Marina P Dubovckova

Animal Husbandry and Fodder Production. 2022. Vol. 105, no 3. Р. 47-55.

doi:10.33284/2658-3135-105-3-47

 Original article

Genotyping of Hereford cattle for GHR, IGF-1 and GDF5 genes

 Marina P Dubovckova1

1Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1dubovskova.m@mail.ru, http://orcid.org/0000-0001-6915-4647

 Abstract. As a result of genotyping of Hereford cattle, the frequency of heterozygotes varied from 0.079±0.044 (7.90%) in GDF5 gene to 0.316±0.075 (31.6%) in IGF-I gene. Heterozygous genotypes (FY) with F allele occurred 2.3 times more than homozygous variants (FF) in GHR gene. According to the GDF5 gene, heterozygotes with T allele had a 1.5 times higher distribution frequency than homozygotes with this allele in the herd. The frequency of B allele in IGF-1 gene was 0.737 ± 0.052, there were twice as many homozygous genotypes (BB) than heterozygous B allele carriers. Progeny occupied an intermediate position between cows and sires in terms of the frequency of T and B alleles in GDF5 and IGF-1 genes. The level of observed heterozygosity in progeny was lower by 19% and 10% than in sires, and also lower by 10% and 3% than in cows, respectively for IGF-I and GDF5 genes. The indicator of the frequencies deviation of heterozygous genotypes from the theoretically expected proportion of heterozygotes was negative in all genes marker in offspring, which indicates the consolidation of young animals according to the  studied  genetic  markers.  The  effective  number  of  alleles  for  the  GHR, IGF-1 and GDF5 genes was 5-9% less in the progeny than in sires, which is explained by the specific inheritance of genetic profiles and the characteristic level of prepotency of sires.

Keywords: beef cattle breeding, Hereford breed, single nucleotide polymorphisms in GHR, IGF-I and GDF5 genes, genotype frequencies, allelic frequencies, heterozygous, homozygous

Acknowledgments: the work was performed in accordance to the plan of research works for 2021-2023 FSBRI FRC BST RAS (No. 0526-2021-0001).

For citation: Dubovckova MP. Genotyping of Hereford cattle for GHR, IGF-1 and GDF5 genes. Animal Husbandry and Fodder Production. 2022;105(3):47-55. (In Russ.). https://doi.org/10.33284/2658-3135-105-3-47

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Information about the authors:

Marina P Dubovckova, Dr. Sci. (Agriculture), Leading Researcher, Breeding and Genetic Center For Beef Cattle Breeds, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29 9 Yanvarya St., Orenburg, 460000, tel.: 8-922-621-61-78.

The article was submitted 23.08.2022; approved after reviewing 07.09.2022; accepted for publication 12.09.2022.

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