Nemereshina ON, Gusev NF, Trubnikov VV

DOI: 10.33284/2658-3135-102-4-69

UDC 582.998.2(470.56)

Peculiarities of elemental composition of milfoil under conditions of technogenic pollution

Olga N Nemereshina1, Nikolay F Gusev2, Victor V Trubnikov3

1Orenburg State Medical University (Orenburg, Russia)

2Orenburg State Agrarian University(Orenburg, Russia)

3Branch of the Russian State University of Oil and Gas (National Research Institute) named after

IM Gubkina in Orenburg (Orenburg, Russia)

Summary. Heavy metals are one of the most important technogenic pollutants due to the pronounced ability to cumulate in the surface fertile soil layer and to migrate in food chains.

The article presents the results of determining the elemental composition of medicinal plant materials (grass) of milfoil (Achillea millefolium L.), which grows under various technogenic loads. Milfoil plant samples were collected under the technogenic influence of Orenburg Gas Processing Plant in the territory of sanitary protection zone and various production facilities of the enterprise. The control zone on the territory of the Orenburg district of Orenburg region selected the surroundings of the villages of Kamennozernoe and Nezhinka. The content of Mn, Zn, Fe, Cu, Ba, Sr, Pb, Zr, Ni, Cr, V, Co, Ag, Mo was determined by atomic absorption spectrometry in plant raw materials. It was found that the level of content of the studied heavy metals in raw milfoil growing in the zone of atmospheric emissions of Orenburg gas processing plant significantly exceeds the control indicators. The accumulation of heavy metals in tissues can serve as a factor provoking oxidative stress of plants, lead to a decrease in the productivity of species, and induce the synthesis of components of antioxidant defense systems.

Key words: medicinal plants, feed plant, Achillea millefolium L., heavy metals, plant ecology, environmental pollution, Orenburg region.


  1. Alekseev YuV. Heavy metals in soils and plants. L.: Agropromizdat, 1987;142p.
  2. Golovko T, Garmash E, Skugoreva S. Heavy metals in the environment and plant organisms. Bulletin of Institute of Biology of Komi Scientific Center, Ural Branch of RAS. 2008;7(129):2-7.
  3. Grigoryeva AA, Mironova GE. Аccumulation of heavy metals in soil and in forage grasses of Сentral Yakutia. Vestnik Of North-Eastern Federal University. 2018;1(63):5-12.
  4. Gusev NF, Petrova GV, Nemereshina ON. Medicinal plants of Orenburg region. Orenburg: Publishing House of the OGAU; 2007: 331 p.
  5. Ignatovich LS. Influence of use of component feed additives, manufactured with the use of grass meal of yarrow, on laying hens productivity, quality of the products (eggs) and feed conversion. Far Eastern Agrarian Herald. 2017;2(42):75-81.
  6. Ilyin VB, Syso AI. Trace elements and heavy metals in soils and plants of Novosibirsk region: monograph. Novosibirsk: Publishing House Sib. Dep. RAS; 2001: 229 p.
  7. Korhz L. Enriching diets of layers. Livestock of Russian. 2018;S3:22.
  8. Pavlovskaya NE, Grinblat AI. Active forms of oxygen and apoptosis in wheat and pea. Sel’skokhozyaistvennaya Biologiya[Agricultural Biology]. 2010;1:51-55.
  9. Skalny AV. Trace elements: vitality, health, longevity. Moscow: Publishing House "Pero"; 2019: 295 p.
  10. Tarabrin VP. The physiology of sustainability of woody plants under environmental pollution by heavy metals. Trace elements in the environment. Kiev: Naukova Dumka; 1980:17-19.
  11. Titov AF, Kaznina NM, Talanova VV. Heavy metals and plants. Petrozavodsk: Institute of Biology Karel. scientific Center, 2014. 194 p.
  12. Bonfranceschi BA, Flocco CG, Donati ER. Study of the heavy metal phytoextraction capacity of two forage species growing in an hydroponic environment. Journal of Hazardous Materials. 2009;165(1-3):366-371. doi:
  13. Cobbett CS. Phytochelatins and their roles in heavy metal detoxification. Plant Physiology. 2000;123:825-832. doi:
  14. Cuypers A, Smeets K, Vangronsveld J. Heavy metal stress in plants. Plant stress biology: From genomics to systems biology.Hirt H, editor. Weinheim: Wiley-VCH Verlag; 2009: 161-178.
  15. de la Calle Guntiñas MB, Wysocka I, Quétel C, et al. Proficiency test for heavy metals in feed and food in Europe. TrAC Trends in Analytical Chemistry. 2009;28(4):454-465. doi:
  16. Gratão PL, Alves LR, Lima LW. Heavy metal toxicity and plant productivity: role of metal scavengers. Plant-metal interactions. Srivastava S, Srivastava A, Suprasanna P, editors. Springer: Cham; 2019:49-60. doi:
  17. Haber F, Weiss J. On the catalysis of hydroperoxide. Naturwissenschaften. 1932;20:948-950. doi: 10.1007/BF01504715
  18. Kim HS, Kim YJ, Seo YR. An overview of carcinogenic heavy metal: molecular toxicity mechanism and prevention. Journal of Cancer Prevention. 2015;20(4):232-240.
  19. Sharma I, SharmaA, Pati P, Bhardwaj R. Brassinosteroids reciprocates heavy metals induced oxidative stress in radish by regulating the expression of key antioxidant enzyme genes. Brazilian Archives of Biology and Technology. 2018;61:e18160679. doi:

Nemereshina Olga Nikolaevna, Cand. Sci. (Biol), Associate Professor of the Department of Biochemistry, Orenburg State Medical University, 460000, Orenburg, Russia, 6 Sovetskaya St., tel.: 8(3532)77-61-03, 77-94-08, 8(905)8901330 e-mail:

Gusev Nikolay Fedorovich, Dr. Sci. (Biol.), Professor, Department of Bioecology, Orenburg State Agrarian University, 460014, Orenburg, Russia, 18 Chelyuskintsev St., tel: 8(3532)77-52-30, fax: 8(3532)77-52-30, 72-57-06, 8(912)3523669, e-mail:

Trubnikov Victor Vladimirovich, Cand. Sci. (Tech.), Associate Professor, Branch of the Russian State University of Oil and Gas (National Research Institute) named after IM Gubkin in Orenburg, 460047, Orenburg, Russia, 20 Yunykh Lenintsev St.

Received: 21 November 2019; Accepted: 16 December 2019;Published: 31 December 2019