Elena V Sheyda, Svyatoslav V Lebedev, Sergey A Miroshnikov, Victoria V Grechkina, Vitaly A Ryazanov, Oksana V Shoshina

DOI: 10.33284/2658-3135-103-4-26

UDC 636.5:636.085.57

Acknowledgements:

The research was carried out with the support of the Russian Science Foundation. FSBSI FRC BST RAS (No. 20-16-00088)

Changes in the activity of digestive enzymes of pancreatic juice under the influence of ultrafine

particles of Cr2O3 against the background of feeding with protein diets raising cattle

Elena V Sheyda1,2, Svyatoslav V Lebedev2, Sergey A Miroshnikov2, Victoria V Grechkina2,3,

Vitaly A Ryazanov2, Oksana V Shoshina2

1Orenburg State University (Orenburg, Russia)

2 Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences (Orenburg, Russia)

3Orenburg State Agrarian University (Orenburg, Russia)

Summary. Due to a number of advantages, preparations of ultrafine particles (UFP) as sources of trace elements have been increasingly used for animals in recent years in practical animal husbandry. These drugs include chromium UFPs and used to reduce fat deposition and optimize metabolism in industrial poultry and pig farming. The aim of the present study is to study the effect of Cr2O3 UFPs on the activity of digestive enzymes in pancreatic juice against the background of feeding with diets with different protein content. The research was carried out on 8-9-month Kazakh white-headed calves with a live weight of 200-220 kg. The animals of the experimental groups were additionally injected with protein supplements - sunflower cake and soybean meal. Cr2O3 UFPs were used in the experiment: calves from groups II and IV were included UFPs at a dose of 200 mg/bird/day. Based on the data obtained, it was found that the inclusion of protein components in the diets of young animals, along with a positive effect, has a negative effect on the exocrine function of the pancreas, reducing the activity of pancreatic secretion and digestive enzymes. The inclusion of Cr2O3 UFPs in protein diets had a stimulating effect on the production of juice by the pancreas and increased the activity of digestive enzymes: amylase, lipase and intestinal proteases, which makes it possible to count on an increase in the productive qualities of cattle.

Key words: cattle, feeding, ultrafine particles, chromium, pancreas, enzymes, pancreatic juice, chyme.

  1. Balabanov VI. Nanotechnology. Science of the future. Moscow: Eksmo; 2009:247 p.
  2. Fedayev AN et al. Biological substantiation of the need for young cattle in chromium and its practical significance in the herbal type of feeding (Conference proceedings) Problems of biodiversity conservation in the North-Western Caspian region: materials of the international. scientific-practical Conf., (Elista, October 20-21, 2006). Elista: KSU im. Gorodovikovа BB; 2007:169-177.
  3. Lebedev SV, Kvan OV, Gubaidullina IZ, Gavrish IA, Grechkina VV, Momchilovich B, Ryabov NI. Effect of chromium nanoparticles on digestive enzymesactivity and morphological and biochemical parameters of calf blood. Animal Husbandry and Fodder Production. 2018;101(4):136-142.
  4. Kokorev VA. Influence of chromium on milk production of cows. Zootechniya. 2008а; 9:11-13.
  5. Kokorev VA, Fedaev AN, Gibalkina NI. Rationale for the use of chromium in feeding cattle (Conference proceedings) Actual problems of intensive development of animal husbandry: materials of the XI Intern. scientific and practical conference dedicated. To the 75th anniversary of the Department of Breeding and Genetics of Agricultural Animals of the EE "BGSKhA". Gorki: Belarusian State Agricultural Academy; 2008b:92-98
  6. Kalashnikov AP, et al. Standards and diets of farm animals: Ref. book. 3rd ed., rework. and add. Moscow: Agropromizdat; 2003:456 p.
  7. Kozinets AI, Kozinets TG, Golushko OG, Nadarinskaya MA, Grin' MS, Soloviev AV. Application of chromium nanoparticles in the diets of young cattle (Conference proceedings) Scientific support of animal husbandry in Siberia: materials of the IV Intern. scientific-practical conf. (Krasnoyarsk, May 14-15, 2020). comp. Efimova LV, Lyubimova YuG; KrasNIIZh FRC KSC SB RAS. Krasnoyarsk; 2020:257-260.
  8. Sineschekov AD. Nutritional processes and their nervous regulation in farm animals. Abstracts of the VIII All-Union Congress of Physiologists, Biochemists, Pharmacologists. 1955:736 p.
  9. Lebedev SV, Sheida EV, Gubaidullina IZ, Gavrish IA, Kvan OV, Miroshnikov IS, Ryazanov VA, Bykov AV, Rogachev BG. Method for preparation of fodder additive for young cattle: pat. 2711259 Russian Federation. Applied. 29.03.19; publ.. 15.01.2020, Byul. № 2.
  10. Tretyakov YuD. Nanotechnology. ABC for everyone. Moscow: Fizmatlit; 2008:368 p.
  11. Albanese A, Tang PS, Chan WCW. The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annu Rev Biomed Eng. 2012;14:1-16. doi: 10.1146/annurev-bioeng-071811-150124
  12. Anderson RA. Stress effects on chromium nutrition of humans and farm animals. In: Biotechnology in the feed industry: Proceedings of Alltech's Tenth Annual Symposium, Lyons TP & Jacques KA, editors. University Press. Nottingham, UK; 1994:267-274.
  13. Choi SJ, Oh JM, Choy JH. Biocompatible nanoparticles intercalated with anticancer drug for target delivery: pharmacokinetic  and biodistribution study. J Nanosci Nanotechnol. 2010;10(4):2913-2916.  doi: 10.1166/jnn.2010.1415
  14. Davis CM, Vincent JB. Chromium oligopeptide activates insulin receptor tyrosine kinase activity. Biochemistry. 1997;36:4382-4385. doi: 10.1021/bi963154t
  15. Davis CM, Sumrall KH, Vincent JB. A biologically active form of chromium may activate a membrane phosphotyrosine phosphatase (PTP). Biochemistry. 1996;35(39):12963-12969. doi: 10.1021/bi960328y
  16. Gonzales-Eguia A, Fu C, Lu F, Lien T. Effects of nanocopper on copper availability and nutrients digestibility, growth performance and serum traits of piglets. Livest Sci. 2009;126(1-3):122-129. doi: https://doi.org/10.1016/j.livsci.2009.06.009
  17. Ichikawa S, Iwamoto S, Watanabe J. Formation of biocompatible nanoparticles by self-assembly of enzymatic hydrolysates of chitosan and carboxymethyl cellulose. Biosci Biotechnol Biochem. 2005;69(9):1637-1642.
  18. Lien TF, Horng YM, Yang KH. Performance, serum characteristics, carcase traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. Br. Poult. Sci. 1999;40(3):357-363. doi: https://doi.org/10.1080/00071669987458
  19. Mikhailova AG, Khairullin RF, Demidyuk IV, Kostrov SV, Grinberg NV, Burova TV, Grinberg VY, Rumsh LD. Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease. Protein Expres. Purificat. 2014;93:63-76. doi: https://doi.org/10.1016/j.pep.2013.10.011
  20. Mowat DN. Organic chromium. A new nutrient for stressed animals. In: In: Biotechnology in the feed industry: Proceedings of Alltech's Tenth Annual Symposium, Lyons TP & Jacques KA, editos, University Press. Nottingham, UK; 1994:275-282.
  21. Sahin K, Kucuk O, Sahin N. Effects of dietary chromium picolinate supplementation on performance, and plasma concentrations of insulin and corticostrerone in laying hens under low ambient temperature. J Anim Physiol Anim Nutr. 2001;85(5-6):142-147. doi: https://doi.org/10.1046/j.1439-0396.2001.00314.x
  22. Shim M, Kam NWS, Chen RJ, Li Y, Dai H. Functionalization of carbon nanotubes for biocompatibility and biomolecular recognition. Nano Lett. 2002;2(4):285-288. doi: https://doi.org/10.1021/nl015692j
  23. Silbergeld EK, Graham J, Price LB. Industrial food animal production, antimicrobial resistance, and  human  health.  Annu  Rev  Public  Health. 2008;29:151-169.  doi: 10.1146/annurev.publhealth.29.020907.090904
  24. Solis-Heredia MJ, Quintanilla-Vega B, Sierra-Santoyo A, Hernández J M, Brambila E, Cebrián ME, Albores A. Chromium increases pancreatic metallothionein in the rat. Toxicology. 1999;142(2):111-117. doi: 10.1016/s0300-483x(99)00130-4
  25. Taylor TM, Davidson PM. Liposomal nanocapsules in food science andagriculture. Crit Rev Food Sci Nutr. 2005;45(7-8):587-605. doi: 10.1080/10408390591001135
  26. Travan A, Pelillo C, Donati I, Marsich E, Benincasa M, Scarpa T et al. Non-cytotoxic silver nanoparticle-polysaccharide nanocomposites with antimicrobial activity. Biomacromolecules. 2009;10(6):1429-1435. doi: https://doi.org/10.1021/bm900039x
  27. Valdes MG, Gonzalez ACV, Calzon JAG, Diaz-Garcia ME. Analytical nanotechnology for food analysis. Microchim Acta. 2009;166:1-19. doi: https://doi.org/10.1007/s00604-009-0165-z
  28. Wang MQ , Wang C, Li H, Du YJ, Tao WJ, Ye SS, He YD. Effects of chromium-loaded chitosan nanoparticles on growth, blood metabolites, immune traits and tissue chromium in finishing pigs. Biol Trace Elem Res. 2012;149(2):197-203. doi: 10.1007/s12011-012-9428-3

Sheyda Elena Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.; Researcher, Experimental Biological Clinic, 460018, Orenburg, Russia, Orenburg State University, 13 Pobedy Ave, 8-922-862-64-02, e-mail: elena-shejjda@mail.ru

Lebedev Svyatoslav Valerevich, Dr. Sci. (Biol.), Leading Researcher, Laboratory for Biological Testing and Expertises, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St.,  tel.: 8-912-345-87-38, e-mail: lsv74@list.ru

Miroshnikov Sergey Aleksandrovich, Dr. Sci (Biol.), RAS Corresponding Member, Director, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-70, e-mail: fncbst@mail.ru

Grechkina Victoria Vladimirovna, Cand. Sci (Biol.), Researcher, Laboratory for Biological Testing and Expertise, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., Associate Professor, Department of Noncommunicable Animal Diseases, Orenburg State Agrarian University, 460014, Orenburg, Russia, 18 Chelyuskintsev, St.,  tel.: 8-922-877-14-97, e-mail: Viktoria1985too@mail.ru

Ryazanov Vitaly Aleksandrovich, Cand. Sci. (Agr.), Researcher of Farm Animal Feeding and Feed Technology Department named after Leushin SG., Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8(3532)30-81-79, e-mail: vita7456@yandex.ru

Shoshina Oksana Vyacheslavovna, Junior Researcher, Laboratory of Biological Testing and Expertise Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, Russia, 29, 9 Yanvarya St., tel.: 8-987-891-96-55, e-mail: oksana.shoshina.98@mail.ru

Received: 10 November 2020; Accepted: 14 December 2020; Published: 31 December 2020

Download