E A Azhmuldinov, M G Titov, M A Kizaev, I A Babicheva, N V Soboleva
Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0006)
The role of silver nanoparticles in technological stresses of bulls
E A Azhmuldinov1, M G Titov1, M A Kizaev1, I A Babicheva2, N V Soboleva2
1Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)
2Orenburg State Agrarian University (Orenburg, Russia)
Summary. Researches of the effect of silver nanoparticles on animal organism at technological stresses were carried out on Black Spotted bulls in JSC Agrofirm «Nur» in Sterlibashevsky district of the Republic of Bashkortostan. Three groups with 15 heads in each were formed: one control and two experimental groups. Experimental animals were intramuscularly injected with an emulsion (pH – 9.5, redox potential Eh=-450 mV) with silver nanoparticles in doses: group I – 0.01 mg/kg body weight and II – 0.05 mg/kgbody weight, once a day for seven days prior to the action of stress factors (8 months – rearrangement, 18 months – transportation to meat processing plant and pre-slaughtering keeping).
An emulsion with silver nanoparticles in doses of 0.01 and 0.05 mg/kg helps to reduce body stress when exposed to a stress factor. It was reflected in the indicators of serum enzymatic activity of the experimental groups, it increased after the exposure of stress factors, but to a much lesser extent than that of the control animals. After transport stress, the activity of aminotransferases in relation to control animals was lower: gamma-glutamyltransferase (GGT) – by 11.1 and 7.4%; aspartate aminotransferase (AST) – by 6.6 and5.8%; alanine aminotransferase (ALT) – by 31.4 and 29.9%. During the experiment, bulls of groups I and II advanced in live weight at the age of 18 months by 1.9 and 1.7% compared to control animals, and by average daily weight gain – by 4.7 and 3.2%. They occupied a more favorable position in terms of meat productivity: by weight of fresh carcass – by 4.2 and 3.4%, slaughter mass – by 4.2 and 3.3% and slaughter yield – by 0.3 and 0.2% more than analogues of the basic variant. The highest result was achieved after intramuscular administration of an emulsion with silver nanoparticles at a dose of 0.01 mg/kg.
Key words: bulls, silver nanoparticles, gamma-glutamyltransferase, alanine aminotransferase, aspartate aminotransferase, transportation, live weight, stress, beef productivity.
- Azhmuldinov EA, Titov MG. A comparative assessment of adaptability of bulls of different breeds. (Conference proceedigs) Ways to intensify the production and processing of agricultural products in modern conditions: international materials of scientific-practical conf.: in 2 parts. edited by Khramova VN. Volgograd, 2012. Part 1. P. 54-56.
- Popov VV, Levakhin VI, Karabanov EP, Titov MG. Weight growth of young cattle of different breeds and productivity directions in an industrial complex conditions. Herald of Beef Cattle Breeding. 2009;62(4):60-61.
- VladimirovYuA, Archakov AI. Lipid peroxidation in biological membranes. Moscow: Nauka; 1972. 252 p.
- Nikulin VN, Babicheva IA, Gerasimenko VV, AzhmuldinovYeA, Kizaev MA.Titov MG. Effect of chromium nanoparticles on live weight loss of black-spotted steers exposed to technological stresses. Izvestia Orenburg State Agrarian University. 2018;6(74):208-210.
- Azhmuldinov EA, Kizaev MA, Titov MG, Babicheva IA. Influence of various stress factor on the organism of farm animals (review). Animal Husbandry and Fodder Production. 2018;101(2):79-89.
- Meerson FZ. Adaptation, stress and prevention. Moscow: Nauka; 1981. 279 p.
- Kalashnikov AP, et al.Standards and diets of farm animals: Ref. book. 3rd ed., rework. and add. Moscow: Agropromizdat; 2003: 456 p.
- Levakhin VI, Salo AV, Korovin AS, Popov VV, Schwindt VI, Rogachev BG, LevakhinYuI, Sirazetdinov FH, Chernykh AP, Akhmetova FF, IsyangulovaGKh, Zhuravlev NV, Titov MG. A method to reduce production losses of young cattle during transportation and pre-slaughter keeping: Pat. 2396948 Russian Federation. Application 27.02.09; publ. 20.08.10, Bull. Number 23.
- DvornikovVM. The stabilizer of an aqueous solution and water-containing raw materials with spontaneously changing redox properties: Pat. 2234945Russian Federation.Application 15.10.02; publ. 27.08.04, Bull. Number 24.
- Titov MG. Use of Zigbir preparation for reduction of production losses of bulls in conditions of industrial keeping technology. Herald of Beef Cattle Breeding. 2017;4(100):124-128.
- Abou El-Nour KMM, Eftaiha A, Al-Warthan A, Ammar RAA. Synthesis and applications of silver nanoparticles. Arab. J. Chem. 2010;3(3):135-140. doi: https://doi.org/10.1016/j.arabjc.2010.04.008
- Aklakur M, Rather MA, Kumar N. Nanodelivery: an emerging avenue for nutraceuticals and drug delivery. Crit Rev Food SciNutr. 2016. 56(14):2352-2361. doi: https://doi.org/10.1080/10408398.2013.839543
- AL-Hashem FH, Shatoor A, Sakr HF, et al. Co-administration of vitamins E and C protects against stress-induced hepatorenal oxidative damage and effectively improves lipid profile at both low and high altitude. African J. of Biotechn. 2012;11(45):10416-10423. doi: https://doi.org/10.5897/AJB12.058
- Beck I, Hotowy A, Sawosz E, Grodzik M, Wierzbicki M, Kutwin M, Jaworski S, Chwalibog A. Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos. Arch AnimNutr. 2015;69:57-68. doi: https://doi.org/10.1080/1745039X.2014.992179
- Cao G, Wang Y. Nanostructures and nanomaterials: synthesis, properties, and applications. 2nd ed. World Scientific Series in Nanoscience and Nanotechnlogy. Vol. 2. New York: World Scientific Publishing, 2011. 596 p. doi: https://doi.org/10.1142/7885
- del Bello B, Paolicchi A, Comporti M, et al. Hydrogen peroxide produced during gamma-glutamyl activity is involved in prevention of apoptosis and maintenance of cell proliferation in U937 cells. Faseb. J. 1999;13:69-79.
- Earley B, Murray M, Prendiville DJ. Effect of road transport for up to 24 hours followed by twenty-four hour recovery on live weight and physiological responses of bulls. BMC Vet Res. 2010;6:Article number 38. 13 p. doi: https://doi.org/10.1186/1746-6148-6-38
- Elechiguerra JL, Burt JL, Morones JR, Camacho-Bragado A, Gao X, Lara HH, Yacaman MJ. Interaction of silver nanoparticles with HIV-1. J. Nanobiotechnol. 2005;3:Article number 6. 10 p. doi: https://doi.org/10.1186/1477-3155-3-6
- Kolesnichenko LS, Kulinski VI, Ias'ko MV, et al. The effect of emotional-painful stress, hypoxia, and adaptation to it on the activity of enzymes for metabolizing glutathione and concentration of glutathione in rat organs. Vopr. med. khim. 1994;40(5):10-12.
- Lu Z, Rong K, Li J, Yang H, Chen R. Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria. J. Mater. Sci. Mater. Med. 2013;24(6):1465-1471. doi: https://doi.org/10.1007/s10856-013-4894-5
- Paolicchi A, Tongiani R, Tonarelli P, Comporti M, Pompella A. Gamma-glutamyltranspeptidase-dependent lipid peroxidation in isolated hepatocytes and HepG2 hepatoma cells. Free. Rad. Biol. Med. 1997;22(5):853-860. doi: https://doi.org/10.1016/S0891-5849(96)00422-4
- Saini P, Saha SK, Roy P, Chowdhury P, Sinha Babu SP. Evidence of reactive oxygen species (ROS) mediated apoptosis in Setariacervi induced by green silver nanoparticles from Acacia auriculiformis at a very low dose. Exp. Parasitol. 2016;160:39-48. doi: https://doi.org/10.1016/j.exppara.2015.11.004
- Samuel U, Guggenbichler JP. Prevention of catheter-related infections: the potential of a new nano-silver impregnated catheter. Int J Antimicrob Agents. 2004; 23(S1):75-78. doi: https://doi.org/10.1016/j.ijantimicag.2003.12.004
- Sarkar B, Kumar M, Verma S, Rhathore RM. Effect of dietary nanosilver on gut proteases and general performance in zebrafish (Daniorerio). Int J Aqut Biol. 2015;3(2):60-67.
- Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli.Nanomedicine: NMB. 2007;3(2):168-171. doi: https://doi.org/10.1016/j.nano.2007.02.001
- Sun RWY, Chen R, Chung NPY, Ho CM, Lin CLS, Che CM. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chem. Commun. 2005; 40:5059-5061. doi: 10.1039/b510984a
- Tarrant PV. Transportation of cattle by road. Appl Animal Beh Sci. 1990;28(1-2):153-170. doi: https://doi.org/10.1016/0168-1591(90)90051-E
- Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int. J. Mol. Sci. 2016;17(9):1534 34 p. doi: https://doi.org/10.3390/ijms17091534
- Zhang XF, Park JH, Choi YJ, Kang MH, et al. Silver nanoparticles cause complications in pregnant mice. Int. J. Nanomed. 2015;10(1):7057-7071. doi: https://doi.org/10.2147/IJN.S95694
- Zhu L, Guo D, Sun L, Huang Z, Zhang X, Ma W, Wu J, Xiao L, Zhao Y, Gu N. Activation of autophagy by elevated reactive oxygen species rather than released silver ions promotes cytotoxicity of polyvinylpyrrolidone-coated silver nanoparticles in hematopoietic cells. Nanoscale. 2017;9:5489-5498. doi: 10.1039/C6NR08188F
Azhmuldinov Elemes Azhmuldinovich, Dr. Sci (Agr.), Professor, ChiefResearcher,Department for Beef Cattle Technology and Beef Production, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78
Titow Maxim Gennadevich, Cand. Sci(Agr.), Senior Researcher, Department for Beef Cattle Technology and Beef Production, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78, e-mail: email@example.com
Kizaev Mikhail Anatolevich, Cand. Sci (Agr.),ScientificSecretary, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78,e-mail: firstname.lastname@example.org
Babicheva Irina Andreevna, Dr. Sci. (Biol.), Professor, Head of Department of Chemistry,OrenburgStateAgrarianUniversity, 460014, Orenburg, Russia, 18, Chelyuskintsev St.,tel./fax: 8(3532)77-52-30, e-mail: email@example.com
Soboleva Natalya Vladimirovna, Cand. Sci (Agr.),Assoc. Prof.Department of Livestock Production and Processing Technology, OrenburgStateAgrarianUniversity, 460014, Orenburg, Russia, 18, Chelyuskintsev St.,tel.:89033676715, e-mail:firstname.lastname@example.org
Received: 21 August 2019; Accepted: 16 September 2019; Published: 30 September 2019Download