Shamil G Rakhmatullin, Bayer S Nurzhanov, Galimzhan K Duskaev, Olga V Kwan, Elena V Sheyda

DOI: 10.33284/2658-3135-104-3-94

UDC 636.084.1

Acknowledgements:

Research was carried out according the plan of research scientific works on 2021-2023 yy. FSBSI FRC BST RAS (No 0761-2019-0005)

Influence of plant extracts on rumen metagenome

 Shamil G Rakhmatullin, Bayer S Nurzhanov, Galimzhan K Duskaev, Olga V Kwan, Elena V Sheyda

Federal Research Centre of Biological System and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Abstract. In this work, a change in the taxonomic composition of the microbiome of the gastrointestinal tract of cattle was revealed when plant extracts (extracts) were included in the animal diet. According to the study scheme, the animals of the control group received basic diet (BD), I experimental – BD + extracts of birch leaves (EBL) 50% + St. John's wort grass (JWG) 50%, II experimental – BD + extracts of oak bark 50% + St. John's wort grass 50%. The extracts were administered to individual drinkers at the rate of 1.66 ml/kg of body weight. The introduction of EBL 50% + JWG 50 % to animals of group I allowed to increase the number of bacteria of the Bacteroidia class by 1.99% and to reduce the number of bacteria by 0.74% from the Clostridia class and the Negativicutes class by 1.39% compared to the control group. The largest share of species diversity was represented by Prevotella – 11.96% of the total number, indeterminate Bacteroid-25.08% of the total number, indeterminate Lachnospiraceae – 4.73% of the total number, indeterminate Ruminococcaceae – 10.56% of the total number, Succiniclastricum – 6.92 % of the total number. When introducing extracts of oak bark 50% + St. John's wort herb 50 % to bulls, a decrease in the number of bacteria of the Lachnospiraceae family (by 2.16 and 2.71%) and Ruminococcaceae (by 2.52 and 3.1%) was observed relative to the control and I group.

Keywords: bulls, Red Steppe Breed, feeding, rumen, extract, taxonomic composition.

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Rakhmatullin Shamil Gafiullovich, Cand. Sci. (Biol.), Senior Researcher of the Department of Feeding Farm Animals and Feed Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., tel.: 8-922-815-72-25, е-mail: shahm2005@rambler.ru

Nurzhanov Baer Serekpaevich, Cand. Sci (Agr.), Senior Researcher of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., e-mail: baer.nurzhanov@mail.ru

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Leading Researcher, Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail: gduskaev@mail.ru

Kwan Olga Vilorievna, Cand. Sci. (Biol.), Acting Head of the Department of Feeding Farm Animals and Feed Technology named after S.G. Leushin, Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., tel.: 8-922-548-56-57,e-mail: kwan111@yandex.ru

Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

 Received: 9 September 2021; Accepted: 13 September 2021; Published: 30 September 2021

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