Elena V Sheida, Svyatoslav V Lebedev

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 3. Р. 8-20.

 

doi:10.33284/2658-3135-106-3-8

 

Original article

Influence of Cr2O3 UFP on fermentation processes in rumen of ruminants in in vitro experiments

 

Elena V Sheida1,3, Svyatoslav V Lebedev2

1,2Federal Research Centre for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

3Orenburg State University, Orenburg, Russia

1,3elena-snejjda@mail.ru, https://orcid.org/0000-0002-2586-613X

2lsv74@list.ru, https://orcid.org/0000-0001-9485-7010

 

Abstract. With the increase in livestock worldwide, the use of metabolic modifiers has become an important area for many researchers looking for a supraphysiological diet to improve production parameters. To improve the productivity of highly productive cows, an optimal balance of all nutrients, including minerals, is important. Chromium is one of the important trace elements that plays an important role in the metabolism of ruminants, but the mechanism of its action and its effect on metabolic processes in the body are not fully understood, and sometimes contradictory. The aim of our study is to study the additional inclusion of ultrafine particles (UFP) of Cr2O3 on the fermentation processes in the rumen of ruminants by in vitro method. Experimental studies have shown that the additional introduction of Cr2O3 UFP did not change the structure of the bacterial community, but contributed to an increase in the number of representatives of the Bacteroidetes phylum by 9.2% responsible for the digestion of protein foods, and also stimulated the growth of representatives of the families Ruminococcaceae, Selenomonadaceae, Prevotellaceae, Lentimicrobiaceae by 9,1 % , 9,4 % , 20,8 %, 1,2 %. A positive correlation was found between the species composition of Olsenella microorganisms and acetic acid (r=0.72) and total nitrogen (r=0.52), Ruminococcus and propionic (r=0.73), Methanomassiliicoccus and valerian (r=0.72), Fournierella and caprona (r=0.59). An increase in the activity of the scar microbiota contributed to an increase in the digestibility of the experimental samples by 1.9% (P≤0.05) and fermentation processes, so in the scar content there was an increase in the total level of  VFA by 70.7% (P≤0.05) and total and protein nitrogen by 1.9% and 5.2%.

Keywords: ultrafine particles, chromium, digestibility, volatile fatty acids, nitrogen metabolites, microbiome

Acknowledgments: the   work   was  supported  by  the  Russian  Science  Foundation,  Project No. 23-16-00061.

For citation: Sheida EV, Lebedev SV. Influence of Cr2O3 UFP on fermentation processes in rumen of ruminants in in vitro experiments. Animal Husbandry and Fodder Production. 2023;106(3):8-20. (In Russ.). https://doi.org/10.33284/2658-3135-106-3-8

 

References

 
  1. Veselova TA, Maltseva AA, Shvets IM. Bioethical problems in biological and ecological research: an educational and methodological manual in electronic form. Nizhny Novgorod: Nizhny Novgorod State University; 2018:187 p.
  2. Georgievsky VI, Annenkov BN, Samokhin VT. Mineral nutrition of animals. Moscow: Kolos; 1979:471 p.
  3. Sheida EV, Lebedev SV, Miroshnikov SA, Grechkina VV, Ryazanov VA, Shoshina OV. Changes in the activity of digestive enzymes of pancreatic juice under the influence of ultrafine particles of Cr2O3 against the background of feeding with protein diets raising cattle. Animal Husbandry and Fodder Production. 2020a;103(4):26-36. (In Russ.)]. doi: 10.33284/2658-3135-103-4-26
  4. Kokorev V.A., Guryanov A.M., Gibalkina N.I. The exchange of chromium in the body of young cattle with haylage type of feeding. Actual problems of intensive development of animal husbandry: Collection of scientific works. Gorki: BSHA; 2017;20(1):270-283.
  5. Moskalev YuI. Mineral exchange. Moscow: Medicine; 1985:288 p.
  6. Kalashnikov AP, Fisinin VI, Shcheglov VV, Kleymenov NI. Norms and diets for feeding farm animals: Ref. book. 3rd ed., add. and reworked. Moscow; 2003:456 p.
  7. Sheida EV, Lebedev SV, Miroshnikov SA, Grechkina VV, Ryazanov VA. Assessment of influence of ultrafine particles of Cr2O3 on metabolic processes in the body of calves raised on protein diets. Animal Husbandry and Fodder Production. 2020b;103(4):14-25. doi: 10.33284/2658-3135-103-4-14
  8. Sarymsakova BE, Rozenson RI, Battakova JE.Guidelines for the ethics of scientific research: methodological recommendations. Astana; 2007:98 p.
  9. Sheida EV. Study of  the  effect  of  various additives on enzymatic processes in the rumen and the taxonomic composition of the microbiome. Agrarian Bulletin of the Urals. 2022;03(218):72-82. doi: 10.32417/1997-4868-2022-218-03-72-82
  10. Bernhard BC, Burdick NC, Rathmann RJ, Carroll JA, Finck DN, Jennings MA, Young TR, Johnson BJ. Chromium supplementation alters both glucose and lipid metabolism in feedlot cattle during the receiving period. J Anim Sci. 2012а;90(13):4857-4865. https://doi.org/10.2527/jas.2011-4982
  11. Bernhard BC, Burdick NC, Rounds W, Rathmann RJ, Carroll JA, Finck DN, Jennings MA, Young TR, Johnson BJ. Chromium supplementation alters the performance and health of feedlot cattle during the receiving period and enhances their metabolic response to a lipopolysaccharide challenge. J Anim Sci. 2012b;90(11):3879-3888. https://doi.org/10.2527/jas.2011-4981
  12. Bohrer BM, Edenburn BM, Boler DD, Dilger AC, Felix TL. Effect of feeding ractopamine hydrochloride (Optaflexx) with or without supplemental zinc and chromium propionate on growth performance, carcass characteristics, and meat quality of finishing steers. J Anim Sci. 2014;92(9):3988-3996. https://doi.org/10.2527/jas.2014-7824
  13. Byrne L, Murphy RA. Relative bioavailability of trace minerals in production animal nutrition: a Review. Animals (Basel). 2022;12(15):1981. doi: 10.3390/ani12151981
  14. Chen H, Liang P, Hu B, Zhao L, Sun D, Wang X. The application of inductively coupled plasma atomic emission spectrometry/mass spectrometry in the trace elements and speciation analysis of traditional Chinese Medicine. Spectroscopy and Spectral Analysis. 2002;22(6):1019-1024.
  15. Gaebel G, Martens H, Suendermann M, Galfi P. The effect of diet, intraruminal pH and osmolarity on sodium, chloride and magnesium absorption from the temporarily isolated and washed reticulo-rumen of sheep. Q J Exp Physiol. 1987;72(4):501-511. doi: 10.1113/expphysiol.1987.sp003092
  16. Giri AK, Sahu NP, Dash G. Improvement in the growth status and carbohydrate utilization of Labeo rohita (Hamilton, 1822) fingerlings with dietary supplementation of chromium picolinate. Fish Physiol Biochem. 2021;47(2):599-616. doi: 10.1007/s10695-021-00934-9
  17. Jeejebhoy KN, Chu RC, Marliss EB, Greenberg GR, Bruce-Robertson A. Chromium deficiency, glucose intolerance and neuropathy reversed by chromium supplementation in a patient receiving long-term total parenteral nutrition. Am J Clinical Nutrition. 1977;30(4):531-538. https://doi.org/10.1093/ajcn/30.4.531
  18. Kafilzadeh F, Karami Shabankareh H, Targhibi MR. Effect of chromium supplementation on productive and reproductive performances and some metabolic parameters in late gestation and early lactation of dairy cows. Biol Trace Elem Res. 2012;149:42-49. https://doi.org/10.1007/s12011-012-9390-0
  19. Kegley EB, Spears JW, Brown TT. Effect of shipping and chromium supplementation on performance, immune response, and disease resistance of steers. J Anim Sci. 1997;75(7):1956-1964. https://doi.org/10.2527/1997.7571956x
  20. Lalhriatpuii M, Chatterjee A, Das AK, Satapathy D, Dutta TK, Patra AK. Influence of dietary supplementation of inorganic and organic chromium on body conformation, carcass traits, and nutrient composition in muscle  and  internal  organs  of  black bengal goats. Biol Trace Elem Res. 2023:13 p. doi: 10.1007/s12011-023-03811-z
  21. Lashkari S, Habibian M, Jensen SK. A review on the role of chromium supplementation in ruminant nutrition—effects on productive performance, blood metabolites, antioxidant status, and immunocompetence. Biol Trace Elem Res. 2018; 186(2):305-321. https://doi.org/10.1007/s12011-018-1310-5
  22. Maryudi, Rahayu A, Syauqi R, Islami MK. Teknologi pengolahan kandungan kromium dalam limbah penyamakan kulit menggunakan proses adsorpsi: Review. Jurnal Teknik Kimia dan Lingkungan. 2021;5(1):90-99. doi: 10.33795/jtkl.v5i1.207
  23. NRC, 2005. Mineral Tolerance of Animals: second revised edition. National Research Council of the National Academies, Washington DC: The National Academies Press; 2005:510 p. https://doi.org/10.17226/11309
  24. Pechova A, Pavlata L. Chromium as an essential nutrient: a review. Vet Med 2007;52(1):1-18. doi: 10.17221/2010-VETMED
  25. Schwarz K, Mertz W. Chromium (III) and glucose tolerance factor. Arch Biochem Biophysics. 1959;85(1):292-295. https://doi.org/10.1016/0003-9861(59)90479-5
  26. Sheida EV, Miroshnikov SA, Duskaev GK, Atlanderova KN, Grechkina VV. Strategies for reducing ruminant methane emissions. BIO Web of Conferences: International Scientific and Practical Conference “Sustainable Development of Traditional and Organic Agriculture in the Concept of Green Economy” (SDGE 2021). 2022;42:01014. https://doi.org/10.1051/bioconf/20224201014
  27. Singh V, Singh N, Verma M, Kamal R, Tiwari R, Sanjay Chivate M, Rai SN, Kumar A, Singh A, Singh MP, Vamanu E, Mishra V. Hexavalent-chromium-induced oxidative stress and the protective role of antioxidants against cellular toxicity. Antioxidants (Basel). 2022;11(12):2375. doi: 10.3390/antiox11122375
  28. Spears JW. Boron, chromium, manganese, and nickel in agricultural animal production. Biological Trace Element Research. 2019;188(1):35-44. doi: 10.1007/s12011-018-1529-1
  29. Trojan SJ, Hergenreder JE, Canterbury LG, Leonhard JT, Clark WD, Beckett JL, Long JM. The effects of chromium propionate supplementation to yearling steers in a commercial feedyard on growth performance, carcass characteristics, and health. Transl Anim Sci. 2023;7(1):txad078. doi: 10.1093/tas/txad078
  30. Vargas-Rodriguez CF, Yuan K, Titgemeyer EC, Mamedova LK, Griswold KE, Bradford BJ. Effects of supplemental chromium propionate and rumen-protected amino acids on productivity, diet digestibility, and energy balance of peak-lactation dairy cattle. J Dairy Sci. 2014;97(6):3815-3821. https://doi.org/10.3168/jds.2013-7767
 

Information about the authors:

Elena V Sheida, Cand. Sci. (Biology), Researcher at the Laboratory of Biological Tests and Examinations, Federal Research Centre for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 460000, Orenburg, January 9, 29; Senior Researcher at the Institute of Bioelementology, Orenburg State University, pr. Pobedy, 13, Orenburg, 460018, tel: 8-922-862-64-02.

Svyatoslav V Lebedev, Dr. Sci. (Biology), Corresponding Member of Russian Academy of Sciences, Leading Researcher, Biological Tests and Examinations, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences; 29, 9 Yanvarya St., Orenburg, 460000, tel.: 8(3532)30-81-70.

 

The article was submitted 22.08.2023; approved after reviewing 28.08.2023; accepted for publication 11.09.2023.

Download