Azhmuldinov E.A, Titov M.G, Kizaev M.A, Kholodilina T.N.

DOI: 10.33284/2658-3135-103-3-145

UDC 636.92:577.17:591.11


Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0006)

Influence of ultrafine silver particles on morphobiochemical parameters of animals

under heat stress

Elemes A Azhmuldinov, Maxim G Titov, Mikhail A Kizaev, Tatyana N Kholodilina

Federal Research Centre of Biological Systems and Agrotechnologies of Russian Academy of Sciences (Orenburg, Russia)

Summary. Heat stress has a negative impact on health and productivity of animals, causing huge economic losses. An alternative to the existing approaches to minimize the negative effect of stress factors can be the use of ultrafine particles (UFP) of metals, in particular silver. The aim of the research was to study the dose-dependent effect of silver UFPs on morphobiochemical composition of blood of animals at high temperatures. The experiment was carried out on 5-month male rabbits of the Giant breed under standard vivarium conditions. Three groups were formed: control and two experimental. Rabbits of all groups were exposed to heat (exposure for three hours at t + 42 °C and air humidity 75%). The animals of the experimental groups were injected intramuscularly with a suspension of silver UFP in catholyte daily, seven days before the start of heat exposure, in doses: Group I - 0.01, II - 0.05 mg/kg of live weight.

Correction of heat stress with a suspension of silver UFP in catholyte led to a decrease in some blood parameters. In particular, hematocrit, hemoglobin, leukocytes and platelets tended to decrease compared to the control group with the severity of the effect at a low dose of 0.01 mg/kg body weight. The activity of serum transferases: gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) remained high in the absence of heat stress correction (control group) and significantly (P≤0.01) decreased with the use of a low dose (Group I).

Thus, the use of a suspension of silver UFP in catholyte at a dose of 0.01 mg / kg of live weight improves the protective properties of body and has a protective effect against heat stress.

Key words: rabbits, silver UFP, blood, serum, gamma-glutamyltransferase, alanine aminotransferase, aspartate aminotransferase, heat stress.


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Azhmuldinov Elemes Azhmuldinovich, Dr. Sci (Agr.), Professor, Chief Researcher, Department of Technology of Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78

Titov Maxim Gennadevich, Cand. Sci (Agr.), Senior Researcher, Department of Technology of Beef Cattle Breeding and Beef Production, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-78, e-mail:

Kizaev Mikhail Anatolevich, Cand. Sci (Agr.), Scientific Secretary, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-73, e-mail:

Kholodilina Tatyana Nikolaevna, Cand. Sci (Agr.), Head of Testing Center of the Common Use Centre, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-77, e-mail: icvniims@mа

Received: 2 September 2020; Accepted: 14 September 2020; Published: 30 September 2020