Kalashnikov V.V., Zaytsev A.M., Atroshchenko M.M., Miroshnikov S.A., Zavyalov O.A., Frolov A.N., Kurilkina M.Ya.

Development of a method for assessment and correction of elemental status of horses: reference intervals for the content of chemical elements in hair of mane

DOI: 10.33284/2658-3135-102-1-40

UDC 636.1:577.17(470.6)

 Development of a method for assessment and correction of elemental status of horses:

reference intervals for the content of chemical elements in hair of mane

V.V. Kalashnikov1, A.M. Zaytsev1, M.M. Atroshchenko1, S.A. Miroshnikov2,3, O.A. Zavyalov2,

A.N. Frolov2, M.Ya. Kurilkina2

1FSBSI «All-Russian Research Institute of Horse Breeding»

2FSBSI «Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences»

3FSBEI HE «Orenburg State University»

 Summary. The present study is an attempt to establish the boundaries of reference intervals of the content of chemical elements in hair of Thoroughbred horses bred in a certain biogeochemical province. Hair samples were taken from the area of ​​mane in the area of ​​projection of the first cervical vertebra. The elemental composition of hair was determined according to 12 essential (Co, Cr, Cu, Fe, I, Mn, Se, Zn, Li, Ni, Si, V) and 8 toxic (Al, As, Cd, B, Pb, Sn, Sr, Hg) elements by atomic emission and mass spectrometry (AES-ICP and MS-ICP). Statistical analysis of results was carried out using Statistica 10.0 software package (Stat Soft Inc., USA). Statistical comparison of results was carried out using Mann-Whitney test. Hair samples were taken from clinically healthy Thoroughbred horses bred in the biogeochemical province of the North Caucasus. It was established that the elemental composition of hair of male horses and mares differed in the content of essential (Co, Fe, Mn, Li, V) and toxic (As, Hg) elements. Reference intervals of the content of the main essential and toxic elements in the hair of mane were calculated, including in accordance with the recommendations of the American Society for Veterinary Clinical Pathology (American Society for Veterinary Clinical Pathology Quality Assurance and Laboratory Standard Guidelines). The data obtained can be used as reference values ​​in assessing diseases and the quality of nutrition of horses.

Key words: horse, thoroughbred horses, elemental status, hair, essential and toxic elements, reference intervals.

References

  1. Cornelis R., Sabbioni E., Van der Venne M.T. Trace element reference values in tissues from inhabitants of the European Community. VII. Review of trace elements in blood, serum and urine of the Belgian population and critical evaluation of their possible use as reference values // Sci Total Environ. 1994. 158(1-3). P. 191-226.
  2. Hamilton E.I., Sabbioni E., Van der Venne M.T. Element reference values in tissues from inhabitants of the European Community. VI. Review of elements in blood, plasma and urine and a critical evaluation ofreference values for the United Kingdom population // Sci Total Environ. 1994. 158(1-3). P. 165-190.
  3. Iyengar G.V. Elemental analysis of biological systems, biological, medical, environmental, compositional and methodological aspects. Boca Raton: CRC Press, 1989. 430 p.
  4. Chyla M.A., Zyrnicki W. Determination of metal concentrations in animal hair by the ICP method.Comparison ofvarious washing procedures // Biological Trace Element Research. 2000. 75. P. 187-194.
  5. Skalny A.V. Evaluation and correction of elemental status of the population as a perspective direction of national healthcare and environmental monitoring // Trace Elements in Medicine. 2018. 19(1). P. 5-13.
  6. Pharmacokinetics of inorganic cobalt and a vitamin B12 supplement in the Thoroughbred horse: Differentiating cobalt abuse from supplementation / L.L. Hillyer, Z. Ridd, S. Fenwick, P. Hincks, S.W. Paine // Equine Veterinary Journal. 2018. May. 50(3). Р. 343-349. doi: 10.1111/evj.12774. Epub 2017 Nov 12.
  7. Spontaneous vascular mineralization in the brain of horses / T. Yanai, T. Masegi, K. Ishikawa, H. Sakai, T. Iwasaki, Y. Moritomo, N. Goto// Journal of Veterinary Medical Science. 1996. Jan. 58(1). Р. 35-40.
  8. Neustädter L.T., Kamphues J., Ratert C.J. Influences of different dietary contents of macrominerals on the availability of trace elements in horses // AnimPhysiolAnimNutr (Berl). 2018. Apr. 102(2). e633-e640. doi: 10.1111/jpn.12805.
  9. Dunnett M., Lees P. Trace element, toxin and drug elimination in hair with particular reference to the horse // Research in Veterinary Science. 2003. No. 75. Р. 89-101.
  10. Nutrition consultation in horses by aid of feed, blood and hair analysis. / W.L. Sippel, J. Flowers, J. O’Farrell, W. Thomas, J. Powers // Proceedings of the American Association of Equine Practitioners. 1964. No. 10. Р. 139-152.
  11. Wysocki A.A., Klett R. Hair as an indicator of the calcium and phosphorus status of ponies // Journal of Animal Science. 1971. № 32. Р. 74-78.
  12. Cape L., Hintz H.F. Influence of month, colour, age, corticosteroids and dietary molybdenum on mineralconcentration of equine hair // American Journal of Veterinary Research. 1982. No. 43. Р. 1132-1136.
  13. Wichert B., Frank T., Kienzle E. Zinc, copper and selenium status of horses in Bavaria // Journal of Nutrition. 2002. No. 132. Р. 1776-1777.
  14. Mihajlovic M. Selenium toxicity in domestic animals// Glas, SrpskaAkademijaNaukaiUmetnosti.OdeljenjeMedicinskihNauka. 1992. No. 42. Р. 131-144.
  15. Davis T.Z., Stegelmeier B.L., Hall J.O. Analysis in horse hair as a means of evaluating seleniumtoxicosesand long-term exposures // Journal of Agricultural and Food Chemistry. 2014. No. 62(30). Р. 7393-7397. doi:10.1021/jf500861p.
  16. Chronic selenosis in horses fed locally produced alfalfa hay / S.T. Witte, L.A. Will, C.R. Olsen, J.A. Kinker, P. Miller-Graber // Journal of the American Veterinary Medicine Association. 1993. No. 202. Р. 406-409.
  17. The detrimental effects of lead on human and animal health / Mohammed AbdulrazzaqAssi, Mohd Noor MohdHezmee, Abd Wahid Haron, MohdYusofMohdSabri, Mohd Ali Rajion // Vet World. 2016. Jun. 9(6). Р. 660-671.
  18. Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age / R. Asano, K. Suzuki, T. Otsuka, M. Otsuka, H. Sakurai // Journal of Veterinary Medical Science. 2002 Jul. 64(7). Р. 607-610.
  19. The content of essential and toxic elements in the hair of the mane of the trotter horses depending ontheir speed / V. Kalashnikov,A. Zajcev, M. Atroshchenko, S. Miroshnikov, A. Frolov, O. Zav’-yalov, L, Kalinkova T. Kalashnikova // Environmental Science and Pollution Research. 2018. May 24. Vol. 25. Issue 22. P. 21961-21967.doi: 10.1007/s11356-018-2334-2.
  20. Madejón P., Domínguez M.T., Murillo J.M. Evaluation of pastures for horses grazing on soils polluted by trace elements // Ecotoxicology. 2009 May. 18(4). Р. 417-428. doi: 10.1007/s10646-009-0296-3.
  21. Shao S., Zheng B. The biogeochemistry of selenium in Sunan grassland, Gansu, Northwest China, casts doubt on the belief that Marco Polo reported selenosis for the first time in history // Environ Geochem Health. 2008. Aug. 30(4). Р. 307-314. doi: 10.1007/s10653-008-9166-9.
  22. Occupational lead poisoning, animal deaths, and environmental contamination at a scrap smelter / R.J. Levine, R.M. Moore, G.D. Maclaren, W.F. Barthel, P.J. Landrigan // American Journal of Public Health. 1976. No. 66. Р. 548-552.
  23. Ward N.I., Savage J.M. Elemental status of grazing animals located adjacent to the London Orbital (M25) motorway // The Science of the Total Environment. 1994. No. 146. Р. 185-189.
  24. Wells L.A., Leroy R., Ralston S.L. Mineral intake and hair analysis of horses in Arizona // Journal of Equine Veterinary Science. 1990. No. 10. Р. 412-416.
  25. Madejón P., Domínguez M.T., Murillo J.M. Pasture composition in a trace element-contaminated area: the particular case of Fe and Cd for grazing horses // Environmental Monitoring and Assessment. 2012. Apr. 184(4). Р. 2031-2043. doi: 10.1007/s10661-011-2097-4.
  26. González-Muñoz M.J., Peña A., Meseguer I. Monitoring heavy metal contents in food and hair in a sample of young Spanish subjects // Food and Chemical Toxicology. 2008. No. 46(9). Р. 3048-3052. doi:10.1016/j.fct.2008.06.004.
  27. Smith K.M., Dagleish M.P., Abrahams P.W. The intake of lead and associated metals by sheep grazing mining-contaminated floodplain pastures in mid-Wales, UK: II. Metal concentrations in blood and wool // Science of the Total Environment. 2010. No. 408(5). Р. 1035-1042. doi:10.1016/j.scitotenv.2009.10.023.
  28. Biological application of laser induced breakdown spectroscopy technique for determination of trace elements in hair / E.M. Emara, H. Imam, M.A. Hassan, S.H. Elnaby // Talanta. 2013. Dec 15. 117. Р. 176-183. doi: 10.1016/j.talanta.2013.08.043. Epub 2013 Sep 7.
  29. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics / K.R. Friedrichs, K.E. Harr, K.P. Freeman, B. Szladovits, R.M. Walton, K.F. Barnhart, Blanco-Chavez // J Vet ClinPathol. 2012. No. 41. Р. 441-453.

30.Development of a method for horsehair sampling for research of elemental composition / S.A. Miro-shnikov, O.A. Zavyalov, A.N. Frolov, M.Ya. Kurilkina, V.V. Kalashnikov, A.M. Zaytsev, M.M. Atrosh-chenko, F.Kh. Sirazetdinov // Animal husbandry and fodder production. No. 101(4). P. 95-101.

  1. National Research council Mineral tolerance of animals. Washington: National Academy Press, 2005. 233р.
  2. The theory of reference values: an unfinished symphony / G. Siest, J. Henny, R. Grasbeck, P. Wilding,C. Petitclerc, J.M. Queralto// Clinical Chemistry and Laboratory Medicine. 2013. No. 51. Р. 47-64. doi: 10.1515/cclm-2012-0682.
  3. Establishment and Use of Reference Values in editors / G.L. Horowitz, C.A. Burtis, E.R. Ash-wood, D.E. Bruns // Tietz textbook of Clinical Chemistry and Molecular Diagnostics. Fifth Edition ed. St. 2012. Р. 95-118. http://dx.doi.org/10.1016/b978-1-4160-6164-9.00005-6.
  4. Center for Biotic Medicine [Electronic resource] url: http://en.microelements.ru/ (reference date 01.02.2019).
  5. National Research Council. Nutrient Requirements of Horses: Sixth Revised Edition. Washington. DC: The National Academies Press, 2007. 324р. https://doi.org/10.17226/11653.
  6. Anke M., Kosla T., Groppel B. The cadmium status of horses from central Europe depending on breed, sex age and living area // Archiv for Tierernahrung. 1989. No. 39. Р. 657-683.
  7. Twenty-eight element concentrations in mane hair samples of adult riding horses determined by particle-induced X-ray emission / K. Asano, K. Suzuki, M. Chiba, K. Sera, R. Asano, T. Sakai // Biol Trace Elem Res. 2005. Nov. 107(2). Р. 135-140.
  8. Content of mineral elements in milk and hair of cows from organic farms / M. Gabryszuk, K. Sloniewski, E. Metera, T. Sakowski // Journal of Elementology. 2010. 15. Р. 259-267.
  9. Jadwiga Topczewska. Effects of seasons on the concentration of selected trace elements in horse hair // Journal of Central European Agriculture. 2012. No. 13(14). Р. 671-680.
  10. Engelhard C. Inductively coupled plasma mass spectrometry: recent trends and developments // Analytical and Bioanalytical Chemistry. 2011. Jan. 399(1). Р. 213-219.
  11. Rodushkin I., Engström E., Baxter D.C. Review Isotopic analyses by ICP-MS in clinical samples // Analytical and Bioanalytical Chemistry. 2013. Mar. 405(9). Р. 2785-2797.
  12. Combs D.K. Hair analysis as an indicator of mineral status of livestock // Journal of Animal Science. 1987. No. 65. Р. 1753-1758.
  13. Cape L., Hintz H.F. Influence of month, color, age, corticosteroids, and dietary molybdenum on mineral concentration of equine hair // American Journal of Veterinary Research. 1982. No. 43. Р. 1132-1136.
  14. Hair concentrations of calcium, iron, and zinc in pregnant women and effects of supplementation / P.L. Leung, H.M. Huang, D.Z. Sun, M.G. Zhu // Biological trace element research. 1999. No. 6. Р. 1222-1229.

    Kalashnikov Valery Vasilyevich, doctor of agricultural sciences, member of RAS, Scientific Director FSBSI «All-Russian Research Institute of Horse Breeding», Russia, tel.: 8(4912)24-02-65, e-mail: vniik08@mail.ru

    Zaytsev Alexander Mikhaylovich,candidate of agricultural sciences, director FSBSI «All-Russian Research Institute of Horse Breeding», Russia, tel.: 8(4912)24-02-65, e-mail: amzaitceff@mail.ru

    Atroshchenko Mikhail Mikhaylovich,candidate of agricultural sciences, FSBSI «All-Russian Research Institute of Horse Breeding», Russia, tel.: 8(4912)24-02-65,e-mail:atromiks-77@mail.ru

    Miroshnikov Sergey Aleksandrovich, doctor of biological sciences, corresponding member of the Russian Academy of Sciences, director FSBSI «Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences» 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)43-46-41; FSBEI HE «Orenburg State University», 460018, Russia, Orenburg, pr. Pobedy, 13, e-mail: vniims.or@mail.ru

    Zavyalov Oleg Aleksandrovich, candidate of agricultural sciences, Department for Beef Cattle Technology and Beef Production FSBSI «Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences», 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)43-46-78, e-mail: oleg-zavyalov83@mail.ru

    Frolov Alexey Nikolaevich, candidate of agricultural sciences, Department for Beef Cattle Technology and Beef Production FSBSI «Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences», 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)43-46-78, e-mail: forleh@mail.ru

    Kurilkina Marina Yakovlevna, candidate of biological sciences, Testing Center FSBSI «Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences», 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)77-39-97, e-mail: icvniims@mаil.ru

Download