Podlasova EYu.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 4. Р. 344-355.

doi:10.33284/2658-3135-108-4-344

Original article

Effect of metal chelates and sulfates on the morphometric parameters of barley seedlings

 

Ekaterina Yu Podlasova1

1Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1katerina.pryakhina@mail.ru, https://orcid.org/0000-0002-2985-198X

                                                                                

Abstract. Such trace elements as copper, zinc and manganese play an important role in plant physiology. Their use as a pre-sowing seed treatment can have both stimulating and toxic effects, depending on the chemical form and concentration. A change in the intensity of the bioluminescence of the Escherichia coli K-12 TG1 strain was used as an operational and sensitive method for assessing toxicity. The effect of chelated and sulfate forms of Cu, Zn, and Mn on the suppression of bacterial luminescence and on the morphometric parameters of spring barley seedlings was studied. The results of the study showed that Zn and Mn chelates showed relatively low toxicity to bacterial luminescence up to 50 mg/ml, whereas the death of 50% of bacteria was caused by Cu chelate - 0.781 mg/ml and Zn sulfates – 1.9*10-4 mg/ml and Cu – 0.024 mg/ml. Pre-sowing treatment of seeds with 0,1% Cu chelates increased germination by 2.5% (P≤0.05), the wet and dry weight of roots by 127.4 and 156,5% and seedlings by 94 and 127.9% relative to the control. The use of MnSO4 in concentrations of 10-5% and 10-6% increased germination by 4.5 and 5% and contributed to an increase in the wet and dry weight of roots by 10.4 and 44.5% (P≤0.05), 29.3 and 54.3%, respectively, relative to the control. Thus, the dependence of phytoxicity on the chemical form and concentration of trace elements was revealed. Sulfate forms generally showed higher toxicity than chelated forms, while low concentrations of chelates and MnSO4 had a stimulating effect on the morphometric parameters of spring barley. The results obtained are of practical importance for optimizing pre-sowing seed treatment, it is recommended to select the chemical form and certain concentrations of trace element preparations with subsequent validation in the field.

Keywords: spring barley, Hordeum vulgare, chelates, sulfates, bioluminescence, germination, seedlings, zinc, manganese, copper

Acknowledgments: the work was performed in accordance to the plan of research works for 2022-2026 FSBRI FRC BST RAS (No. FNWZ-2022-0015).

For citation: Podlasova EYu. Effect of metal chelates and sulfates on the morphometric parameters of barley seedlings. Animal Husbandry and Fodder Production. 2025;108(4):344-355. (In Russ.). https://doi.org/10.33284/2658-3135-108-4-344

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Information about the authors:

Ekaterina Yu Podlasova, Cand. Sci. (Agriculturе), Researcher at the Laboratory of Breeding and Genetic Research in Crop Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89877866593.

The article was submitted 03.10.2025; approved after reviewing 13.10.2025; accepted for publication 15.12.2025.

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