Tatyana N Kholodilina, Tatyana A Klimova, Kristina S Kondrashova, Vladimir V Vanshin, Vladimir L Korolyov

The response of the intestinal microflora of broiler chickens to the introduction of dietary fiber in diets with different levels of calcium

DOI: 10.33284/2658-3135-103-2-133

UDC 636.5:636.085

The response of the intestinal microflora of broiler chickens to the introduction of dietary fiber

in diets with different levels of calcium

Tatyana N Kholodilina1,2, Tatyana A Klimova 1.2, Kristina S Kondrashova1.2, Vladimir V Vanshin2,

Vladimir L Korolyov1

1 Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

2Orenburg State University (Orenburg, Russia)

Summary. The article describes the results of studies evaluating the effect of extrusion (hot extrusion) of feed on the microbiome of cecum of the intestines of broiler chickens (Arbor-Acres cross) under varying dosages of calcium (CaCO3 in concentrations from 10 to 25%). A decrease in the proportion of hardly hydrolyzable carbohydrates in the diet contributed to a 5-fold decrease in the number of Escherichia relative to the control, and a decrease in the growth of Staphylococcus, Clostridium perfringens. The introduction of CaCO3 had a significant effect on the biocenosis. Intensive growth of both obligate and conditionally pathogenic microflora was noted. It was found that an increase in the concentration of calcium in the extrudate led to a significant increase – up to 1.96 * 109 CFU/g (P≤0.001) of Lactobacillus relative to the control diet. In this case, the greatest growth result relative to the control is shown by the experimental group with 20% Ca injection – 7.23 * 109 CFU / g (P≤0.001). Increasing Ca dosages to 20% leads to a maximum growth of Escherichia, 7.3 times higher than in the control group. For Staphylococcus bacteria, a dose-dependent effect was established with an increase in the concentration of CaCO3 from 10 to 20%. In general, the ability of the structure of the diet to influence the composition of the microbiome of cecum of the intestine was revealed, which in further studies will allow, using biotic relationships between bacteria, to obtain a physiological response in terms of bird productivity.

Key words: broiler chickens, feeding, intestinal microflora, cecum, extrusion, calcium, dietary fiber.

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Kholodilina Tatyana Nikolaevna, Cand. Sci (Agr.), Head of Testing Center of the Common Use Centre, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-77, e-mail: icvniims@mаil.ru Associate Professor, Departments of Ecology and Nature Management, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave, e-mail: тел.: +79128487473, e-mail: Xolodilina@rambler.ru

Klimova Tatiana Andreevna, specialist-technician of Testing Center of the Common Use Centre, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.; graduate student, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave.; tel.: +79878494166, e-mail: klimovat91@mail.ru

Kondrashova Kristina Sergeevna, Junior Researcher at the Laboratory for Breeding and Genetic Research, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.; graduate student, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave.; tel.: +79228408002, e-mail: christinakondrashova94@yandex.ru

Vanshin Vladimir Valerievich, Cand. Sci (Agr.), Associate Professor of the Department of Food Production Technology, Orenburg State University, 13 Pobedy Ave., tel.: +79123442180, e-mail: vanschin.v@mail.ru

Korolyov Vladimir Leontyevich, Dr. Sci (Agr.), Leading Researcher Department of Agricultural Animals Nutrition and Fodder Technology named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79

Received: 5 June 2020; Accepted: 15 June 2020; Published: 8 July 2020

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