Shoshin DE, Ryazanseva KV, Nechitailo KS, Sizova EA, Brezhnev AM, Gulukin AМ.

Animal Husbandry and Fodder Production. 2026. Vol. 109. No. 2. Р. 59-77.

doi: 10.33284/2658-3135-109-2-59

 

Original article

Comparative analysis of the biological activity of ultrafine particles of various genesis

in the bacterial luminescence inhibition test

Daniil E Shoshin1,6, Kristina V Ryazantseva2, Ksenia S Nechitailo3,7, Elena A Sizova4,8,

Andrey M Brezhnev5, Alexey M Gulukin9

1,2,3,4,5 Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

6,7,8 Orenburg State University named after VА Bondarenko, Orenburg, Russia

9Federal Research Center–All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences, Moscow, Russia

1,6 daniilshoshin@mail.ru, https://orcid.org/0000-0003-3086-681X

2 reger94@bk.ru, https://orcid.org/0000-0001-5134-0396

3,7 k.nechit@mail.ru, https://orcid.org/0000-0002-8755-414X

4,8 sizova.l78@yandex.ru, https://orcid.org/0000-0002-5125-5981

5 brejnevandrej@yandex.ru, https://orcid.org/0009-0006-6434-1607

9admin@viev.ru, https//orcid.org/0000-0003-2160-4770

Abstract. The problems of spreading multidrug resistance among the pathogenic microbiota of farm animals and humans prompt an active search and certification of highly effective alternatives to antibiotic drugs. Among the latter, special attention is paid to ultrafine particles (UFPs) of various origins (physical, chemical or biological origin). At the same time, "green" UFPs are usually more biocompatible and demonstrate better antibiotic properties, which, however, largely depends on the raw materials and technology used for their synthesis. In particular, the purpose of this work was to analyze the biological activity of CuO UFPs obtained by spray drying (commercial sample) and by the recovery of copper (II) sulfate in fireweed extract (laboratory sample) in the Escherichia coli K12 TG1 bacterial luminescence inhibition test. As a result of the conducted research, it was found that "green" UFPs have more pronounced antibiotic properties than UFPs obtained by physical means. Thus, the minimum inhibitory concentration for the commercial sample was 12.5 mg/ml, while for the prototype it was 9.77×10-2, which is 128 times less, although in both cases a pronounced prolonged bactericidal effect was observed. At the same time, it was shown that the "green" UFPs, by their bactericidal activity, occupy an intermediate position between the initial metal salt and the commercial sample, although the fireweed extract used in their synthesis, on the contrary, stimulated the glow of the biosensor in the range up to 578.2% relative to the control. Thus, "green" UFPs have a clear potential for use in animal husbandry as an analogue to antibiotic drugs due to their pronounced bactericidal effect, however, before their direct introduction into feed, it is necessary to conduct a more detailed analysis of their other biological properties, such as: gene and cytotoxicity, nephrotoxicity and neurotoxicity against the background of varying physicochemical characteristics (size, shape, active surface area and its electrical properties).

Keywords: ultrafine particles, copper, cypress, bacterial luminescence, "green" synthesis

Acknowledgments: the   work  was  supported  by  the  Russian  Science  Foundation,  Project  No. 25-76-10032

For citation: Shoshin DE,  Ryazanseva  KV,  Nechitailo  KS,  Sizova EA,  Brezhnev AM, Gulukin AМ. Comparative analysis of the biological activity of ultrafine particles of various genesis in the bacterial luminescence inhibition test. Animal Husbandry and Fodder Production. 2026;109(2):59-77. (In Russ.). https://doi.org/10.33284/2658-3135-109-2-59

 

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            Information about the authors:

            Daniil E Shoshin, post-graduate student, Junior Researcher at the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., 460000, Orenburg, Russia; Assistant at the Scientific and Educational Center "Biological Systems and Nanotechnology", Orenburg State University named after VА Bondarenko, 13 Pobedy ave., Orenburg, 460018, tel.: 8-965-932-53-67.

            Kristina V Ryazantseva, Cand. Sci. (Biology), Researcher at the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., 460000, Orenburg, Russia, tel.: 8-986-775-95-45.

            Ksenia S Nechitailo, Cand. Sci. (Biology), Senior Researcher at the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., 460000, Orenburg, Russia; senior lecturer at the Scientific and Educational Center "Biological Systems and Nanotechnology", Orenburg State University named after VА Bondarenko, 13 Pobedy ave., Orenburg, 460018, tel.: 8-905-893-55-99.

            Elena A Sizova, Dr Sci. (Biology), Professor, Chief Researcher at the Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., 460000, Orenburg, Russia; Professor at the Scientific and Educational Center "Biological Systems and Nanotechnology", Orenburg State University named after named after VА Bondarenko, 13 Pobedy ave., Orenburg, 460018, tel.: 8-912-344-99-07.

            Andrey M Brezhnev, post-graduate student, Laboratory Researcher at the Department of Physiology, Biochemistry and Morphology of Animals, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., 460000, Orenburg, Russia, tel.: 8-912-844-34-59.

Alexey M Gulyukin, Dr Sci. (Veterinary), Corresponding Member of Russian Academy of Sciences, Leading Researcher, Laboratory of Epizootology, Federal Research Center–All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences, Moscow, 24, Ryazanskiy prospect, build. 1, Moscow, 109428.

The article was submitted 18.02.2026; approved after reviewing 27.05.2026; accepted for publication 15.06.2026.

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