Evgenia S Surzhikova, Мarina P Dubovskova, Nikolay P Gerasimov

Animal Husbandry and Fodder Production. 2021. Vol. 104, no 4. Р. 67-78.

 doi:10.33284/2658-3135-104-4-67

  Polymorphism of genes CAPN1(c. 316 C>G), TG5(c.-422C>T), GH(c.2141C>G), LEP(c.73C>T) in young beef animals of Stavropol population Hereford breed

 Evgenia S Surzhikova1, Мarina P Dubovskova2, Nikolay P Gerasimov3

1North Caucasus Federal Agrarian Research Center, Mikhailovsk, Russia

2,3Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1lab.dna @fnac.center, https //orcid org/ 0000-0002-3955-0902

2dubovskova.m@mail.ru, https://orcid.org/0000-0001-6915-4647

3nick.gerasimov@rambler.ru, https://orcid.org/0000-0003-2295-5150

Abstract. Recently, due to the use of advances in molecular genetic research, significant successes have been achieved in meat cattle breeding. The issue of introducing progressive DNA technologies is not accidentally in the spotlight of specialists working in market cattle breeding, since attracting information about genetic markers changes the value of animals. The purpose of these studies was to evaluate the genetic profile of repair young growth of Hereford cattle breed on the basis of DNA-software testing to polymorphisms genes CAPN1 (with. 316 C > G), TG5 (c.-422C > T), GH (c.2141C > G), LEP (c.73C > T), which were bred in the breeding farm of the Stavropol Territory. PCR-PDRF methods genotyped repair young Hereford (n = 103).

As a result of the studies, the polymorphism of the studied genes was determined. Polymorphism is represented by two alleles: CAPN1C and CAPN1G, TGТ and TGC, GHG and GH CLEPТиLEPС, three genotypes: CAPN1СС, CAPN1GG, CAPN1СG; TGТТ, TGCC, TGТC; GHGG, GHCC, GHCG;LEPСС, LEPТТ, LEPСТ with significant frequency variability of both alleles and genotypes, which was: for alleles - from minimum 0.15, to maximum values ​ ​ 0.85, genotypes - from minimum values ​2.0% to maximum 75%. It was found that the presence of the desired gene complex of four alleles which consist from four to three genes (TGTС/GHGG/LEPТT or CAPN1CG/TGTС/GHCG/LEPCT)  in calves of the Hereford breed was 21.4%, in heifers - 10.0%. Most of the herd (64.3-46.7%) were carriers of two marker alleles which consist from two or one gene. For the first time, the specificity of the allelic profile of genes CAPN1(c. 316 C>G), TG5 (c.-422C>T), GH(c.2141C>G), LEP(c.73C>T) of repair young Hereford breed, especially valuable genotypes were identified, their specific gravity was determined for wide use in the practical selection of the Stavropol Territory.

Keywords: cattle, herd replacements, Hereford breed, polymorphism, gene complex, genotype, CAPN1, TG, GH, LEP, Stavropol Territory

Acknowledgments: the work was performed in accordance to the plan of research works for 2021-2023 FSBRI FRC BST RAS (No. 0526-2021-0001).

For citation: Surzhikova ES, Dubovskova МP, Gerasimov NP. Polymorphism of genes CAPN1(c. 316 C>G), TG5(c.-422C>T), GH(c.2141C>G), LEP(c.73C>T) in young beef animals of Stavropol population Нereford breed. Animal Husbandry and Fodder Production. 2021;104(4):67-78. (In Russ.). https://doi.org/10.33284/2658-3135-104-4-67

References

  1. Zinovieva NA, Sermyagin AA, Dotsev AV, Boronetslaya OI, Petrikeeva LV, Abdelmanova AS, Brem G. Animal genetic resources: developing the research of allele pool of Russian cattle breeds – minireview. Agricultural Biology. 2019;54(4):631-641. doi: 10.15389/agrobiology.2019.4.631eng
  2. Novikov AA, Semak MS, Kalashnikova LA. The need to improve the system of genetic expertise of breeding products in the Russian Federation. Zootechniya. 2021;6:2-6. doi: 10.25708/ZT.2021.17.85.001
  3. Selionova MI, Chizhova LN, Bobryshova GT, Surzhikova ES, Mikhaylenko AK. Perspective genetic markers of horned cattle. Agricultural Bulletin of Stavropol Region. 2018;3(31):44-51. doi: 10.31279/2222-9345-2018-7-31-44-51
  4. Kovaljuk NV, Satsuk VF, Volchenko AE, Machulskaja EV. LEP gene allelic polymorphism in a subpopulation of ayrshire cattle. Russian Journal of Genetics. 2015:51(2):214-217. doi: 10.1134/S1022795415020106
  5. Gorlov IF, Fedunin AA, Randelin DA, Sulimova GE. Polymorphisms of bGH, RORC, and DGAT1 genes in Russian beef cattle breeds. Russian Journal of Genetics. 2014;50(12):1302-1307. doi: 10.1134/S1022795414120035
  6. Tjulkin SV, Ahmetov TM, Valiullina EF, Vafin RR. Polymorphism of genes for somatotropin, prolactin, leptin, and thyroglobulin in stud bulls. Vavilov Journal of Genetics and Breeding. 2012;16(4-2):1008-1012.
  7. Yulmeteva YuR, Shakirov ShK. The association of the thyroglobulin gene with the productive longevity of dairy cattle. Dairy and Beef Cattle Farming. 2020;1:14-19. doi: 10.33943/MMS.2020.65.47.004
  8. Barendse W, Bunch R, Thomas M, Armitage S, et al. The TG5  thyroglobulin  gene  test  for  a  marbling  quantitative  trait  loci  evaluated  in  feedlot  cattle.  Aust J Exp Agr. 2004;44(7):669-674. doi: 10.1071/EA02156
  9. Buchanan FC, Fitzsimmons CJ, Van Kessel AG, Thue TD, Winkelman-Sim DC, Schmutz SM. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet Sel Evol. 2002; 34(1):105-116. doi: 10.1051/gse:2001006
  10. Coates BS, Sumerford DV, Miller NJ, Kim KS, Sappington TWet al. Comparative performance of single nucleotide polymorphism and microsatellite markers for population genetic analysis. Journal of Heredity. 2009;100(5):556-564. doi: 10.1093/jhered/esp028
  11. Dzhulamanov KM, Gerasimov NP, Dubovskova MP, Baktygalieva AT. Polymorphisms of CAPN1, CAST, GDF5, TG5 and GH genes in Russian Hereford cattle. Bulgarian Journal of Agricultural Science. 2019;25(2):375-379.
  12. Groeneveld LF, Lenstra JA, Eding H, Toro MA, Scherf B, Pilling D, Negrini R, Finlay EK, Jianlin H, Groeneveld E, Weigend S. Genetic diversity in farm animals – a review. Anim Genet. 2010;41(s1):6-31.doi: 10.1111/j.1365-2052.2010.02038.x
  13. Lee J-H, Lee Y-M, Lee J-Y, Oh D-Y, Jeong D-J, Kim J-J. Identification of single nucleotide polymorphisms (SNPs) of the bovine growth hormone (bGH) gene asociated with growth and carcass traits in hanwoo. Asian Australas. J Anim Sci. 2013;26(10):1359-1364. doi: 10.5713/ajas.2013.13248
  14. Sedykh TA, Gizatullin RS, DolmatovaIYu, Gusev IV, Kalashnikova LA. Growth hormone gene polymorphism in relation to beef cattle carcass quality. Russian Agricultural Sciences. 2020;46(3):289-294. doi: 10.3103/S1068367420030167
  15. Smith TPL, Casas E, Rexroad CE et al. Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness. J Anim Sci. 2000;78(10):2589-2594. doi: 10.2527/2000.78102589x
  16. Tyulebaev SD, Kadysheva MD, Kosilov VI, GabidulinVM. The state of polymorphism of genes affecting 2the meat quality in micropopulations of meat Simmentals. IOP Conference Series: Earth and Environmental Science. 2021;624:012045. doi: 10.1088/1755-1315/624/1/012045

Information about the authors:

Evgenia S Surzhikova, Cand. Sci. (Agriculture), Senior Researcher, Laboratories of Immunogenetics and DNA Technologies, North Caucasus Federal Agrarian Research Center, 356241, Stavropol Territory, Shpakovsky district, Mikhailovsk, st. Nikonova, 49.

Marina P Dubovckova, Dr. Sci. (Agriculture), Leading Researcher, Breeding and Genetic Center For Beef Cattle Breeds, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, 29 9 Yanvarya St., cell.: 8-922-621-61-78.

Nikolay P Gerasimov, Dr. Sci. (Biology), Senior Researcher, Breeding and Genetic Center For Beef Cattle Breeds, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, 29 9 Yanvarya St., cell: 8-912-358-96-17.

 The article was submitted 13.10.2021; approved after reviewing 22.11.2021; accepted for publication 13.12.2021

Download