Tarasova EI, Yausheva EV, Sizova EA.

Animal Husbandry and Fodder Production. 2026. Vol. 109. No. 1. Р. 8-26.

doi: 10.33284/2658-3135-109-1-8

Original article

Polymorphism of the LRP4 gene and its influence on elemental status and milk productivity of Black-and-White cows in the Leningrad, Vologda, and Orenburg Regions

 

Ekaterina I Tarasova1, Elena V Yausheva2, Elena A Sizova3

1,2,3Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1ekivtarasova@mail.ru, https://orcid.org/0000-0001-6325-7389

2vasilena56@mail.ru, https://orcid.org/0000-0002-1589-2211

3sizova.l78@yandex.ru, https://orcid.org/0000-0002-5125-5981

Abstract. The aim of the study was to evaluate the influence of the LRP4 gene genotype and regional factors on the mineral status and milk productivity of Black-and-White cows. The object of the study were Black-and-White cows (n = 90, age: 4-5 years, second stage of lactation (100-200 days)), reared in the Leningrad, Vologda, and Orenburg regions. The live weight of animals was 550-590 kg. The elemental composition of biosubstrates (hair, blood serum) was determined by inductively coupled plasma mass spectrometry. Genotyping was performed by high-throughput sequencing using a MiSeq sequencer (Illumina, USA). Carriers of the heterozygous GA genotype of the LRP4 gene in all studied populations were characterized by significantly (P≤0.05) higher concentrations of macro- and microelements in serum (Na, V) and hair (Fe, Mn, Zn). Differences between the GA/GG genotypes in hair cobalt, chromium, aluminum, and lead levels were regional. The LRP4 gene genotype also had a statistically significant effect on average daily milk yield, with the direction of the effect varying by region. We concluded that the LRP4 gene exerted a pleiotropic effect on the mineral status and milk productivity of cows, with its effects significantly dependent on regional conditions. This should be taken into account when breeding and managing highly productive populations.

Keywords: dairy cattle, Black-and-White breed, LRP4, elemental composition, blood serum, hair, milk productivity

Acknowledgments: the work was supported in accordance to the plan of research works for 2025-2027 FSBRI FRC BST RAS (No. FNWZ-2025-0001).

            For citation: Tarasova EI, Yausheva EV, Sizova EA. Polymorphism of the LRP4 gene and its influence on elemental status and milk productivity of Black-and-White cows in the Leningrad, Vologda, and Orenburg Regions. Animal Husbandry and Fodder Production. 2026;109(1):8-26. (In Russ.). https://doi.org/10.33284/2658-3135-109-1-8

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Information about the authors:

Ekaterina I Tarasova, Junior Researcher Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

Elena V Yausheva, Cand. Sci. (Biology), Senior Researcher Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

Elena A Sizova, Dr. Sci. (Biology), Professor, Professor of the Russian Academy of Sciences, Chief Researcher Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

The article was submitted 13.01.2026; approved after reviewing 02.03.2026; accepted for publication 16.03.2026.

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