Duskaev G.K, Levakhin G.I, Dokina N.N.

DOI: 10.33284/2658-3135-103-3-204

UDC 636:615.32


Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0005)

Medicinal plants and their use in animal husbandry

Galimzhan K Duskaev, Georgy I Levakhin, Nina N Dokina

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Summary. The relevance of the studied research problem is determined by the global awareness of the need to reduce the use of feed antibiotics in animal husbandry and by the development of alternative approaches to control bacterial infections in farm animals. It is confirmed by the adoption of the "Strategy for preventing the spread of antimicrobial resistance in the Russian Federation until 2030" (September 2017 - Order of the Government of the Russian Federation No. 2045-r) and the first surveillance data on antimicrobial resistance (January 2018 - report of the World Health Organization ), indicating high levels of antibiotic resistance for a number of serious bacterial infections in both high- and low-income countries. At present, the EU is already spending 1.5 billion euros per year on treatment of resistant pathogens; in the United States, the cost of managing antibiotic resistance is 0.1-10 billion USD per year. According to the WHO, in the next 35 years, about 300 million people are expected to die due to antibiotic resistance. By 2050, antibiotic resistance could lead to a decrease in global GDP by 2-3.5% and damage of $ 100 trillion. In this regard, the search for alternative substances - analogs to feed antibiotics is an urgent task, since the results will correspond to the world level of research, which is confirmed by the intensification of research by the world's leading scientific laboratories specifically on phytochemicals, emphasizing their potential, as well as the formation of world priorities for the use of various phytochemicals for obtaining agricultural products as alternatives to antibiotics (December 2016 - 2nd International Symposium on Alternatives to Antibiotics, headquarters of the World Organization for Animal Health (OIE), Paris, France).

Key words: farm animals, productivity, herbal extracts, immunity, digestion, antibiotic.


  1. Ayrle H, Mevissen M,  Kaske M,  Nathues H,  Gruetzner N,  Melzig M and  Walkenhorst M. Medicinal plants - prophylactic and therapeutic options for gastrointestinal and respiratory diseases in calves and piglets? A systematic review. BMC Vet Res. 2016;12:89. doi: 10.1186/s12917-016-0714-8
  2. Bazargani MM, Rohloff J. Antibiofilm activity of essential oils and plant extracts against Staphylococcus Aureus and  Escherichia   Coli biofilms. Food Microbiol. 2016;61:156-164. doi: https://doi.org/10.1016/j.foodcont.2015.09.036
  3. Berger LM, Wein S, Blank R, Metges CC, Wolffram S. Bioavailability of the flavonol quercetin in cows after intraruminal application of quercetin aglycone and rutin. J DairySci. 2012;95(9):5047-5055. doi: https://doi.org/10.3168/jds.2012-5439
  4. Betts JW, Hornsey M, La Ragione RM. Novel antibacterials: alternatives to traditional antibiotics. Advances in Microbial Physiology. 2018;73:123-169. doi: https://doi.org/10.1016/bs.ampbs.2018.06.001
  5. Bridi R, Giordano A, Peñailillo MF, Montenegro G. Antioxidant effect of extracts from native chilean plants on the lipoperoxidation and protein oxidation of bovine muscle. Molecules. 2019;24(18): 3264. doi: https://doi.org/10.3390/molecules24183264
  6. Brown DG, Lister T, May-Dracka TL. New natural products as new leads for antibacterial drug discovery. Bioorganic Med Chem Lett. 2014;24(2):413-418. doi: https://doi.org/10.1016/j.bmcl.2013.12.059
  7. Bruins MJ, Vente-Spreeuwenberg MAM, Smits CH, Frenken LGJ. Black tea reduces diarrhoea prevalence but decreases growth performance in enterotoxigenic Escherichia coli-infected post-weaning piglets. J Anim Physiol Anim Nutr. 2011;95(3):388-398. doi: 10.1111/j.1439-0396.2010.01066.x
  8. Chakraborty S, Dutta TK, De A, Das M, Ghosh S. Impact of bacterial biofilm in veterinary medicine: an overview. Int J Curr Microbiol App Sci. 2018;7(4):3228-3239. doi: https://doi.org/10.20546/ijcmas.2018.704.366
  9. Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing new antimicrobial therapies: are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacogn Rev. 2017;11(22):57-72. doi: 10.4103/phrev.phrev_21_17
  10. Das MC, Sandhu P, Gupta P, Rudrapaul P, De UC, Tribedi P, Akhter Y, Bhattacharjee S. Attenuation of Pseudomonas aeruginosabiofilm formation by Vitexin: A combinatorial study with azithromycin and gentamicin. Sci Rep. 2016;6:23347. doi: 10.1038/srep23347
  11. De la Fuente-Núñez C, Cardoso MH, De Souza Candido E, Franco Ol, Hancock REW. Synthetic antibiofilm peptides.   Biochim   Biophys   Acta   Biomembr. 2016;1858(5):1061-1069. doi: https://doi.org/10.1016/j.bbamem.2015.12.015
  12. De la Fuente-Núñez C, Korolik V, Bains M, Nguyen U, Breidenstein EBM, Horsman S, Lewenza S, Burrows L, Hancock REW. Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. Antimicrobial Agents and Chemotherapy. 2012;56(5):2696-2704. doi: 10.1128/AAC.00064-12
  13. De Sousa OA,  Cooke RF,  Brandão AP,  Schubach KM,  Schumaher TF,  Bohnert DW, Marques RS. Productive and physiological responses of feeder cattle supplemented with Yucca schidigera extract during feedlot receiving. J Anim Sci. 2019;97(1):208-219. doi:  https://doi.org/10.1093/jas/sky412
  14. De Souza KA, Cooke RF, Schubach KM, Brandão AP, Schumaher TF, Prado IN, Marques RS, Bohnert DW. Performance, health and physiological responses of newly weaned feedlot cattle supplemented with feed-grade antibiotics or alternative feed ingredients. Animal. 2018;12(12):2521-2528. doi: 10.1017/S1751731118000551
  15. Dijkstra J, Oenema O, Bannink A. Dietary strategies to reducing N excretion from cattle: implications for methane  emissions.  Curr  Opin  Environm Sustain. 2011;3(5):414-422. doi: https://doi.org/10.1016/j.cosust.2011.07.008
  16. Disler M, Ivemeyer S, Hamburger M, Vogl CR, Tesic A, Klarer F, Meier B, Walkenhorst M. Ethnoveterinary herbal remedies used by farmers in four north-eastern Swiss cantons (St. Gallen, Thurgau, Appenzell Innerrhoden   and   Appenzell  Ausserrhoden). J  Ethnobiol  Ethnomed. 2014;10:32. doi: 10.1186/1746-4269-10-32
  17. Elisha IL, Botha FS, McGaw LJ, Eloff JN. The antibacterial activity of extracts of nine plant species with good activity against Escherichia Coliagainst five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med. 2017;17(1):133. doi: 10.1186/s12906-017-1645-z
  18. Eloff JN, McGaw LJ. Application of plant extracts and products in veterinary medicine. New Strategies Combating Bacterial Infection. Ahmad I, Aqil F, editors. Weinheim: WILEY-VCH Verlag GMBH & Co.KGaA; 2009:205-228.
  19. Famuyide IM, Aro AO, Fasina FO, Eloff JN, McGaw LJ. Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices. BMC Complement Altern Med. 2019a;19:141. doi: 10.1186/s12906-019-2547-z
  20. Famuyide IM, Aro AO, Fasina FO, Eloff JN, McGaw LJ. Antibacterial activity and mode of action of acetone crude leaf extracts of under-investigated Syzygium and Eugenia (Myrtaceae) species on multidrug resistant porcine   diarrhoeagenic   Escherichia   coli.   BMC   Vet  Res. 2019b;15(1):162. doi: 10.1186/s12917-019-1914-9
  21. Hanieh H, Narabara K, Piao M, Gerile C, Abe A, Kondo Y. Modulatory effects of two levels of dietary Alliums on immune response and certain immunological variables, following immunization, in White Leghorn chickens. Anim Sci J. 2010;81(6):673-680. doi: 10.1111/j.1740-0929.2010.00798.x
  22. Hashemi SR, Davoodi H. Herbal plants and their derivatives as growth and health promoters in animal nutrition. Vet Res Commun. 2011;35(3):169-180. doi: 10.1007/s11259-010-9458-2
  23. Hoelzer K, Wong N, Thomas J, Talkington K, Jungman E, Coukell A. Antimicrobial drug use in food-producing animals and associated human health risks: what, and how strong, is the evidence? BMC Vet Res. 2017;13(1):211. doi: https://doi.org/10.1186/s12917-017-1131-3
  24. Jamal M, Ahmad W, Andleeb S, Jalil F, Imran M, Nawaz MA, Hussain T, Ali M, Rafiq M, Kamil MA. Bacterial   biofilm   and   associated   infections.   J   Chin  Med  Assoc. 2018;81(1):7-11. doi: https://doi.org/10.1016/j.jcma.2017.07.012
  25. Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pac J Trop Biomed. 2012;2(8):597-601. doi: 10.1016/S2221-1691(12)60104-X
  26. Lebeaux D, Ghigo J.-M, Beloin C. Biofilm-related infections: bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics. Microbiol Mol Biol Rev. 2014;78(3):510-543. doi: 10.1128/MMBR.00013-14
  27. Letsididi KS, Lou Z, Letsididi R, Mohammed K, Maguy BL. Antimicrobial and antibiofilm effects of trans-cinnamic acid nanoemulsion and its potential application on lettuce. Lwt. 2018;94:25-32. doi: https://doi.org/10.1016/j.lwt.2018.04.018
  28. Li C, Lin G, Zuo Z. Pharmacological effects and pharmacokinetics properties of Radix Scutellariaeand its bioactive flavones. Biopharm Drug Dispos. 2011;32:427-445. doi: 10.1002/bdd.771
  29. Lippolis KD,  Cooke RF,  Schumaher T,  Brandão AP,  Silva LGT,  Schubach KM,  Marques RS,  Bohnert DW. Physiologic, health, and performance responses of beef steers supplemented with an immunomodulatory feed ingredient during feedlot receiving. Journal of Animal Science. 2017;95(11):4945-4957. doi: https://doi.org/10.2527/jas2017.1837
  30. Li-weber M. New therapeutic aspects of flavones: the anticancer properties of  Scutellariaand its main  active  constituents  wogonin,  baicalein  and  baicalin. Cancer  Treat  Rev. 2009;35(1):57-68. doi: 10.1016/j.ctrv.2008.09.005
  31. Lowrence RC, Ramakrishnan A, Sundaramoorthy NS, Shyam A, Mohan V, Subbarao HMV, Ulaganathan V, Raman T, Solomon A, Nagarajan S. Norfloxacin salts of carboxylic acids curtail planktonic and biofilm  mode  of  growth  in ESKAPE pathogens. J Appl Microbiol. 2018;124(2):408-422. doi: https://doi.org/10.1111/jam.13651
  32. Maass N, Bauer J, Paulicks BR, Bohmer BM, Roth-Maier DA. Efficiency of Echinacea purpurea on performance  and  immune  status  in  pigs. J  Anim  Physiol Anim Nutr. 2005;89(7-8):244-252. doi: 10.1111/j.1439-0396.2005.00501.x
  33. Macia MD, Del Pozo JL, Díez-Aguilar M, Guinea J. Microbiological diagnosis of biofilm-related infections. Enferm Infec Micr Cl. 2018;36(6):375-381. doi: https://doi.org/10.1016/j.eimc.2017.04.006
  34. Mayer M, Vogl CR, Amorena M, Hamburger M, Walkenhorst M. Treatment of organic livestock with medicinal plants: a systematic review of European ethnoveterinaryresearch. Forschende Komplementärmedizin. 2014;21(6):375-386. doi: 10.1159/000370216
  35. McSweeney CS, Palmer B, McNeill DM, Krause DO. Microbial interactions with tannins: nutritional consequences  for  ruminants. Anim Feed Sci Technol. 2001;91:83-93. doi: https://doi.org/10.1016/S0377-8401(01)00232-2
  36. Mello CP, Santos SC. Taninos. Farmacognosia: da planta ao medicamento, 4 ed. Porto Alegre, Florianópolis: Editora Universitária, UFRGS; 2002: 950 p.
  37. Meriga B, Mopuri R, MuraliKrishna T. Insecticidal, antimicrobial and antioxidant activities of bulb extracts of Allium sativum. Asian Pac J Trop Med. 2012;5(5):391-395. doi: 10.1016/S1995-7645(12)60065-0
  38. Mordmuang A, Voravuthikunchai SP. Rhodomyrtus tomentosa(Aiton) Hassk. leaf extract: an alternative approach for the treatment of staphylococcal bovine mastitis. Res Vet Sci. 2015;102:242-246. doi: 10.1016/j.rvsc.2015.07.018
  39. Mudzengi CP, Murwira A, Tivapasi M, Murungweni C, Burumu JV and Halimani T. Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management. Pharm Biol. 2017; 55(1): 1054-1060. doi: 10.1080/13880209.2017.1287744
  40. O’Neill W, McKee S, Clarke AF. Immunological and haematinic consequences of feeding a standardised Echinacea (Echinacea angustifolia) extract to healthy horses. Equine Vet J. 2002;34(3):222-227. doi: 10.2746/042516402776186001
  41. Ogbole OO, Segun PA, Fasini PS. Antimicrobial and antiprotozoal activities of twenty-four Nigerian medicinal plant extracts. S Afr J Bot. 2018;117:240-246. doi: https://doi.org/10.1016/j.sajb.2018.05.028
  42. Olagaray KE, Brouk MJ,  Mamedova LK,  Sivinski SE,  Liu H,  Robert F,  Dupuis E,  Zachut M, Bradford BJ.Dietary supplementation of Scutellaria baicalensis extract during early lactation decreases milk somatic cells and increases whole lactation milk yield in dairy cattle. PLoS One. 2019;14(1): e0210744. doi: 10.1371/journal.pone.0210744
  43. Pereira AV, Silva VA, Freitas AFR, Pereira MSV, Trevisan LFA, Costa MRM. Extratos vegetais: atividade antimicrobiana e genética sobre plasmídios de resistência a antibióticos em microrganismos. Rev Biol Farmac. 2010;4:60-65.
  44. Petruzzi B, Dalloul RA, LeRoith T, Evans NP, Pierson FW, Inzana TJ. Biofilm formation and avian immune response following experimental acute and chronic avian cholera due to Pasteurella Multocida. Vet Microbiol. 2018;222:114-123. doi: 10.1016/j.vetmic.2018.07.005
  45. Quiles AM, Fernandez-Arche MA, Garcia-Gimenez MD, De La Puerta R. Potential therapeutic applications of the genus annona: local and traditional uses and pharmacology. J Ethnopharmacol. 2018;225:244-270. doi: 10.1016/j.jep.2018.06.014
  46. Ribeiro IC de O, Mariano EGA, Careli RT, Morais-Costa F, de Sant’anna FM, Pinto MS, de Souza MR, Duarte ER. Plants of the cerrado with antimicrobial effects against Staphylococcusspp. and Escherichia colifrom cattle. Bmc Vet Res. 2018;14:32. doi: 10.1186/s12917-018-1351-1
  47. Ribeiro IC de O,  Mariano EGA,  Careli RT,  Morais-Costa F,  de Sant’Anna FM,  Pinto MS,  de Souza MR, Duarte ER. Plants of the Cerrado with antimicrobial effects against Staphylococcusspp. and Escherichia colifrom cattle. BMC Vet Res. 2018;14:32. doi: 10.1186/s12917-018-1351-1
  48. Robert F., Leboeuf L., Dupuis E. Scutellaria baicalensis extract improves milk production in dairy cows. 5th Annual Meeting of EAAP. Copenhagen; 2014: 372 p.
  49. Romulo A, Zuhud EAM, Rondevaldova J, Kokoska L. Screening of in vitro antimicrobial activity of plants used in traditional Indonesian medicine. Pharm Biol. 2018;56(1):287-293. doi: 10.1080/13880209.2018.1462834
  50. Samoilova Z, Smirnova G, Muzyka N, Oktyabrsky O. Medicinal plant extracts variously modulate susceptibility of Escherichia colito different antibiotics. Microbiol Res. 2014;169(4):307-313. doi: 10.1016/j.micres.2013.06.013
  51. Sampimon OC, Lam TJGM, Mevius DJ, Schukken YH, Zadoks RN. Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine milk samples. Vet Microbiol. 2011;150(1-2):173-179. doi: 10.1016/j.vetmic.2011.01.017
  52. Schmid K, Ivemeyer S, Vogl C, Klarer F, Meier B, Hamburger M, Walkenhorst M. Traditional use of herbal remedies in livestock by farmers in 3 Swiss cantons (Aargau, Zurich, Schaffhausen). Forsch Komplementmed. 2012;19(3):125-136. doi: https://doi.org/10.1159/000339336
  53. Sinz S, Kunz C, Liesegang A et al. In vitrobioactivity of various pure flavonoids in ruminal fermentation, with  special  reference  to  methane  formation. Czech  J  Anim  Sci. 2018;63:293-304. doi: https://doi.org/10.17221/118/2017-CJAS
  54. Sinz S,  Marquardt S,  Soliva CR,  Braun U,  Liesegang A, Kreuzer M. Phenolic plant extracts are additive in their effects against in vitro ruminal methane and ammonia formation. Asian-Australas J Anim Sci. 2019;32(7):966-976. doi: 10.5713/ajas.18.0665
  55. Stoldt A, Derno M, Nürnberg G, Weitzel JM, Otten W et al. Effects of a 6-wk intraduodenal supplementation with quercetin on energy metabolism and indicators of liver damage in periparturient dairy cows. J Dairy Sci. 2015;98(7): 4509-4520. doi: https://doi.org/10.3168/jds.2014-9053
  56. Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M, Monnet DL, Pulcini C, Kahlmeter G, Kluytmans J, Carmeli Y et al. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis. 2018;18(3):318-327. doi: 10.1016/S1473-3099(17)30753-3
  57. Tedesco D, Tava A, Galletti S, Tameni M, Varisco G, Costa A et al. Effects of silymarin, a natural hepatoprotector, in   periparturient   dairy   cows. J   Dairy   Sci. 2004;87(7):2239-2247. doi: 10.3168/jds.S0022-0302(04)70044-2
  58. Toyang NJ, Wanyama J, Nuwanyakpa M, Django S. Ethnoveterinary medicine: a practical approach to the treatment of cattle diseases in sub-Saharan Africa, 2 ed. Netherlands: Agromisa Foundation and CTA; 2007:87 p.
  59. Ushimaru PI, Barbosa LN, Fernandes AAH, Di Stasi LC, Fernandes AJ. In vitro antibacterial activity of medicinal plant extracts against Escherichia coli strains from human clinical specimens and interactions with antimicrobial drugs. Nat Prod Res. 2012;26(16):1553-1557. doi: 10.1080/14786419.2011.568943
  60. Wang HZ, Yu CH, Gao J, Zhao GR. Effects of processing and extracting methods on active components in Radix Scutellariaeby HPLC analysis. Zhongguo Zhong yao za zhi. 2007;32(16):1637-1640.
  61. Winkler A, Gessner DK, Koch C, Romberg F-J, Dusel G, Herzog E et al. Effects of a plant product consisting of green tea and curcuma extract on milk production and the expression of hepatic genes involved in endoplasmic stress response and inflammation in dairy cows. Arch Anim Nutr. 2015;69:425-441. doi: 10.1080/1745039X.2015.1093873
  62. Yan L, Kim IH. Effects of dietary supplementation of fermented garlic powder on growth performance, apparent total tract digestibility, blood characteristics and faecal microbial concentration in weanling pigs. J Anim Physiol Anim Nutr. 2013;97(3):457-464. doi: 10.1111/j.1439-0396.2012.01286.x
  63. Yelin I, Kishony R. Antibiotic Resistance. Cell. 2018;172(5):1136-1136.e1. doi: 10.1016/j.cell.2018.02.018
  64. Zanchi R, Canzi E, Molteni L, Scozzoli M. Effect of Camellia sinensis L. whole plant extract on piglet intestinal ecosystem. Ann Microbiol. 2008;58(1):147-152. doi: 10.1007/BF03179459
  65. Zhu N, Wang J, Yu L, Zhang Q, Chen K and Liu B. Modulation of growth performance and intestinal microbiota in chickens fed plant extracts or virginiamycin. Front Microbiol. 2019;10:1333. doi:10.3389/fmicb.2019.01333

Duskaev Galimzhan Kalikhanovich, Dr. Sci. (Biol.), Head of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8(3532)30-81-79, e-mail: gduskaev@mail.ru

Levakhin Georgy Ivanovich, Dr. Sci. (Biol.), Professor, Chief Researcher of the Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences», 460000, Russia, Orenburg, ul. 9 Yanvarya, 29, tel.: 8(3532)30-81-79

Dokina Nina Nikolaevna, Researcher, Farm Animal Feeding and Feed Technology Department named after Leushin SG, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Januarya St, tel.: 8(3532)30-81-79, е-mail: ndokina@mail.ru

Received: 20 Аugust 2020; Accepted: 14 September 2020; Published: 30 September 2020