Sleptsov II, Dodokhov VV, Pavlova NI, Kayumov FG.

DOI: 10.33284/2658-3135-102-2-60

UDC 636.082.11:636.22/.28.082.13(571.56)

Polimorphism of 15 DNA microsatellite loci of Kalmyk cattle and aboriginal Yakut cattle bred

on the territory of the Republic of Sakha (Yakutia)

I I  Sleptsov1, V V Dodokhov1, N I  Pavlova1, F G  Kayumov2

1 Yakutia State Agricultural Academy (Yakutsk, Russia)

2 Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Summary. This article presents polymorphism of cattle microsatellite markers from Bostaurusturano-mongolicus subgroup: aboriginal Yakut cattle and Kalmyk breed, bred on the territories of the Republic of Sakha (Yakutia). DNA was extracted from whole blood of jugular vein. Genotyping was conducted with COrDISCattle kit according to 15 microsatellite loci (TGLA53, TGLA126, TGLA122, TGLA227, ILST006, ETH3, ETH10, ETH225, BM1818, BM1824, BM2113, CSSM66, CSRM60, SPS115, INRA23). Totally, there are 132 alleles, from them 122 alleles are in Kalmyk breed of cattle, aboriginal Yakut cattle – 68 alleles. In average, 4.53 alleles for a loci in aboriginal Yakut cattle, 8.13 – in Kalmyk breed were revealed. Value of the observed heterozygosis ranged from 0,333 in loci BM1824 to 0,767 (ILSTS6) in aboriginal Yakut cattle. In four loci TGLA227, TGLA122, ETH225, BM2113 value of expected heterozygosis was higher than observed. In cattle of Kalmyk breed the value of observed heterozygosis ranged from 0,667 (ETH10, BM1824, ETH225) to 0.900 in loci ILSTS6. The number of private alleles in aboriginal Yakut cattle was 10: TGLA227 (77), TGLA126 (119), ETH225 (154, 188), TGLA53 (158, 188), CSRM60 (106, 108), ETH10 (215, 225). Frequency ranged from 0,017 in loci TGLA53 (188) to 0,233 in loci ETH225 (154). The fixation index is -0,083 for aboriginal Yakut cattle and -0,007 for Kalmyk breed. Nei’s genetic distance is 0,442, by Fst – 0,0088.

Key words: Yakut cattle, Kalmyk breed, genetic diversity, heterozygosis, genetic distance, polymorphism.

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    Sleptsov Ivan Ivanovich, Cand. Sci. (Econ.), rector, Yakutia State Agricultural Academy, 677007, Yakutsk, Russia, Sergelyahskoe highway 3 km, 3, tel.: 89142735550, e-mail: saas2005@mail.ru

    Dodokhov Vladimir Vladimirovich, Cand. Sci. (Biol.),SeniorLectureroftheDepartmentofTraditionalBranchesoftheNorth, Yakutia State Agricultural Academy, 677007, Yakutsk, Russia,Sergelyahskoe highway 3 km, 3,tel.: 89644220278, e-mail:dodoxv@mail.ru

    Pavlova Nadezhda Ivanovna, Cand. Sci. (Biol.), Researcher of ResearchPart, Yakutia State Agricultural Academy,677007, Yakutsk, Russia,Sergelyahskoehighway 3 km, 3,e-mail: nadezdaiv@inbox.ru

    Kayumov Foat Galimovich, Dr. Sci. (Agr.), Professor, Head of the Scientific Direction, Head of the Laboratory of New Breeds and Types of Beef Cattle Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St.;  tel.: 8(3532)43-46-76, cell phone 8-987-341-75-80, е-mail: vniims.or@mail.ru, nazkalms@mail.ru

Received: 23 May 2019; Accepted: 17 June 2019; Published: 28 June 2019

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