Antonina A Novikova, Olga S Grechishkina, Alexander A Zorov, Olga V Bogdanova

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 2. Р. 226-238.

 

Original article

Resistance of barley samples of the Orenburg selection to major diseases

 

Antonina A Novikova1, Olga S Grechishkina2, Alexander A Zorov3, Olga V Bogdanova4

1,2,3,4Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1tony-novikova@yandex.ru, https://orcid.org/0000-0002-6947-9262

2vip.olga1979@gmail.com, https://orcid.org/0000-0002-4054-3048

3zorov78@yandex.ru, https://orcid.org /0000-0001-9995-378Х

4olga_bogdanova_1995@mail.ru, https://orcid.org/0000-0002-6837-9905

 

Abstract. Studies to evaluate spring barley samples for the identification of genotypes carrying resistance genes to the main fungal diseases [1]of the region were carried out in the Orenburg region in 2021-2022. 60 varieties and promising lines of spring barley of Orenburg selection were analyzed using molecular markers for the presence of resistance genes to stem (Rpg1) and dwarf (Rph7) rust, dusty (Un8) and solid (Ruh) smut. DNA of the selected samples was isolated from 5-day-old seedlings for polymerase chain reaction (PCR). In a field experiment, disease resistance was evaluated in the same varieties against a natural infectious background. The weather conditions of the years of the study were favorable for the development of diseases. In total, 75% of the studied samples carried one gene of resistance to smut or rust diseases in their genotype. 52% of the studied material was among the genotypes carrying alleles of Un8 gene. 12 samples turned out to be sources of alleles of Rph7 gene. Carriers of Ruh gene accounted for only 3%. The study of the resistance of barley samples in field studies has largely confirmed the effectiveness of the use of DNA technologies in the search for donors of economically valuable traits.

Keywords: spring barley, Hordeum vulgare, PCR analysis, molecular markers, smut diseases, rust diseases, resistance, biotic stress

Acknowledgments: the work was supported by Department of Science and High Education of Russian  Federation  in  the  form  of a  subsidy  for  the creation of a breeding and seed center, project No. 075-15-2021-563.

For citation: Novikova AA, Grechishkina OS, Zorov AA, Bogdanova OV. Resistance of barley samples of the Orenburg selection to major diseases. Animal Husbandry and Fodder Production. 2023;106(2):226-238. (In Russ.). https://doi.org/10.33284/2658-3135-106-2-226

 

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Information about the authors:

Antonina A Novikova, Cand. Sci. (Agriculture), Leading Researcher, Head of the Laboratory for Breeding and Genetic Research in Plant Growing, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89228884481.

Olga S Grechishkina, Cand. Sci. (Agriculture), Researcher, Head of the Laboratory for Spring Barley Breeding, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89225314123.

Alexander A Zorov, Cand. Sci. (Agriculture), Leading Researcher, Deputy Director – Head of the Research Institute of Agricultural Sciences, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.:89228293471.

Olga V Bogdanova, Laboratory Research Assistant, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 27/1 Gagarin Ave., Orenburg, 460051, tel.: 89878716655.

 

The article was submitted 16.05.2023; approved after reviewing 30.05.2023; accepted for publication 13.06.2023.

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