Elena V Sheyda, Victoria V Crechkina, Elena А Rusakova

Changes in morphological and biochemical parameters of rat blood with additional introduction

of zinc asparaginate into the diet

 DOI: 10.33284/2658-3135-103-2-100

UDC 599.323.4:591.11

Acknowledgements:

Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (№ 0526-2019-0001)

Changes in morphological and biochemical parameters of rat blood with additional introduction

of zinc asparaginate into the diet

Elena V Sheyda, Victoria V Crechkina, Elena А Rusakova

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Summary. Zinc is a vital element, as it is part of many important enzymes in a living organism. Along with the effective use of zinc asparaginate in animal feeding, negative effects of its influence on the functional state of some body systems are also noted. Therefore, in this study, the effect of additional introduction of zinc in various doses in the diet on hematological parameters of blood of laboratory rats was studied. An experimental study was conducted on white male Wistar rats. Zinc asparaginate was administered peros in dosages of 1.7; 3.4; 5.1 mg/kg, the drug was mixed with food, animals were on a standard diet. The zinc preparation was administered to animals of experimental groups daily for 28 days. The changes in morphological and biochemical parameters of blood were taken into account on days 14 and 28. The results of a morphological blood test showed that the introduction of Zn into the diet for 2 weeks daily contributed to a decrease in the level of hemoglobin, red blood cells, white blood cells against a background of an increase in platelets of 18.5-55.7%, depending on the dose. On day 28 of the count, an increase in the level of leukocytes by 19.6-62.2%, and platelets by 8.5-17.8% was noted. The hemoglobin level increased relative to 14 days of study, however, relative to the control, it remained lower. Biochemical analysis showed that zinc asparaginate, regardless of dosage and time period of the experimental study, contributes to an increase in the level of ALT and ACT, Tg, creatinine, uric acid, urea, alkaline phosphatase, bilirubin and total protein enzymes, with the exception of glucose alone; a decrease in this indicator in all experimental groups.

Key words: rats, zinc, asparaginates, blood, morphology, biochemistry.

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Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

Grechkina Victoria Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertise, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8-922-877-14-97, e-mail: Viktoria1985too@mail.ru

Rusakova Elena Anatolyevna, Cand. Sci. (Biol.), Senior Researcher, Laboratory for Breeding and Genetic Research in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., e-mail: elenka_rs@mail.ru

Received: 4 June 2020; Accepted: 15 June 2020; Published: 8 July 2020

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