Ivan F Gorlov, Marina I Slozhenkina, Elena Yu Anisimova, Ekaterina V Karpenko, Kermen Ye Badmaeva, Viktoriya S Ubushieva

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 3. Р. 21-34.

 

doi:10.33284/2658-3135-106-3-21

 

Original article

Polymorphism of the GH, MC4R and CAPN1 genes in southern beef cattle populations and their impact on live weight

 

Ivan F Gorlov1, Marina I Slozhenkina2, Elena Yu Anisimova3, Ekaterina V Karpenko4,

Kermen Ye Badmaeva5, Viktoriya S Ubushieva6

1,2,3,4,5,6Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd, Russia

7,8,9Volgograd State Technical University, Volgograd, Russia

1,7niimmp@mail.ru, https://orcid.org/0000-0002-8683-8159

2,8niimmp@mail.ru, https://orcid.org/0000-0001-9542-5893

3elanis1009@mail.ru, https://orcid.org/0000-0002-7508-3897

4,9ekatkarpenko@yandex.ru, https://orcid.org/0000-0003-3643-6431

5uni@kalmsu.ru, https://orcid.org/0000-0002-4065-6720

6vicki_93g@mail.ru, https://orcid.org/0000-0003-0320-7771

 

Abstract. Studies have been carried out to study the genetic structure of cattle populations of three breeds of beef productivity, grown in Volgograd region and the Republic of Kalmykia, based on the identification of polymorphic forms of the GH, MC4R and CAPN1 genes by the PCR-RFLP method. In total, 300 DNA samples obtained from animals of the Russian polled, Kazakh white-headed and Kalmyk breeds were analyzed. The article presents the optimal parameters of amplification and restriction reactions, as well as electrophoretic detection of restriction fragments. Based on the data obtained in the populations, the frequencies of alleles and genotypes were calculated, and the nature of the distribution of polymorphic variants of genes was described. As a result of associative analysis, a positive relationship between desirable genotypes and live weight was established. Taking into account the heterogeneity of the genetic architecture of the studied groups, the relationship of gene polymorphism with the productivity of animals in basic enterprises, it is recommended to conduct breeding selection in the direction of increasing homozygosity for the desired genotypes. Thus, according to the somatotropin gene, the VV genotype is desirable; for the melanocortin 4 receptor gene - GG; according to the calpain gene - SS. Using in breeding animals in which the desired genotypes were identified as a result of the studies performed, it is possible to improve the meat productivity of the offspring.

Keywords: beef cattle, Kazakh white-headed, Kalmyk, Russian Polled, cattle breeding, SNP, meat productivity

Acknowledgments: the work was completed in accordance to the plan of research works for 2022-2024 NIIMMP (No. 1021051101432-7).

For citation: Gorlov IF, Slozhenkina MI, Anisimova EYu, Karpenko EV, Badmaeva KYe, Ubushieva VS. Polymorphism of the GH, MC4R and CAPN1 genes in southern beef cattle populations and their impact on live weight. Animal Husbandry and Fodder Production. 2023;106(3):21-34. (In Russ.). https://doi.org/10.33284/2658-3135-106-3-21

 

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Information about the authors:

Ivan F Gorlov, Dr. Sci. (Agriculture), Professor, Academician of Russian Academy of Sciences, Scientific Director, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia; Head of the Department of Food Production Technology, Volgograd State Technical University, Volgograd region, Volgograd, avenue named after V.I. Lenin, 28, 400005, Russia, tel.: 8(844)239-10-48.

Marina I Slozhenkina, Dr. Sci. (Biology), Professor, Corresponding Member of Russian Academy of Sciences, Director, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia; Professor of the Department of Food Production Technology, Volgograd State Technical University, Volgograd region, Volgograd, avenue named after V.I. Lenin, 28, 400005, Russia, tel.: 8 (844) 239-10-48.

Elena Yu Anisimova, Cand. Sci. (Biology), Leading Researcher, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia, tel.: 8 (844) 239-10-48.

Ekaterina V Karpenko, Cand. Sci. (Biology), Leading Researcher, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia; Associate Professor of the Department of Food Production Technology, Volgograd State Technical University, Volgograd region, Volgograd, avenue named after V.I. Lenin, 28, 400005, Russia, tel.: 8(844)239-10-48.

Kermen Ye Badmaeva, Cand. Sci. (Biology), Applicant of Academic Degree, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia, tel.: 8 (844) 239-10-48.

Viktoriya S Ubushieva, Applicant of Academic Degree, Volga Region Research Institute for the Production and Processing of Meat and Dairy Products, Volgograd region, Volgograd, street named after Marshal Rokossovsky 6, 400066, Russia, tel.: 8 (844) 239-10-48.

 

The article was submitted 26.07.2023; approved after reviewing 07.08.2022; accepted for publication 11.09.2023.

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