Elena V Sheyda, Vitaly A Ryazanov, Sergey A Miroshnikov, Galimzhan K Duskaev

Animal Husbandry and Fodder Production. 2021. Vol. 104, no 4. Р. 12-21.

doi: 10.33284/2658-3135-104-4-12

 Changes in the biochemical parameters of saliva, blood and the degree of feed

digestibility (in vitro) against the background of the introduction of sunflower husk to young cattle

 Elena V Sheyda1,5, Vitaly A Ryazanov2, Sergey A Miroshnikov3,6, Galimzhan K Duskaev4

1.,2,3,4Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

5,6Orenburg State Agrarian University, Orenburg, Russia

1,5elena-snejjda@mail.ru, https://orcid.org/0000-0002-2586-613X

2vita7456@yandex.ru, https://orcid.org/0000-0003-0903-9561

3,6rector_osu@mail.osu.ru, https://orcid.org/orcid.org/0000-0003-1173-1952

4gduskaev@mail.ru, https://orcid.org/orcid.org/0000-0002-9015-8367

 Abstract. The work studied the change in the biochemical parameters of individual biosubstrates (saliva, blood) of young cattle against the background of feeding indigestible production waste – sunflower husks. In the group receiving husk, there was an increase in antioxidant activity relative to the control, so in the blood serum the activity of the enzyme superoxide dismutase was significantly higher by 34%, while the lipid peroxidation index was 26% (P≤0.05). Morphological and biochemical blood tests showed that all the studied parameters of both the control and experimental groups were within the physiological norm, which is the reason to consider husk as a fodder. Biochemical analysis of saliva demonstrated in the experimental group, relative to the control diet, an increase in the activity of digestive enzymes p-amylase by 61% and lipase by 76.4% (P≤0.05), an increase in the concentration of Ca by 70.4%, Fe by 44.9% and urea by 73% (P≤0.05) was also noted. An in vitro study of ruminal digestion with the inclusion of husk and additional administration of small molecules of vanillin, 4 hexylresorcinol, salicylic acid and ultrafine particles showed an increase in the digestibility of dry matter in samples with salicylic acid and chromium UFPs, however, the mechanism of action has not yet been studied. It is necessary to conduct further research on the selection of additives, their dosages and the exposure of administration to increase the digestibility of husk and its use in feeding young cattle.

Keywords: calves, feeding, sunflower husk, small molecules, ultrafine particles of Fe, Cr2O3, digestibility, antioxidant status, digestive enzymes, morphological composition of blood, biochemical composition of blood

Acknowledgments: the work was supported by the Russian Science Foundation, Project № 20-16-00088.

For citation: Sheyda EV, Ryazanov VA, Miroshnikov SA, Duskaev GK. Changes in the biochemical parameters of saliva, blood and the degree of feed digestibility (in vitro) against the background of the introduction of sunflower husk to young cattle. Animal Husbandry and Fodder Production. 2021;104(4):12-21. (In Russ.). https://doi.org/10.33284/2658-3135-104-4-12

References

  1. Benchaar C, Calsamiglia S, Chaves AV, Fraser GR, Colombatto D, Mcallister TA, Beauchemin KA. A review of plant-derived essential oils in ruminant nutrition and production. Anim Feed Sci Technol. 2008;145(1-4):209-228. doi: 10.1016/j.anifeedsci.2007.04.014
  2. Busquet M, Calsamiglia S, Ferret A, Carro MD, Kamel C. Effect of garlic oil and four of its compounds on rumen microbial fermentation. J Dairy Sci. 2005;88(12):4393-4404. doi: 10.3168/jds.S0022-0302(05)73126-X
  3. Castillejos L., Calsamiglia S., Ferret A. Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J Dairy Sci. 2006;89:2649-2658. doi: 10.3168/jds.S0022-0302(06)72341-4
  4. Castillo-Lopez E, Petri RM, Ricci S, Rivera-Chacon R, Sener-Aydemir A, Sharma S, Reisinger N, Zebeli Q. Dynamic changes in salivation, salivary composition, and rumen fermentation associated with duration of high-grain feeding in cows. J Dairy Sci. 2021;104(4):4875-4892. doi: 10.3168/jds.2020-19142
  5. Contreras-Aguilar M, Henry S, Coste C, Tecles F, Escribano D, Cerón, JJ, Hausberger M. Changes in saliva analytes correlate with horses’ behavioural reactions to an acute stressor: a pilot study. Animals. 2019;9(11):993. doi: 10.3390/ani9110993
  6. Contreras-Aguilar MD, Lamy E, Escribano D, Cerón JJ, Tecles F, Quiles AJ, Hevia ML. Changes in salivary analytes of horses due to circadian rhythm and season: a pilot study. Animals. 2020;10(9):1486. doi: 10.3390/ani10091486
  7. Contreras-Aguilar MD, López-Arjona M, Martínez-Miró S, Escribano D, Hernández-Ruipérez F, Cerón JJ, Tecles F. Changes in saliva analytes during pregnancy, farrowing and lactation in sows: A sialochemistry approach. Vet J. 2021a;273:105679. doi: 10.1016/j.tvjl.2021.105679
  8. Contreras-Aguilar MD, Vallejo-Mateo PJ, Lamy E, Escribano D, Cerón JJ, Tecles F, Rubio CP. Changes in saliva analytes in dairy cows during peripartum: a pilot study. Animals. 2021b;11(3):749. doi: 10.3390/ani11030749
  9. Deryabin D, Galadzhieva A, Kosyan D, Duskaev G. Plant-derived inhibitors of AHL-mediated quorum sensing in bacteria: modes of action. Int J Mol Sci. 2019;20(22):5588. doi: 10.3390/ijms20225588
  10. Deryabin DG, Tolmacheva АA. Antibacterial and anti-quorum sensing molecular compositionderived from Quercus cortex (oak bark) extract. Molecules. 2015;20(9):17093-17108. doi: 10.3390/molecules200917093
  11. Duodu CP, Adjei-Boateng D, Edziyie RE, Agbo NW, Owusu-Boateng G, Larsen BK, Skov PV. Processing techniques of selected oilseed by-products of potential use in animal feed: Effects on proximate nutrient composition, amino acid profile and antinutrients. Anim Nutr. 2018;4(4):442-451. doi: 10.1016/j.aninu.2018.05.007
  12. Duskaev GK, Karimov IF, Levakhin GI, Nurzhanov BS, Rysaev AF, Dusaeva KhB. Ecology of ruminal microorganisms under the influence of Quercus Cortex extract. International Journal of GEOMATE. 2019;16(55):59-66. doi: 10.21660/2019.55.4673
  13. Escribano D, Horvatić A, Contreras-Aguilar MD,  Guillemin N,  Cerón JJ,  Lopez-Arjona M, Hevia ML, Eckersall PD , Manteca X, Mrljak V. Identification  of  possible  new  salivary  biomarkers  of stress  in  sheep  using a high-resolution quantitative proteomic technique. Res Vet Sci. 2019;124:338-345.  doi: 10.1016/j.rvsc.2019.04.012
  14. Foskolos A, Ferret A, Siurana A, Castillejos L, Calsamiglia S. Effects of capsicum and propyl-propane thiosulfonate on rumen fermentation, digestion, and milk production and composition in dairy cows. Animals. 2020;10(5):859. doi: 10.3390/ani10050859
  15. Fouhse JM, Smiegielski L, Tuplin M, Guan LL, Willing BP. Host immune selection of rumen bacteria through salivary secretory IgA. Front Microbiol. 2017;8:848. doi: 10.3389/fmicb.2017.00848
  16. Hansen SL, Ashwell MS, Moeser AJ, Fry RS, Knutson MD, Spears JW. High dietary iron reduces transporters involved in iron and manganese metabolism and increases intestinal permeability in calves. J Dairy Sci. 2010;93(2):656-665. doi: 10.3168/jds.2009-2341
  17. Klingel T, Kremer JI, Gottstein V, Rajcic de Rezende T, Schwarz S, Lachenmeier DW. A review of coffee by-products including leaf, flower, cherry, husk, silver skin, and spent grounds as novel foods within the European Union. Foods. 2020;9(5):665. doi: 10.3390/foods9050665
  18. Logachev K, Karimov I, Duskaev G, Frolov A, Tulebaev S, Zav'yalov O. Study of intercellular interaction of ruminal microorganisms of beef cattle. Asian Journal of Animal Sciences. 2015;9(5):248-253. doi: 10.3923/ajas.2015.248.253
  19. MacNamara JP, Valdez F.   Adipose  tissue  metabolism  and  production  responses to  calcium  propionate  and  chromium  propionate.  J  Dairy  Sci.  2005;88(7):2498-2507. doi: 10.3168/jds.S0022-0302(05)72927-1
  20. Marcus A,  Fox G.  Fungal Biovalorization of a brewing industry byproduct, brewer's spent grain: a review. Foods. 2021;10(9):2159.  doi: 10.3390/foods10092159
  21. Mpendulo CT, Chimonyo M, Ndou SP, Bakare AG. Fiber source and inclusion level affects characteristics of excreta from growing pigs. Asian-Australas J Anim Sci. 2018a;31(5):755-762. doi: 10.5713/ajas.14.0611
  22. Mpendulo CT, Hlatini VA, Ncobela CN, Chimonyo M. Effect of fibrous diets on chemical composition and odours from pig slurry. Asian-Australas J Anim Sci. 2018b;31(11):1833-1839. doi: 10.5713/ajas.16.0126
  23. Myint H, Kishi H, Koike S, Kobayashi Y. Effect of chickpea husk dietary supplementation on blood and cecal parameters in rats. Anim Sci J. 2017;88(2):372-378. doi: 10.1111/asj.12651
  24. Núñez MT. Regulatory mechanisms  of  intestinal  iron  absorption-uncovering  of  a  fast-response  mechanism based on DMT1Regulatory mechanisms of intestinal iron absorption-uncovering of a fast-response mechanism based on DMT1 and ferroportin endocytosis. Biofactors. 2010;36(2):88-97. doi: 10.1002/biof.84
  25. Panama Varietals GmbH. [Internet] Coffee Husk (Cascara) - the dried husk of the coffee fruit or coffee cherry. Available from: https://ec.europa.eu/food/sites/food/files/safety/docs/novel-food_sum_ongoing-app_2018-0192.pdf(accessed March 20, 2020).
  26. Rodríguez-Prado M, Ferret A, Zwieten J, Gonzalez L, Bravo D, Calsamiglia S. Effects of dietary addition of capsicum extract on intake, water consumption, and rumen fermentation of fattening heifers fed a high-concentrate diet. J. Anim. Sci. 2012;90:1879-1884. doi: 10.2527/jas.2010-3191
  27. Song R, Foster DN, Shurson GC. Effects of feeding diets containing bacitracin methylene disalicylate to heat-stressed finishing pigs. J Anim Sci. 2011;89(6):1830-1843. doi: 10.2527/jas.2010-3218
  28. Tecles F, Contreras-Aguilar MD, Martínez-Miró S,  Tvarijonaviciute A,  Martínez-Subiela S, Escribano D, Cerón JJ. Total  esterase  measurement  in  saliva  of  pigs: validation  of  an  automated  assay,  characterization  and  changes  in  stress and disease conditions. Res Vet Sci. 2017;114:170-176.  doi: 10.1016/j.rvsc.2017.04.007
  29. Turzyński T, Kluska J, Ochnio M, Kardaś D. Comparative analysis  of  pelletized  and  unpelletized  sunflower  husks  combustion process in a batch-type reactor. Materials. 2021;14(10):2484. doi: 10.3390/ma14102484
  30. Zhang J, Shi HT, Wang YC, Li SL, Cao ZJ, Yang HJ, Wang YJ. Carbohydrate and amino acid metabolism and oxidative status in Holstein heifers precision-fed diets with different forage to concentrate ratios. Animal. 2020;14(11):2315-2325. doi: 10.1017/S1751731120001287

Information about the authors:

Elena V Sheyda, Cand. Sci (Biology), Researcher at the Laboratory of Biological Tests and Examinations, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, 29, 9 Yanvarya St.; Senior Researcher, Experimental Biological Clinic, 460018, Orenburg, Orenburg State University, 13 Pobedy Ave, 8-922-862-64-02.

Vitaly A Ryazanov, Cand. Sci. (Agriculture), Researcher of the Department of Feeding for Farm Animals and Feed Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79.

Sergey A Miroshnikov, Dr. Sci. (Biology), RAS Corresponding Member, Chief Researcher of the Department of Feeding for Farm Animals and Feed Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, 29, 9 Yanvarya St., tel.: 8(3532)30-81-70; Interim Rector, Orenburg State University, 460018, Orenburg, 13 Pobedy Ave, tel.:77-67-70.

Galimzhan K Duskaev, Dr. Sci. (Biology), Leading Researcher of the Department of Feeding for Farm Animals and Feed Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79.

The article was submitted 29.07.2021; approved after reviewing 17.09.2021; accepted for publication 13.12.2021.

Download