Gaponov NV.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 4. Р. 292-309.

doi:10.33284/2658-3135-108-4-292

Original article

Physiological status and biological and productive indicators of rainbow trout after adjusted diets with narrow-leaved lupine

Nikolay V Gaponov1

1All-Russian Scientific Research Institute of Lupine – branch of the Federal Williams Research Center of Forage Production & Agroecology, Michurinskiy, Bryansk region, Russia

1nv.1000@bk.ru, https://orcid.org/0000-0002-5086-7943

Abstract. This paper presents the results of a study of the effect of extruded narrow-leaf lupine (Lupinus angustifolius L.), both native and shell-free, as part of a complete compound feed on fish-breeding indices, blood biochemical parameters, and the profitability of rainbow trout production. It has been revealed that the inclusion of narrow-leaved lupine in the formula of compound feeds makes it possible to completely or partially replace expensive protein components of animal and plant origin in the feeding diets of rainbow trout. As a result, the experimental groups showed an increase in the average daily growth of trout compared to the control group: in the 2nd experimental group – by 5.54%, in the 3rd group – by 10.98%, and in the 4th group – by 20.88%. In addition, a decrease in the consumption of feed and nutrients required to obtain a unit of output was recorded. In the 2nd experimental group, feed costs decreased by 1.67%, in the 3rd group – by 1.92%, and in the 4th group – by 7.17% compared with the control group. A similar trend was observed with regard to exchange energy costs: in groups 2, 3 and 4, the decrease was 1.89%, 1.92% and 7.17%, respectively. Protein consumption decreased by 1.37% in group 2, by 1.54% in group 3, and by 4.69% in group 4. Biomarker analysis demonstrated an increase in enzyme activity in the blood of the experimental groups, while the indicators remained within physiological limits, indicating active trout body growth and a balanced diet. According to the economic analysis, an increase in additional profit was observed in the experimental groups: 1,047 rubles in Group 2, 1,945 rubles in Group 3, and 3,657 rubles in Group 4. At the same time, trout production profitability increased: in these groups, this indicator improved by 12%, 23%, and 44%, respectively.

 Keywords: rainbow trout, biomarker, aminotransferase, enzyme, transamination, narrow-leaved lupine, decortication

For citation: Gaponov NV. Physiological status and biological and productive indicators of rainbow trout after adjusted diets with narrow-leaved lupine. Animal Husbandry and Fodder Production. 2025;108(4):292-309. (In Russ.). https://doi.org/10.33284/2658-3135-108-4-292

References

  1. Ageeva PA, Potchutina NA. Promising forage lupine variety Uzkolistny 53. Siberian Herald of Agricultural Science. 2025;55(1-314):52-59. doi: 10.26898/0370-8799-2025-1-6
  2. Ageeva PA, Potchutina NA,  Konnova LV.  Agrobiological evaluation of the varieties and cultivars of narrow-leafed fodder lupine. Siberian Herald of Agricultural Science. 2024;54(1):71-81. doi: 10.26898/0370-8799-2024-1-8
  3. Ageeva PA, Pochutina NA, Misnikova NV. The study of the modern narrow-leafed lupin genebank for productivity elements and morpho-biological characters. Agrarian Bulletin of the Urals. 2023;23(2):41-52. doi: 10.32417/1997-4868-2023-231-02-41-52
  4. Mingazova MS, Miroshnikova EP, Kilyakova YuV, Arinzhanov AЕ. The effect of biologically active feed additives in the diet on the hematological parameters of fish. Animal Husbandry and Fodder Production. 2025;108(1):158-174. doi: 10.33284/2658-3135-108-1-158
  5. Gaponov NV. The use of white lupine to improve the productivity of rainbow trout. Zootechniya. 2023;2:23-27. doi: 10.25708/ZT.2023.95.24.006
  6. Gaponov NV. Hematological parameters of rainbow trout when white lupine is included in the structure of diets. Agricultural Journal. 2025a;3(18):89-99. doi: 10.48612/FARC/2687-1254/009.3.18.2025
  7. Gaponov NV. Physiological, biochemical and histological parameters of trout muscles when white lupine is included in the diet structure. Proceedings of the Kuban State Agrarian University. 2025b;118:269-276. doi: 10.21515/1999-1703-118-269-276
  8. Gataullina GG, Shitikova AV, Medvedeva NV. Formation of pods, seeds and yield of varieties of white lupin (Lupinus Albus L.) with a determinant type of growth. Izvestiya of Timiryazev Agricultural Academy. 2023;5:51-61. doi: 10.26897/0021-342X-2023-5-51-61
  9. Gataulina EA, Shishkina EA. Rural economy: the role of forestry and logging, fisheries and fish farming for small and medium entrepreneurship of municipalities. Economy of agricultural and processing enterprises. 2025;3:45-50. doi: 10.31442/0235-2494-2025-0-3-45-50
  10. GOST 13496.152016. Feeds, mixed feeds, feed raw material. Methods for determining the raw fat content. Implementation date 2018-01-01. Moscow: Standartinform; 2020:9 р.
  11. GOST 13496.4-2019. Fodder, мixed fodder and raw mixed fodder. Methods of nitrogen and crude protein determination. Implementation date 2020-08-01. Moscow: Standartinform; 2019:15 р.
  12. GOST 26226-95. Fodder, mixed fodder and mixed fodder raw material. Methods for determination of raw ash. Implementation date 1997-01-01. Minsk: Interstate Council for Standardization, Metrology and Certification; 2003:6 p.
  13. GOST 26570-95. Fodder, mixed fodder and mixed fodder raw material. Methods for determination of calcium. Implementation date 1997-01-01. Minsk: Interstate Council for Standardization, Metrology and Certification; 2003:13p.
  14. GOST 26657-97. Fodders, mixed fodders, mixed fodder raw materials. Methods for determination of phosphorus content. Implementation date 1999-01-01. Minsk: Interstate Council for Standardization, Metrology and Certification; 1999:68 p.
  15. GOST 31675-2012. Feeds. Methods for determination of crude fibre content with intermediate filtration. Implementation date 2013-07-01. Moscow: Standartinform; 2014:9 р.
  16. GOST 57059-2016. Feeds, compound feeds, feed raw materials. Express-method for determination of moisture. Implementation date 2017-07-01. Moscow: Standartinform; 2016:5 p.
  17. Kononenko SI, Yurina NA, Maxim EA, EV Chernyshov. Innovative feed additives for growing fish fry. Proceedings of Gorsky State Agrarian University. 2017;53(1):30-34.
  18. Inshin OV, Miroshnikova EP, Arinzhanov AE. Biological effect of activated carbon as a feed additive on the organism of rainbow trout. Animal Husbandry and Fodder Production. 2024;107(1):147-160. doi: 10.33284/2658-3135-107-1-147
  1. Ursu RV, Guseva YuA, Pigina SYu et al. Quantitative and qualitative amino acid analysis of alternative protein sources in compound feeds. Proceedings of Lower Volga Agro-University Complex: Science and Higher Education. 2022;4(68):362-369. doi: 10.32786/2071-9485-202204-44
  2. OST 15.372-87. Water for Fish Farms. General Requirements and Standards. Introduced Implementation date 04/01/1988. Moscow; 1987:19 р.
  3. Ponomarev SV, Grozescu YuN, Bakhareva AA. Fish Feed and Feeding in Aquaculture. Moscow: MORKNIGA; 2013:417 р.
  4. Karpenko LYu, Sidorova NA, Politkovskaya PA, Bakhta AA, Savushkin AI, Nikonov IN. Comparative analysis of the biochemical composition of rainbow trout blood serum under different feeding diets. International Journal of Veterinary Medicine. 2024;3:301-312. doi: 10.52419/issn2072-2419.2024.3.301
  5. Shcherbina MA. Methodical Guidelines for the Physiological Assessment of Fish Feed Nutrition. Moscow: VASHNIL. 1983;83 р.
  6. Shcherbina MA, Gamygin EA. Fish feeding in freshwater aquaculture. Moscow: VNIRO Publishing House; 2006:360 р.
  7. Shcherbina MA, Gamygin EA, Saltykova IA. Effect of extrusion on the nutritional value of fish feed raw materials. Fish industry. Series: Aquaculture. Fish feed and feeding. VNIERKH. 1996;2:1-11.
  8. Naveed N, Imtiaz A, Gohar BW. Hematological and serum biochemical reference intervals of rainbow trout, Oncorhynchus mykiss cultured in Himalayan aquaculture: Morphology, morphometrics and quantification of peripheral blood cells. Saudi Journal of Biological Sciences. 2022;29(4):2942-2957. doi: 10.1016/j.sjbs.2022.01.019
  9. Pizzagalli MD, Bensimon AD, SupertiFurga GA. Guide to plasma membrane solute carrier proteins. FEBS J. 2021;288:2784-2835. doi: 10.1111/febs.15531.
  10. Seibel H, Baвmann B, Rebl A. Blood will tell: What hematological analyses can reveal about fish welfare. Front. Vet. Sci. 2021;8;616955. doi: 10.3389/fvets.2021.616955
  11. Shitikova AV, Gataulina GG, Konstantinovich AV. Growth reactions at the initial stages of the ontogenesis of white lupin with the use of growth-regulating compounds. BIO Web of Conferences. 2024;139:05014. doi: 10.1051/bioconf/202413905014
  12. Smitt FA. Kritisk förteckning öfver de i Riksmuseum befintliga salmonider. Stockholm, Kongliga Svenska Vetenskaps-Akademien; 1886:290 p.

Information about the authors:

Nikolay V Gaponov, Cand. Sci. (Biology), Leading Researcher, Department of Lupine Processing and Food Use, All-Russian Lupine Research Institute – Branch of the Federal Williams Research Center of Forage Production and Agroecology, Bryansk region, Bryansk district, Michutinskiy village, st. Beryozovaya, 2 Russia, 241524.

 

The article was submitted 01.07.2025; approved after reviewing 26.09.2025; accepted for publication 15.12.2025.

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