Atlanderova KN, Duskaev GK, Makaeva AM, Muslyumova DM, Kondrashova KS

DOI: 10.33284/2658-3135-102-4-186

UDC 636.085:577.17


Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0526-2019-0002)

Cattle rumen microbiome after Quercus cortex extract

Kseniya N Atlanderova, Galimzhan K Duskaev, Aina M Makaeva, Dina M Muslymova,

Kristina S Kondrashova

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Summary. The paper presents the results of studies of the effect of Quercus cortex extract in various concentrations on the digestibility of feed dry matter by in situ method after 3 and 6 hours of exposure in rumen. Data analysis showed that the introduction of Quercus cortex extract (3.3 mg / ml) in the diet of young cattle increases digestibility by 2.43% (P≤0.05) and 6.33% (P≤0.01) after 3 and 6 hours, respectively, relative to the control.

Quercus cortex extract had a significant effect on the ratio of gram-negative and gram-positive rumen microflora. The experiment revealed a predominance of Firmicutes largely, 1.7% higher than the control, it was expressed with a change in the proportion of representatives of Clostridia, Bacteroidia and Bacilli classes in the microbiocenosis.

Within the taxa Firmicutes and Proteobacteria, a decrease in the number of bacteria of the classes Clostridia (by 8.8% of the control), Negativicutes (by 3.0% of the control group) was noted. Whereas, within the framework of the taxon Bacteroidetes, an increase in the number of bacteria of Bacilli class by 13.5% of the control was observed, which was mainly not associated with an increase in representatives of Streptococcus (14.4% of the total).

In the experimental group, 665 taxonomic categories were identified, 8 of them were the most predominant, including Streptococcus bovis - 14.5%, Prevotella ruminicola - 3.1%, Butyrivibrio proteoclasticus - 1.5% of the total number of microorganisms.

Key words: cattle, microbiome, Quercus cortex extract, anti-quorum substances, rumen.


  1. Bagirov VA, Duskaev GK, Kazachkova NM, Rakhmatullin ShG, Yausheva EV, Kosyan DB, Makaev ShA, Dusaeva KhB. Addition of Quercus cortex extract to broiler diet changes slaughter indicators and biochemical composition of muscle tissue. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology]. 2018;53(4):799-810. doi: 10.15389/agrobiology.2018.4.799eng

2.Duskaev GK, Drozdova EA, Alyoshina ES, Bezryadina AS. Estimation of impact on the intestinal microflor of birds of substances with antibiotic, probiotic and anti-quorum sensing effects. Vestnik Orenburg State University. 2017;11(211):84-87.

  1. Anantasook N, Wanapat M, Cherdthong A, Gunun P. Effect of tannins and saponins in Samanea saman on rumen environment, milk yield and milk composition in lactating dairy cows. Journal of Animal Physiology and Animal Nutrition. 2015; 99(2):335-344. doi:
  2. Atlanderova K, Makaeva A, Miroshnikov S, Ivanishcheva A. Changes in rumen microbiota of cattle with the simultaneous introduction of iron and copper nanoparticles and quorum sensing suppressants. FEBS Open Bio 9. 2019;Suppl.1: 415-416. doi:
  3. Creevey CJ, Kelly WJ, Henderson G, Leahy SC. Determining the culturability of the rumen bacterial microbiome. Microbial Biotechnology. 2014;7(5):467-479. doi:
  4. Deryabin DG. Tolmacheva AA. Antibacterial and anti-quorum sensing molecular composition derived from Quercus cortex (Oak bark) Extract. Molecules. 2015;20(9):17093-17108. doi:
  5. Duskaev GK, Kazachkova NM, Ushakov AS, Nurzhanov BS, Rysaev AF. The effect of purified Quercus cortex extract on biochemical parameters of organism and productivity of healthy broiler chickens. Vet World. 2018;11(2):235-239. doi: 10.14202/vetworld.2018.235-239
  6. Franzolin R, Wright ADG. Microorganisms in the rumen and reticulum of buffalo (Bubalus bubalis) fed two different feeding systems. BMC research notes. 2016;9:243. doi: 10.1186/s13104-016-2046-y
  7. Gadde U, Kim WH, Oh ST, Lillehoj HS. Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Anim Health Res Rev. 2017;18(1):26-45. doi:
  8. Golder HM, Denman SE, McSweeney C, Wales WJ, Auldist MJ, Wright MM, Marett LC, Greenwood JS, Hannah MC, Celi P, Bramley E, Lean IJ. Effects of partial mixed rations and supplement amounts on milk production and composition, ruminal fermentation, bacterial communities, and ruminal acidosis. J Dairy Sci. 2014;97(9):5763-5785. doi:
  9. Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance Journal of Ayurveda and Integrative Medicine. 2017;8(4):266-275. doi:
  10. Hall AB, Tolonen AC, Xavier RJ. Human genetic variation and the gut microbiome in disease. Nat Rev Genet. 2017;18(11):690-699. doi:
  11. Jami E, White BA, Mizrahi I. Potential role of the bovine rumen microbiome in modulating milk composition and feed efficiency. Plos One. 2014;9(1):e85423. doi:
  12. Kim JE, Lillehoj HS, Hong YH, Kim GB, Lee SH, Lillehoj EP, Bravo DM. Dietary Capsicum and Curcuma longa oleoresins increase intestinal microbiome and necrotic enteritis in three commercial broiler breeds. Res Vet Sci. 2015;102:150-158. doi:
  13. Kim YH., Nagata R, Ohtani N, Ichijo T, Ikuta K, Sato S. Effects of dietary forage and calf starter diet on ruminal pH and bacteria in Holstein calves during weaning transition. Front. Microbiol. 2016;7:1575. doi:
  14. Li F, Guan LL. Metatranscriptomic profiling reveals linkages between the active rumen microbiome and feed efficiency in beef cattle. Appl Environ Microbiol. 2017;83(9): e00061-17. doi: 10.1128/AEM.00061-17
  15. Lima J, Auffret MD, Stewart RD, Dewhurst RJ, Duthie CA, Snelling TJ, Walker AW, Freeman TC, Watson M, Roehe R. Identification of rumen microbial genes involved in pathways linked to appetite, growth, and feed conversion efficiency in cattle. Front Genet. 2019;10:701. doi:
  16. Mihaylova D, Vrancheva R, Petkova N, Ognyanov M, Desseva I, Ivanov I, Popova M, Popova A. Carotenoids, tocopherols, organic acids, charbohydrate and mineral content in different medicinal plant extracts. Zeitschrift für Naturforschung C A Journal of Biosciences. 2018;73(11-12):439-448. doi:
  17. Muhammed AA, He J. Use of probiotics and botanical extracts to improve ruminant production in the tropics: а review. Animal Nutrition. 2018;4(3):241-249. doi:
  18. Myer PR, Smith TPL, Wells JE, Kuehn LA, Freetly HC. Rumen microbiome from steers differing in feed efficiency. PLoS One. 2015; 10(6):e0129174. doi:
  19. Paz HA, Anderson CL, Muller MJ, Kononoff PJ, Fernando SC. Rumen bacterial community composition in Holstein and Jersey cows is different under same dietary condition and is not affected by sampling method. Front Microbiol. 2016;7:1206. doi:
  20. Peng M, Wang Z, Peng S, Zhang M, Duan Y, Li F, Shi S, Yang Q, Zhang C. Dietary supplementation with the extract from Eucommia ulmoides leaves changed epithelial restitution and gut microbial community and composition of weanling piglets. PLoS One. 2019;14(9):e0223002. doi:
  21. Schären M, Frahm J, Kersten S, Meyer U, Hummel J, Breves G, Dänicke S. Interrelations between the rumen microbiota and production, behavioral, rumen fermentation, metabolic, and immunological attributes of dairy cows. J Dairy Sci. 2018;101(5):4615-4637. doi:
  22. Shabat SKB, Sasson G, Doron-Faigenboim A, Durman T, Yaacoby S, Berg Miller ME, White BA, Shterzer N, Mizrahi I. Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants. ISME J. 2016;10(12):2958-2972. doi:
  23. Tolmacheva AA, Rogozhin EA, Deryabin DG. Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants. Acta Pharmaceutica. 2014;64(2):173-186. doi:
  24. Tong J, Zhang H, Yang D, Zhang Y, Xiong B, Jiang L. Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows. PLoS ONE. 2018;13(11):e0198225. doi:
  25. Valenzuela-Grijalva NV, Pinelli-Saavedra A, Muhlia-Almazan A, Domínguez-Díaz D, González-Ríos H. Dietary inclusion effects of phytochemicals as growth promoters in animal production. Journalofanimalscienceandtechnology. 2017;59:8. doi: 10.1186/s40781-017-0133-9

Atlanderova Kseniya Anatolevna, Junior Researcher, Test Centre CUC, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., e-mail:

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Deputy Director for Science, Head of the Farm Animal Feeding and Feed Technology Department named after SG Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail:

Makaeva Aina Maratovna, Junior Researcher of the Centre "Nanotechnologies in Agriculture", Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-919-842-46-99, e-mail:

Muslyumova Dina Marselyevna, Cand. Sci (Biol.), Senior Research Assistant of Test Centre CUC, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.:8(3532)30-81-72, e-mail:

Kondrashova Kristina Sergeevna, Junior Researcher at the Laboratory For Breeding And Genetic Research, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.

Received: 29 November 2019; Accepted: 16 December 2019;Published: 31 December 2019