Klimova TA, Sizentsov AN, Sizentsov YaA, Gubaidullina IZ

DOI: 10.33284/2658-3135-102-4-208

UDC 599.323.4:577.17


The studies were supported by the Regional Grant No. 21 dated 14.08.2019.

The effect of probiotic strains of Bacillus genus on blood biochemical parameters

and copper concentration in animals

Tatijana A Klimova1,2, Aleksey N Sizentsov2, Yaroslav A Sizentsov2, Ilmira Z Gubaidullina1

1 Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

2Orenburg State University (Orenburg, Russia)

Summary. The article presents the results of experimental studies on the effective use of Bacillus probiotic strains of animals with copper deficiency (for example, rats). Bacillus bacteria: Vetom 2 (B. subtilis 7048 strain and B. licheniformis 7038 strain) and Sporobacterin (B. subtilis 534 strain) were used as a probiotic agent. CuSO4 × 5H2O, which creates high element concentrations in nutrient substrate, acted as a regulatory factor for the excess concentration of copper ions in the experiment. The effectiveness of use of the obtained biologically active compounds was evaluated using atomic absorption spectrophotometry; skin, muscle and bone tissue were used as biological samples. The obtained data indicate a high potential for the use of probiotic drugs, which can stimulate the accumulation of copper in the body. So, in the samples of the experimental groups, the concentration of the test element exceeded the control group with a low copper content in the diet by 98.61%, 108.33%, and 81.94% at the end of the study. Based on the assessment of the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase in blood serum, there was no pathological effect of the tested compounds on hepatocytes, as evidenced by the dynamics of the studied parameters within the reference values ​​throughout the experiment.

Key words: animals, rats, feeding, copper, probiotic preparations, bacteria of Bacillus genus, mineral deficiency states.


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Klimova Tatiana AndreevnaБspecialist-technicianof Test Centre CUC, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg,Russia, 29, 9 Yanvarya St.; postgraduate student, 460018,Orenburg, Russia, Orenburg State University, 13 Pobedy Ave.; tel.: +79878494166, e-mail: klimovat91@mail.ru

Sizentsov Aleksey Nikolaevich, Cand. Sci. (Biol.), Associate Professor of the Department of Biochemistry and Microbiology, 460018,Orenburg, Russia, Orenburg State University, 13 Pobedy Ave., tel.: +79058803604, e-mail: asizen@mail.ru

Sizentsov Yaroslav Alekseevich, student, Orenburg State University, 460018,Orenburg, Russia, Orenburg State University, 13 Pobedy Ave., tel.: +79058803604, e-mail: asizen@mail.ru

Gubaidullina Ilmira Zakievna, postgraduate student of veterinary medicine and zootechnology, junior research associate of Laboratory for Biological Testing and Expertises,Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, е-mail: gubaidullinae@mail.ru, tel.: 8-912-843-10-69

Received: 13 December 2019; Accepted: 16 December 2019;Published: 31 December 2019