A V Kharlamov, A N Frolov, O A Zavyalov, E A Tyapugin
Research was carried out according the plan of reasearch scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0006)
The effect of gene polymorphism of growth differentiation factor 5
on morphological and biochemical parameters of blood
A V Kharlamov, A N Frolov, O A Zavyalov, E A TyapuginFederal Research Center for Biological System and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)
Summary. Studies were conducted on calves of the Kalmyk breed (n=182), age 12-14 months, body weight 343.7±6.4 kg. Blood samples were taken to identify one nucleotide polymorphism of growth differentiation factor 5 (T586C in exon 1).
DNA samples were isolated from whole blood using the «DIAtomtmDNAPrep 200» reagent kit (IsoGeneLab, Moscow). For the polymerase chain reaction, the GenePaktmPCRCore kit («IsoGeneLab», Moscow) and the EncycloPCRkit kit («Evrogen», Moscow) were used. Primers are synthesized in NPF «Litekh» (Russia).
The incidence of TT alleles in sample was established, it amounted to 48.9%, ТС– 46.7 and СС– 4.4%, χ2 test – 4.94.
At the second stage of research, in order to study the effect of polymorphism in GDF5 gene on morphological and biochemical parameters of blood, 8 animals were selected from each identified group of animals.
Morphological parameters were determined using an automatic hematological analyzer model URIT-2900 Vet Plus, a biochemical blood test was performed using an automatic biochemical analyzer CS-T240. 35 blood counts were studied.
It was found that blood of bulls with CC genotype contained 5.2% more crude protein, cholesterol – 15.5%, transferases: ALT – 6.9% and ɣ-GT – 20.2%, at a lower percentage monocytes (MID) – by 5.6 % and the average platelet volume (MPV) – by 9.2% compared with the TT genotype.
It was concluded that the effect of polymorphism on growth gene for differentiation factor 5 on 6 blood indices. The further research need with increase of animal group with homozygous genotype C in this gene.
Key words: cattle, bulls, gene, GDF5, SNP, blood.
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Kharlamov Anatoly Vasilyevich, Dr. Sci (Agr.), Professor, Head of Department for Beef Cattle Technology and Beef Production, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., 29, tel.: 8(3532)43-46-78, e-mail: firstname.lastname@example.org
Frolov Alexey Nikolaevich, Cand. Sci (Agr.), Senior Researcher, Department for Beef Cattle Technology and Beef Production, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78, e-mail: email@example.com
Zavyalov Oleg Aleksandrovich, Cand. Sci (Agr.), Senior Researcher, Department for Beef Cattle Technology and Beef Production, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences», 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78, e-mail: firstname.lastname@example.org
Tyapugin Yevgeny Aleksandrovich, Dr. Sci (Biol.),Academician of Russian Academy of Sciences, ChiefResearcherLaboratoryofmoleculargeneticresearchandmetallomicsinanimalhusbandry, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)43-46-78, e-mail: email@example.com
Received: 5 September 2019; Accepted: 16 September 2019; Published: 30 September 2019