Elena V Sheyda, Svyatoslav V Lebedev, Sergey A Miroshnikov, Galimzhan K Duskaev, Vitaly A Ryazanov, Victoria V Grechkina, Shamil G Rakhmatullin

DOI: 10.33284/2658-3135-104-2-84

UDC 665.345.4:636.085.25

Acknowledgements:

The research was carried out with the support of the Russian Science Foundation. FSBSI FRC BST RAS (No. 20-16-00088)

Effect of supplemental flaxseed oil on altering of rumen microbiome of cattle

Elena V Sheyda1,2, Svyatoslav V Lebedev2, Sergey A Miroshnikov2,1, Galimzhan K Duskaev2,

Vitaly A Ryazanov2, Victoria V Grechkina2,3, Shamil G Rakhmatullin2

1Orenburg State University (Orenburg, Russia)

2Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

3Orenburg State Agrarian University (Orenburg, Russia)

Summary. High-quality feeding is the key to obtaining high-class agricultural products. In order to optimize feeding and correct the nutrient composition of diets, numerous studies aimed at studying the taxonomic composition of the rumen are done. The quantitative and qualitative compositions of rumen microbiome cannot directly increase feed digestibility and yield of finished products, but they will provide knowledge that will help to develop new rational dietary interventions to improve feeding efficiency and increase productivity. The aim of the study was to study the degree of digestibility of the dry matter (DM) of the diet and the effect on the composition of the rumen microflora by additional administration of flaxseed oil (3 % of the DM of the diet) in vitro using the "artificial rumen" model on the "ANKOM Daisy II" incubator (modifications D200 and D200I). The study of rumen microbiome was carried out on a model of bulls with a rumen fistula (n=3). Microflora analysis was performed using MiSeq ("Illumina", USA) by the new generation sequencing (NGS) method. With the introduction of flaxseed oil, an increase in the digestibility of SV feed by 2.3% was noted. The additional inclusion of flaxseed oil in the diet of cattle contributed to an increase in the species diversity of the rumen microbiota, the number of bacteria of Bacteroidetes (64.2%), as well as a significant increase in representatives of the archaea domain involved in rumen methanogenesis. It was found that the level of species diversity was 45% higher with the introduction of flaxseed oil than in the control. The Simpson index in the control group was 0.44, in the experimental group – 0.54. The most pronounced change in the level of diversity was observed in the experimental group, the Shannon index (P≤0.05) was 0.76. The indices of β-diversity of Jaccard and Sørensen indicate a complete coincidence of the communities in the control and experimental groups.

Keywords: cattle, feeding, flaxseed oil, microbiome, rumen, digestibility.

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Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.; Senior Researcher, Experimental Biological Clinic, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave, 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

Lebedev Svyatoslav Valerevich, Dr. Sci. (Biol.), Leading Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-912-345-87-38, e-mail: lsv74@list.ru

Miroshnikov Sergey Aleksandrovich, Dr. Sci (Biol.), RAS Corresponding Member, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-70, e-mail: fncbst@mail.ru; Interim Rector, Orenburg State University, 460018, Orenburg, Russia, 13 Pobedy Ave, tel.:77-67-70, e-mail: rector_osu@mail.osu.ru

Grechkina Victoria Vladimirovna, Cand. Sci (Biol.), Acting Head of Laboratory for Biological Testing and Expertise, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., Associate Professor, Department of Noncommunicable Animal Diseases, Orenburg State Agrarian University, 460014, Orenburg, Russia, 18 Chelyuskintsev, St.,  tel.: 8-922-877-14-97, e-mail: Viktoria1985too@mail.ru

Ryazanov Vitaly Aleksandrovich, Cand. Sci. (Agr.), Researcher of Farm Animal Feeding and Feed Technology Department named after Leushin SG., Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79, e-mail: vita7456@yandex.ru

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Leading Researcher, Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail: gduskaev@mail.ru

Rakhmatullin Shamil Gafiullovich, Cand. Sci. (Biol.), Senior Researcher of the Department of Feeding Farm Animals and Feed Technology named after S.G. Leushin, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., tel.: 89228157225, е-mail: shahm2005@rambler.ru

Received: 27 May 2021; Accepted: 15 June 2021; Published: 30 June 2021

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