate Student, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

Arsen V Dotsev, Cand. Sci. (Biology), Leading Researcher, Head of Laboratory of Functional and Evolutionary Animal Genomics, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

Natalia A Zinovieva, Dr. Sci. (Biology), Professor, Academician of the Russian Academy of Sciences, Director, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy village, Podolsk City district, Moscow region, 142132.

The article was submitted 12.08.2025; approved after reviewing 01.11.2025; accepted for publication 15.12.2025.

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