Aminova E.V, Mushinsky A.A, Saudabaeva A.Zh.
Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0011)
Stress tolerance of Solanum tuberosum under the influence of silicon dioxide UFPs
Evgenia V Aminova, AlexanderA Mushinsky, Alia Zh Saudabaeva
Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)
Summary. Interest in the use of ultrafine particles in crop production and agricultural practice is associated primarily with their unique properties. The presented work evaluates the impact of ultrafine particles of silicon dioxide in various concentrations on Solanum tuberosum. The objects of the study were potato tubers of the "Tarasov" variety and silicon dioxide UFPs. The samples were treated with a suspension of SiO2 UFPs at concentrations: 0.03, 0.09, 0.18, 0.21, and 0.36 g / kg of potatoes. During the experiment, the peroxidase activity (according to Boyarkin's method), cell viability according to the manufacturer's protocol (WST-8 patent No. 2.251.850, Canada) and the integrity of the DNA molecules of Solanum tuberosum plants (from plant cells by phenol-chloroform extraction) were determined. According to the results of the study, nanosilica at a concentration of 0.21 and 0.36 g/kg had a statistically significant effect on the number of living cells (there was a decrease to 17.1%). It was revealed that the silicon dioxide UFPs at a concentration of 0.09 and 0.18 g/kg had a significant effect on the peroxidase activity in Solanum tuberosum plants. The results of the studies carried out confirmed the decrease in the intensity of the DNA luminosity peaks by a temporally calculated linear profile at a maximum dilution of 0.36 g/kg.
Key words: potato, silicon dioxide, nanoparticles, potato stress resistance, cell viability, integrity of DNA molecules.
- Alechin NE, Avakyan AP, Alechin EP. Si content in rice DNA. Dokl. VASKHNIL. 1982;6:6-7.
- Maslobrod SN, Mirgorod YuA, Borodina VG, Borshch NA. Influence of water dispersed systems with silver and copper nanoparticles on seed germination. Electronic Processing of Materials. 2014;50(4):103-112.
- Volodina LA, Zhigach AN, Leypunsky IO, Zotova ES, Glushchenko NN. The influence of physical-chemical characteristics of surface modified copper nanoparticles on E. coli cell population growth suppression and on electrostatic properties of their membranes. Biophysics. 2013;58(3):394-401.
- Voronkov MG, Zelchan GI, Lukevits EYa. Silicon and Life: Biochemistry, Pharmacology and Toxicology of Silicon Compounds. 2nd ed., rev. and add. Riga: Zinatne;1978:587 p.
- Matychenkov IV. Mutual influence of silicon, phosphoric and nitrogen fertilizers in the soil-plant system. [dissertation] Moscow; 2014:136 p.
- Korotkova AM, Lebedev SV, Kvan OV, Atlanderova KN. Evaluation of ultrafine particles influence on biometric parameters of wheat germs. Animal Husbandry and Fodder Production. 2019;102(2):7-20. doi: 10.33284/2658-3135-102-2-7
- Belanger MC, Ouellet M, Queney G, Moreau M. Taurine-deficient dilated cardiomyopathy in a family of golden retrievers. J Am Anim Hosp Assoc. 2005;41(5):284-291. doi: 10.5326/0410284
- Dimkpa CO, McLean JE, Latta DE, Manangón E, Britt DW, Johnson WP, Boyanov MI, Anderson AJ. CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat. J Nanopart Res. 2012;14:1125. doi: https://doi.org/10.1007/s11051-012-1125-9
- Hassan E, Byoung RJ. Silicon (Si): Review and future prospects on the action mechanisms in alleviating biotic and abiotic stresses in plants. Ecotoxicology and Environmental Safety. 2018;147:881-896. doi: https://doi.org/10.1016/j.ecoenv.2017.09.063
- Lei C, Sun Y, Tsang DCW, Lin D. Environmental transformations and ecological effects of iron-based nanoparticles. Environmental Pollution. 2018;232:10-30. doi: https://doi.org/10.1016/j.envpol.2017.09.052
- Lopes S, Pinheiro C, Soares AMVM, Loureiro S. Joint toxicity prediction of nanoparticles and ionic counterparts: Simulating toxicity under a fate scenario. Journal of Hazardous Materials. 2016;320:1-9. doi: https://doi.org/10.1016/j.jhazmat.2016.07.068
- Ma C, Liu H, Guo H, Musante C, Coskun SH, Nelson BC, White JC, Xing B, Dhankher OP. Defense mechanisms and nutrient displacement in Arabidopsis thaliana upon exposure to CeO2 and In2O3 nanoparticles. Environ Sci: Nano. 2016;3:1369-1379. doi: http://doi:10.1039/C6EN00189K
- Rastogi A, Tripathi DK, Yadav S et al. Application of silicon nanoparticles in agriculture. Biotech. 2019;9:90. doi: https://doi.org/10.1007/s13205-019-1626-7
- Rizwan M, Ali S, Qayyum MF, Ok YS, Adrees M, Ibrahim M, Zia-ur-Rehman M, Farid M, Abbas F. Effect of metal and metal oxide nanoparticles on growth and physiology of globally important food crops: A critical review. J Hazard Mater. 2017;322 (Part A):2-16. doi: https://doi.org/10.1016/j.jhazmat.2016.05.061
- Roohizadeh G, Arbabian S, Tajadod G, Majd A, Salimpour F. The study of Nano silica effects on the total protein content and the activities of catalase, peroxidase and superoxid dismutase of Vicia faba L. Trop Plant Res. 2015;2(1):47-50.
- Singh D, Kumar A. Investigating long-term effect of nanoparticles on growth of Raphanus sativus plants: a trans-generational study. Ecotoxicology. 2018;27(1):23-31. doi: https://doi.org/10.1007/s10646-017-1867-3
- Tripathi DK, Shweta, Singh S, Singh S, Pandey R, Singh VP, Sharma NC, Prasad SM, Dubey NK, Chauhan DK. An overview on manufactured nanoparticles in plants: Uptake, translocation, accumulation and phytotoxicity. Plant Physiology and Biochemistry. 2017;110:2-12. doi: 10.1016/j.plaphy.2016.07.030
Aminova Evgenia Vladimirovna, Cand. Sci. (Agr.), Leading Researcher, Potato Department, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460051, 27/1 Gagarina Ave., Orenburg, Russia, 27/1 Gagarina Ave., tel.: 8-912-841-19-31, e-mail: email@example.com
Mushinsky Alexander Alekseevich, Dr. Sci. (Agr.), Leading Researcher, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel: 8-905-819-35-92, e-mail: firstname.lastname@example.org
Saudabaeva Alia Zhonysovna, Cand. Sci. (Biol.), Potato Department, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460051, Orenburg, Russia, 27/1 Gagarina Ave., tel.: 89128411931, e-mail: email@example.com
Received: 3 September 2020; Accepted: 14 September 2020; Published: 30 September 2020Download