Lebedev SV, Sheyda EV, Vershinina IA, Gubaidullina IZ, Miroshnikov IS, Ryazanov VA, Makaeva AM, Markova IV, Ushakov AS

DOI: 10.33284/2658-3135-102-4-198

UDC 665.32/.35:636.085.25

Acknowledgements:

The work is funded by the Ministry of Science and Education of the Russian Federation to carry out fundamental scientific research determined by the Presidium of the Russian Academy of Sciences (Agreement No. 075-02-2019-1847).

The influence of various sources of fat in the diet on digestibility and activity of digestive enzymes

of calves 

Svyatoslav V Lebedev1,2, Elena V Sheyda2, Irina A Vershinina1, Ilmira Z Gubaidullina1,

Ivan S Miroshnikov1, Vitaly A Ryazanov1,Aina M Makaeva1, Irina V Markova1, AlexanderS Ushakov3 

1Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia) 2Orenburg State University (Orenburg, Russia) 3All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition– branch of the Federal Science Center for Animal Husbandry named after Academy Member LK Ernst (Borovsk, Russia) Summary. Up to now the question of the ability of pancreas to secrete pancreatic juice, corresponding to the various ingredients of the diet in terms of quantity and enzymatic composition, has not been fully studied. There is evidence in the literature that indicates the ability of pancreas to change the composition of its secretion according to the nature of food consumed. In world practice, such studies, due to the methodological difficulty in obtaining pure pancreatic juice, are focused on studies of the qualitative composition of chyme and do not allow studying the reflex and neurohumoral mechanisms of adaptation of pancreatic secretion to the quality of food eaten. As a result of our studies using a surgical operation to form an isolated segment of the intestine with a flowing pancreatic duct, we gained new knowledge about the exocrine function of pancreas, the absorption of nutrients by cattle after different feeds were used in the diet. The study of exocrine pancreatic function was carried out on 3 calves of the Kazakh white-headed breed at the age of 8 months with an average weight of 115-120 kg. The introduction of palm oil in the diet had a stimulating effect on digestibility of organic matter by 12.1%, crude protein - by 8.5% amid a decrease in the digestibility of fat by 27.6-45.8% and crude fiber - to 34-35 % The role of digestive enzymes in the regulation of the digestion process was to reduce pancreatic juice exosecretion in experimental groups by 19% and 6%, respectively. The inclusion of sunflower oil in the diet stimulated the synthesis of lipase and protease by 79.3% and 38.3% and depression of amylase production by 44.3%. A similar dynamics is typical for palm fat. Regardless of the fatty acid composition of the fat, the protease-amylase and lipase-amylase ratios decreased and the lipase-protease ratio increased. Thus, according to the studied parameters, the most digestible form of vegetable fats for cattle is sunflower oil, since its participation in metabolic processes occurs without tension in the metabolism. Key words: cattle, feeding, digestibility of nutrients, fat, palm oil, sunflower oil, pancreas.   References
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Lebedev Svyatoslav Valerevich, Dr. Sci. (Biol.), Deputy Director, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-912-345-87-38,e-mail: lsv74@list.ru; Associate Professor, Department of Biotechnology of Animal Raw Materials and Aquaculture, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave.

Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Experimental Biological Clinic, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave. tel.: 8-987-862-64-05, e-mail: elena-shejjda@mail.ru

Vershinina Irina Aleksandrovna, junior research associate of Laboratory for Biological Testing and Expertises,Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  460000, е-mail:gavrish.irina.ogu@gmail.com, tel.: 8-987-798-67-88

Gubaidullina Ilmira Zakievna, postgraduate student of veterinary medicine and zootechnology, Junior Researcher associate of Laboratory for Biological Testing and Expertises,Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., е-mail: gubaidullinae@mail.ru, tel.: 8-912-843-10-69

Miroshnikov Ivan Sergeevich, Cand. Sci. (Agr.), ResearcherofFarm Animal Feeding and Feed Technology Department named after SG. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79, е-mail: vniims.or@mail.ru

Ryazanov Vitaly Aleksandrovich, Cand. Sci. (Agr.), ResearcherofFarm Animal Feeding and Feed Technology Department named after SG. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79, e-mail: vniims.or@mail.ru, vita7456@yandex.ru

Makaeva Aina Maratovna, Junior Researcher of the Centre "Nanotechnologies in Agriculture", Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-919-842-46-99, e-mail: ayna.makaeva@mail.ru

Markova Irina Viktorovna, Cand. Sci. (Biol.), Head of the Research and Educational Centre,Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., е-mail: nocbst@mail.ru; tel.: 8-961-047-40-26

Ushakov Alexander Sergeyevich, Cand. Sci (Biol.), Senior Researcher, Laboratories Of Physiology Of Digestion And Interstitial MetabolismAll-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition– branch of the Federal Science Center for Animal Husbandry named after Academy Member LK Ernst 249013, Kaluga Region, Borovsk, Russia, e-mail: asu2004@bk.ru

Received: 29 November 2019; Accepted: 16 December 2019;Published: 31 December 2019

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