Baer S Nurzhanov, Galimzhan K Duskaev, Olga V Kwan, Elemes A Azhmuldinov

DOI: 10.33284/2658-3135-104-3-167

UDC 636.085.25:633.88

Acknowledgements:

Research was carried out according the plan of research scientific works on 2021-2023 yy. FSBSI FRC BST RAS (No 0761-2019-0005)

Changes in metagenomic composition of rumen under the influence of herbal extracts

 Baer S Nurzhanov, Galimzhan K Duskaev, Olga V Kwan, Elemes A Azhmuldinov

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

 Abstract. Legislative restrictions on the use of feed antibiotics have led to the search for new feed products of natural origin with similar properties. Phytobiotics stand out from a wide variety of feed additives. They have immuno- and growth-stimulating, as well as antioxidant properties. According to the study scheme, animals in the control group received BD, experimental I - BD + Oak bark extracts (OBE) 33.3% + birch leaves (BL) 33.3% + St. John's wort (ETZ) 33.3%, experimental II – BD + Oak bark extracts 50% + birch leaves 50%, III experimental - BD + Extracts of oak bark 50% + St. John's wort 50%. The extracts were administered with individual drinkers at the rate of 1.66 ml/kg body weight. Genomic DNA was isolated from control and experimental samples using the chemical extraction method. According to the results of in situ studies, it was found that the digestibility of the dry matter of the grain mixture with the introduction of extracts of oak bark 33.3% + birch leaves 33.3% + St. John's wort herb 33.3% was higher compared to groups II and III by 11.98 and 13.52%. The group that received 50% oak bark extract and 50% St. John's wort extract was found to have the least digestibility of dry matter of the grain mixture; this indicator was 1.54% lower in comparison with group II. The use of oak bark extract 33.3% + birch leaves 33.3% + St. John's wort 33.3% contributed to an increase in the number of bacteria belonging to the phylum Firmicutes by 5.65 and 6.24% from groups II and III. The introduction of extracts of oak bark and birch leaves promoted better development of bacteria of the families Muribaculaceae (by 2.49 and 1.14%), Prevotellaceae (by 9.06 and 8.07%), and Candidatus Saccharibacteria (by 0.06 and 0.34%) in comparison with groups I and III.

Keywords: bulls, rumen, feeding, digestibility, extracts, metagenome, bacteria.

 References

  1. Koloskova EM, Ezerskiy VA, Ostrenko KS, Ovcharova AN, Belova NV. Studies of the sheep rumen microbiome using molecular genetic methods: a review. Problems of the Biology of Productive Animals. 2020;4:5-26. doi: 10.25687/1996-6733.prodanimbiol.2020.4.5-26
  2. Abreu AC, McBain AJ, Simoes M. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep. 2012;29(9):1007-1021. doi: 10.1039/c2np20035j
  3. Duskaev GK, Kazachkova NM, Ushakov AS, Nurzhanov BS, Rysaev AF. The effect of purified Quercus cortex extract on biochemical parameters of organism and productivity of healthy broiler chickens. Vet World. 2018;11(2):235-239. doi: 10.14202/vetworld.2018.235-239
  4. Harlow BE, Flythe MD, Kagan IA, Goodman JP, Klotz JL, Aiken GE. Isoflavone supplementation, via red clover hay, alters the rumen microbial community and promotes weight gain of steers grazing mixed grass pastures. PLoS One. 2020;15(3):e0229200. doi: 10.1371/journal.pone.0229200
  5. Huyghebaert G, Ducatelle R, Van Immerseel F. An update on alternatives to antimicrobial growth promoters for broilers. Veterinary Journal. 2011;187(2):182-188. doi: 10.1016/j.tvjl.2010.03.003
  6. 6. Karimov I, Kondrashova K, Duskaev G,  Kvan O. Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli. In: Zhou P, He Y, Weerasinghe R, editors. E3S Web of Conferences: 2nd International Symposium on Architecture Research Frontiers and Ecological Environment (ARFEE 2019); 2019 December 20-22; Guilin, China. Les Ulis, France: EDP Science; 2020; 143:02034. doi: https://doi.org/10.1051/e3sconf/202014302034
  7. Kasparovska J, Pecinkova M, Dadakova K, Krizova L, Hadrova S, Lexa M, Lochman J, Kasparovsky T. Effects of isoflavone-enriched feed on the rumen microbiota in dairy cows. PLoS One. 2016;:11(4):e0154642. doi: 10.1371/journal.pone.0154642
  8. Li D, Zhang Y, Cui Z, He L, Chen W, Meng Q, Ren L. Effects of phytoecdysteroids (PEDS) extracted from cyanotis arachnoidea on rumen fermentation, enzyme activity and microbial efficiency in a continuous-culture system. PLoS One. 2016;11(4): e0153584. doi: 10.1371/journal.pone.0153584
  9. Logachev K, Karimov I, Duskaev G, Frolov A, Tulebaev S, Zav'yalov O. Study of intercellular interaction of ruminal microorganisms of beef cattle. Asian Journal of Animal Sciences. 2015;9(5):248-253.
  10. Muhammed A Arowolo, Jianhua He. Use of probiotics and botanical extracts to improve ruminant production in the tropics: A review. Anim Nutr. 2018;4(3):241-249. doi: https://doi.org/10.1016/j.aninu.2018.04.010
  11. Newman DJ, Gordon MC. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629-661. doi: 10.1021/acs.jnatprod.5b01055
  12. Oh S, Koike S, Kobayashi Y. Effect of ginkgo extract supplementation on in vitro rumen fermentation and bacterial profiles under different dietary conditions. Anim Sci. J. 2017;88(11):1737-1743. doi: 10.1111/asj.12877
  13. Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complement Altern Med. 2011;8(1):1-10.
  14. Tolmacheva AA, Rogozhin EA, Deryabin DG. Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants. Acta Pharmaceutica. 2014;64(2):173-186. doi: 10.2478/acph-2014-0019
  15. Valenzuela-Grijalva NV, Pinelli-Saavedra A, Muhlia-Almazan A, Domínguez-Díaz D, González-Ríos H. Dietary inclusion effects of phytochemicals as growth promoters in animal production. J Anim Sci Technol. 2017;59:8. doi: 10.1186/s40781-017-0133-9
  16. Xu Y, Tao Z, Jin Y, Yuan Y, Dong TTX, Tsim KWK, Zhou Z. Flavonoids, a potential new insight of leucaena leucocephala foliage in ruminant health. J Agric Food Chem. 2018;66(29):7616-7626 doi: 10.1021/acs.jafc.8b02739

Nurzhanov Baer Serekpaevich, Cand. Sci (Agr.), Senior Researcher of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., e-mail: baer.nurzhanov@mail.ru

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Leading Researcher, Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail: gduskaev@mail.ru

Kwan Olga Vilorievna, Cand. Sci. (Biol.), Acting Head of the Department of Feeding Farm Animals and Feed Technology named after S.G. Leushin, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., tel.: 8-922-548-56-57,e-mail: kwan111@yandex.ru

Azhmuldinov Elemes Azhmuldinovich, Dr. Sci (Agr.), Professor, Chief Researcher, Department of Technology of Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78

Received: 6 August 2021; Accepted: 13 September 2021; Published: 30 September 2021

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