Nikolay P Gerasimov, Vladimir I Kolpakov, Kinispay M Dzhulamanov, Alexandra A Lapshina

DOI: 10.33284/2658-3135-103-4-96

UDC 636.082

Acknowledgements:

Research was carried out according the plan of research scientific works on 2019-2021 yy. FSBSI FRC BST RAS (No 0761-2019-0009)

Influence of single nucleotide polymorphisms LEP C528T and LEP C73T of the leptin gene

on the assessment of the quality of carcasses and the yield of meat cuts in the Angus cows

and heifers

Nikolay P Gerasimov, Vladimir I Kolpakov, Kinispay M Dzhulamanov, Alexandra A Lapshina

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

Summary. The most important task of beef cattle breeding is the identification of molecular markers associated with the growth and development, the formation of beef productivity of cattle, which can be used in selection and breeding work. The aim of the research was to study the relationship between single nucleotide polymorphisms LEP C528T and LEP C73T of the leptin gene to assess the quality of carcasses and the yield of meat cuts in cows and heifers of the Angus breed. The studies were carried out at the feedlot and meat processing plant of the Zarechnoye Group of Companies on the livestock of cows (n = 30) after the first calving and heifers (n = 50) at the age of 20 months. Genotyping of animals taking into account the polymorphisms LEP C528T and LEP C73T of the leptin gene showed an uneven distribution of carcasses by categories depending on the genotype. The maximum proportion (16.7%) of carcasses of the highest rating "Prime" was obtained after slaughter of heifers with the LEPCC genotype (C528T), the excess relative to other gene variants was 4.2-8.3%. In the case of polymorphism in the region of the second exon of LEP C73T, the most desirable genotype of the leptin gene is LEPTT. Carcasses of the highest category "Prime" were 1.5% more frequent among carriers of this variant of the leptin gene. However, the maximum amount of the most valuable carcasses was obtained by slaughtering animals with heterozygous genotypes for the studied polymorphisms of the leptin gene. The "Prime" and "Top Choice" grades were assigned to 66.7% and 58.8% of the carcasses in the case of the LEP C528T and LEP C73T nucleotide substitutions, respectively. Thus, nucleotide substitutions in the sequence of the leptin gene LEP C528T and LEP C73T are associated with the formation of marbling and the yield of meat cuts in the Angus cows and heifers.

Key words: cow, heifer, Angus breed, MAS selection, genotype, single nucleotide polymorphism, leptin gene, meat quality, meat productivity.

References

  1. Salikhov AA, Kosilov VI, Buravov AF, Nikonova YeA. Aged changes in absolute weight of muscles in cattle young stock of Simmental breed. Vestnik of the Russia Agricultural Sciences. 2013;2:63-65.
  2. Makayev ShA, Taiguzin RSh, Lyapin OA, Fomin AV. Genetic characteristic of the Kazakh White-Headed cattle. Izvestiya of Orenburg State Agrarian University. 2019;6(80):281-285
  3. Larionova PV. Development and experimental testing of systems for the analysis of polymorphism of candidate genes for lipid metabolism in cattle: author. dis. ... Cand. biol. sciences. Dubrovitsy; 2006:23 p.
  4. Afanasyeva E, Legoshin G, Mogilenets O, Sus I, Mittelshtein T. Metodological principles of beef productivity evaluation of cattle and meat quality. Dairy and Beef Cattle Breeding. 2012;7:6-8
  5. Sermyagin AA, Bykova OA, Loretts OG, Kostyunina OV, Zinovieva NA. Genomic variability assess for breeding traits in holsteinizated Russian Black-and-White cattle using GWAS analysis and ROH patterns. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]. 2020;55(2):257-274. doi: 10.15389/agrobiology.2020.2.257eng
  6. Kostyunina OV, Abdelmanova AS, Martynova EU, Zinovieva NA. Search for genomic regions carrying the lethal genetic variants in the Duroc pigs. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology]. 2020;55(2):275-284. doi: 10.15389/agrobiology.2020.2.275eng
  7. Selionova MI, Chizhova LN, Surzhikova ES, Podkorytov NA, Podkorytov AT. Polymorphism of CAST, GH, GDF9 genes of Gorno-Altai sheep breed. Siberian Herald of Agricultural Science. 2020;50(1):92-100. doi: 10.26898/0370-8799-2020-1-11
  8. Rusakova EA, Kosyan DB. Relationship of LEP/A80V gene polymorphism with hematological parameters and characteristic of nonspecific immunity of cattle. Bulletin of the Orenburg Research Centre of the Ural Branch of the Russian Academy of Sciences. 2019;4:10. doi: 10.24411/2304-9081-2019-14027
  9. Legoshin GP, Mogilenets ON, Afanaseva ES, Mittelshtein TM. Classification of high-quality beef. Dairy and Beef Cattle Breeding. 2014;3:2-3.
  10. Baile CA, Della-Fera MA, Martin RJ. Regulation of metabolism and body fat mass by leptin. Annu Rev Nutr. 2000;20:105-127. doi: 10.1146/annurev.nutr.20.1.105
  11. Buchanan FC, Fitzsimmons CJ, Van Kessel AG, Thue TD, Winkelman-Sim DC, Schmutz SM. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet Sel Evol. 2002; 34(1):105-116. doi: 10.1051/gse:2001006
  12. Davis GP, DeNise SK. The impact of genetic markers on selection. Journal of Animal Science. 1998;76(9):2331-2339. doi: 10.2527/1998.7692331x
  13. Dekkers JCM. Commercial application of marker- and gene-assisted selection in livestock: Strategies and lessons. Journal of Animal Science. 2004;82(13):E313-E328.
  14. Deniskova TE, Kostyunina OV, Selionova MI, Petrov SN, Kharzinova VR, Brem GG, Zinoveva NA. Assessment of genetic susceptibility to classical and atypical scrapie in five Russian locally derived sheep breeds. Journal of Animal Science. 2018;96(S3):468. doi: 10.1093/jas/sky404.1022
  15. DeVuyst EA, Bauer ML, Cheng FC, Mitchell J, Larson D. The impact of a leptin gene SNP on beef calf weaning weights. Animal Genetics. 2008;39(3): 284-286. doi: 10.1111/j.1365-2052.2008.01730.x
  16. Selionova MI, Dubovskova MP, Chizhova LN, Mikhailenko AK, Surzhikova ES, Plakhtyukova VR. Fatty acid composition of blood lipids of young beef cattle of different genotypes of CAPN1, GH, TG5,   LEP  genes.   IOP   Conf.  Series:  Earth  and  Environmental  Science. 2019;341:012079. doi: 10.1088/1755-1315/341/1/012079
  17. Hale DS, Goodson K, Savell JW. USDA Beef Quality and Yield Grades. [Internet]. Department of Animal Science, Texas A&M AgriLife Extension Service College Station, 2013. Available from: https://meat.tamu.edu/beefgrading/ (accessed 15 september 2020)
  18. Houseknecht KL, Baile CA, Matteri RL, Spurlock ME. The biology of leptin: A review. Journal of Animal Science. 1998;76(5):1405-1420. doi: 10.2527/1998.7651405x
  19. MacNeil MD, Grosz MD. Genome-wide scans for QTL affecting carcass traits in Hereford × Composite double backcross populations. Journal of Animal Science. 2002;80(9):2316-2324.
  20. Nkrumah JD, Li C, Yu J, Hansen C, Keisler DH, Moore SS. Polymorphism in the bovine leptin gene promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit. Journal of Animal Science. 2005;83(1):20-28.doi: 10.2527/2005.83120x
  21. Rocha JL, Baker JF, Womack JE, Sanders JO, Taylor JF. Statistical associations between restriction fragment length polymorphisms and Quantitative traits in beef cattle. Journal of Animal Science. 1992;70(11):3360-3370. doi: 10.2527/1992.70113360x
  22. Schenkel FS, Miller SP, Ye X, Moore SS, Nkrumah JD, Li C, Yu J, Mandell IB, Wilton JW, Williams JL. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of  beef  cattle.  Journal  of  Animal  Science. 2005;83(9):2009-2020. doi: 10.2527/2005.8392009x
  23. Stone RT, Kappes SM, Beattie CW. The bovine homologue of the obese gene maps to chromosome 4. Mamm Genome. 1996;7(5):399-400. doi: 10.1007/s003359900119
  24. Tyulebaev SD, Kadysheva MD, Litovchenko VG, Kosilov VI, Gabidulin VM. The use of single-nucleotide polymorphism in creating a crossline of meat simmentals. IOP Conf. Series: Earth and Environmental Science. 2019;341:012188. doi: 10.1088/1755-1315/341/1/012188
  25. United States Standards for Grades of Carcass Beef. United States Department of Agriculture; 2017:16 p.
  26. Zhao Q, Davis ME, Hines HC. Associations of polymorphisms in the Pit-1 gene with growth and carcass traits   in   Angus   beef   cattle.   Journal   of    Animal  Science.  2004;82(8):2229-2233. doi: 10.2527/2004.8282229x

Gerasimov Nikolay Pavlovich, Cand. Sci. (Agr.), Senior Researcher, Beef Cattle Breeding Department, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., cell: 89123589617, e-mail: nick.gerasimov@rambler.ru

Kolpakov Vladimir Ivanovich, Cand. Sci (Agr.), Researcher at the Beef Cattle Breeding Laboratory, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., tel.: 8(3532)30-81-74; e-mail: vkolpakov056@yandex.ru

Dzhulamanov Kinispay Murzagulovich, Dr. Sci. (Agr.), Head of the Laboratory for Beef Cattle Breeding, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St., cell: 8-987-840-49-28, e-mail: kinispai.d@yandex.ru

Lapshina Alexandra Andreevna, postgraduate student, breeding and genetic centre for beef breeds of cattle, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29 9 Yanvarya St.

Received: 11 December 2020; Accepted: 14 December 2020; Published: 31 December 2020

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