Victoria V Grechkina, Elena V Sheyda, Svyatoslav V Lebedev, Irina V Markova, Nikolay I Ryabov

 DOI: 10.33284/2658-3135-104-3-47

UDC 636.084.1:636.085.25

Acknowledgements:

Research was carried out according the plan of research scientific works on 2021-2023 yy. FSBSI FRC BST RAS (No 0761-2019-0005)

Nutrient digestibility and nitrogen balance in calves depending on the level of amino acid nutrition

 Victoria V Grechkina, Elena V Sheyda, Svyatoslav V Lebedev, Irina V Markova, Nikolay I Ryabov

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

 Abstract. Protein is considered a key nutrient in animal feeding, not only providing amino acids, but also being a source of nitrogen (N) for the synthesis of microbial protein. The requirements for amino acids are based on the need for pure protein and the profile of amino acids in the tissues of the whole body. The work studied the digestibility of nutrients and the nitrogen balance in calves of the Kazakh white-headed breed when introducing a mixture of essential amino acids into the diet. The objects of the study are calves of the Kazakh white-headed breed aged from 9 to 18 months of age. The source of additional amino acids were: methionine, lysine, histidine, threonine, tryptophan. The level of essential amino acids in some body tissues was in the I experimental group (7.87%) and the II experimental group (10.85%) (P≤0.05) relative to the control values. The level of digested nitrogen in the duodenum increased in I – 17.82% and II – 21.34%, (P≤0.05). Thus, the animals receiving the concentration of essential amino acids at a dose of 3 g of lysine + 3 g of methionine + 4 g of threonine + 2 g of tryptophan were distinguished by better digestibility, protein synthesis and productive qualities.

Keywords: calves, Kazakh white-headed breed, diet, amino acids, digestibility of substances, metabolism, proteins.

References

  1. Golovko EH. Availability of amino acids in the nutrition of monogastric animals. (Conference proceedings) Amino acid nutrition of animals and the problem of protein resources: conf. materials (Krasnodar, March 23, 2004) ed. and with a foreword. Ryadchikova VG. Krasnodar: Publishing House KubSAU; 2005:71-118.
  2. GOST 13496.4-93 Stern, compound feeds, formula-feed raw materials. Methods for determining nitrogen and crude protein content. Intro. 01.01.1995. Moscow: Standardinform; 1993:17 р.
  3. GOST 26226-95. GOST 26226-95. Stern, compound feeds, formula-feed raw materials. Crude ash determination methods. Intro. 01.01.1977. Moscow: PPC Publishing house of standards; 1996:8 p.
  4. GOST 31675-2012. Methods for determination of raw fiber content using intermediate filtration. Intro. 01.07.2013. Moscow: Standardinform; 2012:18 p.
  5. GOST 13496.15-97. Stern, compound feeds, formula-feed raw materials. Methods for determining the content of raw fat (with Revision No. 1). 01.01.1999. Moscow: Standartinform. 1997:19 р.
  6. Kalashnikov AP et al. Standards and diets of farm animals: Ref. book. 3rd ed., rework. and add. Moscow: Agropromizdat; 2003:456 p.
  7. Lebedev SV, Gubaidullina IZ, Sheida EV, Grechkina VV. Exchange (uptake and synthesis) of amino acids in the digestive tract of cattle when used in diet different ingredient composition of the feed. The Agrarian Scientific Journal. 2019;4:54-57. doi: https://doi.org/10.28983/asj.y2019i4pp54-57.
  8. Akimov SS, Lebedev SV, Grechkina VV, Miroshnikova MS, Topuria GM. The effectiveness of using mathematical modeling in assessing the quality of food products. IOP Conf Series: Earth and Environmental Science: International Conference on World Technological Trends in Agribusiness; 2020 4-5 July, Omsk City, Western Siberia, Russian Federation. Bristol, England: IOP Publishing; 2021;624:012158. doi: 10.1088/1755-1315/624/1/012158
  9. Dhama K, Latheef SK, Mani S, Samad HA et al. Multiple beneficial applications and modes of action of herbs in poultry health and production – a review. J Pharmacol. 2015;11(3):152-176. doi: 10.3923/ijp.2015.152.176
  10. Goyal AK, Brahma BK. Antioxidant and nutraceutical potential of bamboo: an overview. J Fundamental Appl Sci. 2014;3(1):2-10.
  11. Grechkina VV, Miroshnikov SA, Lebedev SV. Lipid spectrum of blood when vegetable fats are introduced into the diet of calves. IOP Conf Series: Earth and Environmental Science: International Conference on World Technological Trends in Agribusiness; 2020 4-5 July, Omsk City, Western Siberia, Russian Federation. Bristol, England: IOP Publishing;2021;624:012025. doi: 10.1088/1755-1315/624/1/012025
  12. Kang JX, Wan JBo, He C. Concise review: regulation of stem cell proliferation and differentiation by essential fatty acids and their metabolites. Stem Cells. 2014;32(5):1092-1098. doi: https://doi.org/10.1002/stem.1620
  13. Kimball SR, Jefferson LS. Signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis. J Nutr. 2006;136(1):227S-231S. doi: https://doi.org/10.1093/jn/136.1.227S
  14. Li S, Li X. Leptin in normal physiology and leptin resistance. Sci Bull. 2016;61(19):1480-1488. doi: https://doi.org/10.1007/s11434-015-0951-4
  15. Liu Z, Long W, Frybarg DA, Barret EJ. The regulation of body and skeletal muscle protein metabolism by hormones and amino acids. J Nutr. 2006;136(1):212S-217S. doi: https://doi.org/10.1093/jn/136.1.212S
  16. Malesci A, Gaia E, Fioretta A et al. No effect of long-term treatment with pancreatic extract on recurrent abdominal pain in patients with chronic pancreatitis. Scand J Gastroenterol. 1995;30(4):392-398. doi: https://doi.org/10.3109/00365529509093296
  17. Nakamura М, Nomura S, Yamakawa T et al. Endogenous calcitonin regulates lipid and glucose metabolism in diet-induced obesity mice. Scientific Reports. 2018;8:17001. doi: 10.1038/s41598-018-35369-5
  18. 18. Pang J, Xi C, Huang X, Cui J, Gong H, Zhang T. Effects of excess energy intake on glucose and lipid metabolism in C57BL/6 Mice. PLoS ONE. 2016;11(1):e0146675. doi: https://doi.org/10.1371/journal.pone.0146675
  19. Tang Q. Lipid metabolism and diseases. Sci. Bull. 2016;61(19):1471-1472. doi: 10.1007/s11434-016-1174-z
  20. Xie T et al. An ErChen and YinChen decoction ameliorates high-fat-induced nonalcoholic steatohepatitis in rats by regulating JNK1 signaling pathway. Evidence-Based Complementary and Alternative Medicine. 2017;4603701. doi: https://doi.org/10.1155/2017/4603701
  21. Yang HJ, Yim N, Lee KJ et al. Simultaneous determination of nine bioactive compounds in Yijin-tang via high-performance liquid chromatography and liquid chromatography-electrospray ionization-mass spectrometry. Integrative Medicine Research. 2016;5(2):140-150. doi: https://doi.org/10.1016/j.imr.2016.04.005
  22. Zdunczyk Z, Jankowski J, Kaczmarek S, Juskiewicz J. Determinants and effects of postileal fermentation in broilers and turkeys. Part 1: gut microbiota composition and its modulation by feed additives. World’s Poult Sci J. 2015;71(1):37-48. doi: 10.1017/S0043933915000045
  23. Zhou J, Liu H, Zhou S et al. Adaptor protein APPL1 interacts with EGFR to orchestrate EGF-stimulated signaling. Sci Bull. 2016;61(19):1504-1512. doi: https://doi.org/10.1007/s11434-016-1157-0

Grechkina Victoria Vladimirovna, Cand. Sci (Biol.), Acting Head of Laboratory for Biological Testing and Expertise, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8-922-877-14-97, e-mail: Viktoria1985too@mail.ru

Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

Lebedev Svyatoslav Valerevich, Dr. Sci. (Biol.), Leading Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-912-345-87-38, e-mail: lsv74@list.ru

Markova Irina Viktorovna, Cand. Sci. (Biol.), Researcher Department of Technology of Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., 29, tel.: 8-961-047-40-26, e-mail: irinazzz88@yandex.ru

Ryabov Nikolai Ivanovich, Dr. Sci. (Agr.), Leading Researcher, Department for Beef Cattle Technology and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St.

Received: 2 July 2021; Accepted: 13 September 2021; Published: 30 September 2021

Download