Muselin F, Dumitrescu E, Doma AO, Cristina RT, Gârban Z, Alexandru BV

DOI: 10.33284/2658-3135-102-4-61

UDC 599.323.4:577.17



The reported study was financed by the project: Development of research infrastructure, education and services in the fields of veterinary medicine and innovative technologies for RO 05.Code SMIS-CSNR 2669. Special thanks for their support to Mr. Giorgio Gherardi and Mrs. Daniela Csoric from GG Diagnostiaca.

The status of some bioelements in rats with oxidative stress induced by cisplatinum and aluminium

Florin Muselin1,2, Eugenia Dumitrescu1, Alexandru O Doma1,Romeo T Cristina 1, Zeno Gârban2, Alexandru B Vițălaru3

1Banat`s University of Agricultural Sciences and Veterinary Medicine “King of Romania Michel I”

from Timisoara (Timisoara, Romania)

2Romanian Academy-Branch Timisoara (Timişoara, România)

3University of Agronomic Sciences and Veterinary Medicine of Bucharest (București, Romania)

Summary. The paper presents data regarding the status of some important bioelements that act as co-factors for different enzymes in rats with induced oxidative stress by the administration of aluminum and cisplatinum. The study was made on thirty-six Wistar albino young rats (three months old) divided randomly in four groups as follows: C – Control, 1 ml of physiologically saline (P.S); E1 – administration of 100 mg/kg bw AlCl3; E2 – administration of 10 mg/kg bw cisplatinum; E3 – administration of 100 mg/kg bw AlCl3 and 10 mg/kg bw cisplatinum. All administrations were via the intraperitoneal route (i.p.), once a week, for a four weeks period. Were measured the oxidative stress enzymes CAT (catalase), SOD (superoxide dismutase), glutathione peroxidase (GPx), Glutathione reductase (GSH-r) and the blood levels of iron, manganese, magnesium, copper, zinc, and selenium. In all experimental groups were observed the significant decrease of CAT, the significant increase of GPx and GSH-r, not significant increase of SOD, not significant fluctuations of manganese and magnesium, a significant decrease of zinc and copper and not significant decrease of selenium. We can conclude that aluminum and cisplatinum can impair the normal status of the main bioelements through induced oxidative stress.

Key words: rats, bioelements, trace elements,oxidative stress, cisplatinum, aluminium.


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Muselin Florin, PhD, DVM, MS, Associate Professor, Department of Toxicology, Faculty of Veterinary Medicine, Banat`s University of Agricultural Sciences and Veterinary Medicine “King Michel I of Romania” from Timisoara, 300645, Timisoara, Romania,Calea Aradului 119; Romanian Academy-Branch Timisoara, 300223, Timişoara, România, Bv. Mihai Viteazu 24,е-mail:

Dumitrescu Eugenia, PhD, DVM, Associate Professor,Departments of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, Banat`s University of Agricultural Sciences and Veterinary Medicine “King Michel I of Romania” from Timisoara, 300645, Timisoara, Romania,Calea Aradului 119

Doma Alexandru Octavian, student in Faculty of Veterinary Medicine, Banat`s University of Agricultural Sciences and Veterinary Medicine “King Michel I of Romania” from Timisoara, 300645, Timisoara, Romania,Calea Aradului 119

Cristina Romeo Teodor, DVM, Professor, Head of Department of Toxicology, Faculty of Veterinary Medicine, Banat`s University of Agricultural Sciences and Veterinary Medicine “King Michel I of Romania” from Timisoara, 300645, Timisoara, Romania,Calea Aradului 119 Gârban Zeno, PhD, Professor, Corresponding Member of the Romanian Academy, Founding member of „Working Group for Metal Research in Biological Systems”, Romanian Academy-Branch Timisoara, 300223, Timişoara, România, Bv. Mihai Viteazu 24

Vitalaru Alexandru Bogdan, PhD, DVM, Associate Professor, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 050097 București, Romania, Splaiul Independenței 105

Received: 25 November 2019; Accepted: 16 December 2019;Published: 31 December 2019