N I Voskobulova, A S Vereshagina, R Sh Uraskulov, M Ya Kurilkina
Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0004)
Amino acid composition and biological value of pea protein depending on cultivation techniques
N I Voskobulova, A S Vereshagina, R Sh Uraskulov, M Ya Kurilkina
Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)
Summary. The article presents the results of studies on the effect of seeding rates on the content of protein and amino acids in pea grains.
In the steppe zone of the Orenburg region field experiments with two varieties of peas Samarius and Flagman 12 were conducted according to the method of BA. Dospekhov. The content of crude protein in pea grain and its amino acid composition in variants with seeding rates of 0.6; 0.8; 1.0; 1.2; 1.4 million seeds per 1 ha were studied.
The biological value of proteins was determined according to the method of amino acid scoring. The amino acid composition of pea grain protein was compared with the amino acid parameters of a standard («ideal») protein (chicken protein).
In the course of research, it was found that the amount and quality of protein in pea seeds depends on the growing conditions, genetic characteristics and cultivation technology. Dry hot weather during the growing season and an increase in seeding rates increased the content of crude protein in pea seeds.
Under the influence of the seeding rate, the amino acid composition of pea protein changed. Increasing seeding rate from 0.6 to 1.4 million of germinating seeds per 1 ha, the content of main essential amino acids of Samarius variety decreased, and in variety Flagman 12 it increased. The amino acid content of leucine + isoleucine and lysine in pea protein was by 98.2-145.4 and 72.7-101.8% in accordance with the recommended standards of FAO/WHO Food Committee. The limiting amino acid in pea seeds was methionine. The research results make it possible to recommend peas as a component of compound feeds as a source of essential amino acids.
Key words: peas, varieties, Samarius, Flagman 12, protein, amino acids, Orenburg region.
- Boyarintseva GG, Shatskikh EV, Gafarov ShS. Workshop on breeding farm animals: a textbook. Yekaterinburg: Ural State Agricultural Academy; 2005. 188 p.
- Golushko VM, Golushko AV. Protein and amino acids of feed for pigs. Zootechnical science of Belarus. 2015;50(1):220-232.
- Shpaar D, Ellmer F, Postnikov A, Taranuho G, et al. Legumes. Shpaara D, edited. Minsk: FUAinform; 2000. 264 p.
- Zotikov VI.Leguminous and groats crops is an actual direction of improvement of quality of production. Legumes and groat crops. 2017;3(23):23-28.
- Isaichev VA, Andreev NN, Mudarisov FA. The influence of growth regulators and chelated micronutrient fertilizers on productivity and quality indicators of peas and winter wheat. Bulletin of the Ulyanovsk State Agricultural Academy. 2012;1(17);12-16.
- Poluektov RA, Smolyar EI, Tepleev VV. Topage A.G. Models of production process of agricultural crops. SPb .: Publishing House of SPbGU; 2006. P. 25-31.
- Golushko VM, Roschin VA, Linkevich SA, Golushko AV, Shatskiy MA, Shevtsova EF.Determining available lysine in protein feeds for pigs. Zootechnical science of Belarus. 2018;53(1):208-217. (In Russ)].
- Pigorev IYa, Danilova LV. The influence of seeding rates on the yield and quality of soybean seeds on gray forest soils of the Central Black Earth Region. Bulletin of Kursk State Agricultural Academy. 2009;3:57-59.
- Putyatin YuV, Markevich DV, Tavrykina OM. Comparative analysis of the composition of essential amino acids in the production of forage crops. Soil Science and Agrochemistry. 2013;2(51):229-236.
- Rozhkov AA. The influence of sowing methods and seeding rates on changes in grain quality indicators of spring triticale plants. Bulletin of Kursk State Agricultural Academy. 2014. No. 1. S. 37-40.
- Savchuk SV, Sakovtseva TV. Calculation methods for determining the biological value of a protein. Biological chemistry for bachelors in biology: a textbook. Moscow: Publishing House of the Russian State Authentic Agricultural Academy named after K.A. Timiryazev, 2015. P. 22.
- Katyuk AI, Zubov AE, Maystrenko OA, Budilov AP, Voskobulova NI. Varieties of pea breeding Samara Research Institute of Agriculture for food and feed purposes. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2015;17(4-3):505-509.
- De Ron AM, Sparvoli F, Pueyo JJ, Bazile D. Editorial: protein grops: food and feed for the future. Frontiers in Plant science. 2017;8:105. doi: https://doi.org/10.3389/fpls.2017.00105
- Amino acids in animal nutrition. 2nd. ed. edited by D'Mello JPF. Cambridge: CABI Publishing. 2003; 513 p.
- Grain Legumes. Series: Handbook on Plant Breeding. Vol. 10. De Ron AM, editor. New York, NY; Dordrecht; Heidelberg; London: Springer; 2015. 438 p. doi: https://doi.org/10.1007/978-1-4939-2797-5
- Galili G, Amir R, Hoefgen R, Hesse H. Improving the levels of essential amino acids and sulfur metabolites in plant. Biol. Chem. 2005;386(9):817-831. doi:https://doi.org/10.1515/BC.2005.097
- Galili G, Amir R, Fernie AR. The regulation of essential amino acid synthesis and accumulation in plants. Annual Review of Plant Biology. 2016;67:153-178. doi: https://doi.org/10.1146/annurev-arplant-043015-112213
- Müntz K, Christov V, Saalbach G, Saalbach I, Waddell D, Pickardt T, Schieder O, Wustenhagen T. Genetic engineering for high methionine grain legumes. Nahrung. 1998;42(3-4):125-127.
- Toride Y. Lysine and other amino acids for feed: production and contribution to protein utilization in animal feeding. Protein sources for the animal feed industry: Expert Consultation and Workshop; 2002 29 April-3 May; Bangkok. Rome: Food and Agriculture Organization of the United Nation; 2004. P. 161-165.
Voskobulova Nadezhda Ivanovna, Cand. Sci. (Agr.), Leading Researcher, Head of Department Fodder Technologies, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460051, Orenburg, Russia, 27/1Gagarin Avenue, tel.: 8(3532)43-46-90, cell phone: 8-919-864-34-40, e-mail: firstname.lastname@example.org
Vereshchagina Antonina Sergeevna, Cand. Sci (Agr.), Senior Researcher, DepartmentFodderTechnologies, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460051, Orenburg, Russia, 27/1Gagarin Avenue, tel.: 8(3532)43-46-23, cell phone: 8-919-853-17-26
Uraskulov Ruslan Shamilyevich, Senior Researcher, DepartmentFodderTechnologies, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460051, Orenburg, Russia, 27/1Gagarin Avenue, tel.: 8(3532)43-46-23
Kurilkina Marina Yakovlevna, Cand. Sci (Biol.), Researcher of the Testing Center CCU, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St., tel.: 8(3532)77-39-97, e-mail: icvniims@mаil.ru
Received: 12 September 2019; Accepted: 16 September 2019; Published: 30 September 2019