Inna P Novgorodova, Bailar S Iolchiev, Yuri A Prytkov

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 3. P. 170-178.

 

doi:10.33284/2658-3135-106-3-170

 

Original article

Changes in the parameters of nucleolar organizers in sheep under the influence of endogenous factors

 

Inna P Novgorodova1, Bailar S Iolchiev2, Yuri A Prytkov3

1,2,3Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst,

Dubrovitsy, Moscow Region, Russia

1novg-inna2005@yandex.ru, https://orcid.org/0000-0002-4617-1644

2baylar1@yandex.ru, https://orcid.org/0000-0001-5386-7263

3prytkov_y@mail.ru, https://orcid.org/0000-0003-0843-1297

 

Abstract. The activity of nucleolar organizers (NORs) depends on numerous factors that change their parameters. This property is directly related to the morphofunctional state of cells. To study the influence of endogenous factors on the parameters of the nucleoli, studies were carried out on animals kept in different conditions and different physiological states, they were divided into 4 groups (n = 5 animals in each group). The studies were carried out in the cell-engineering laboratory of the Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst. Blood samples were taken from the studied animals and blood smears were prepared for further study of the proliferative activity of cells using the silver nitrate staining technique. The following indicators were studied: the number of argyrophilic zones (AgNOR), their average color density (DNOR), the average density of NOR-free areas (DF), the total core area (SN) and the average brightness of argyrophilic zones (F). In the course of the research, it was found that in the group of animals that were classified as conditionally healthy, in a comparative analysis with the control, the following indicators were maximum: total nuclear area (SN) and average color density of argyrophilic zones (DNOR). The maximum indicators of the amount of AgNOR in the cells of animals of groups 3 and 4 (conditionally sick and dead 7-10 days after blood sampling was 5 with a frequency of 4.5%, in the control group this indicator had a high variability and the maximum number of nucleoli was 11. Thus, endogenous factors affect the parameters of the nucleoli of lymphocytes.

Keywords: nucleolar organizers (NOR), argyrophilic structure, animals, sheep, nucleolus, lymphocytes

Acknowledgments: the work was performed in accordance to the plan of research works for 2023-2024 Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst No. 0445-2021-0005.

For citation: Novgorodova IP, Iolchiev BS, Prytkov YuA. Changes in the parameters of nucleolar organizers in sheep under the influence of endogenous factors. Animal Husbandry and Fodder Production. 2023;106(3):170-178. (In Russ.). https://doi.org/10.33284/2658-3135-106-3-170

 

References

 
  1. Bugorkova SA, Shchukovskaya TN, Kurylina AF. The nucleolar apparatus of lymphocytes as an indicator of the functional activity of lymphoid organs in the preclinical evaluation of vaccines. Problems of Particularly Dangerous Infections. 2015;(2):75-78. https://doi.org/10.21055/0370-1069-2015-2-75-78
  2. Buteeva SK. Influence of the gene pool of pigs on the activity and polymorphism of interphase nucleolar organizers of lymphocytes. Bulletin of NSAU (Novosibirsk State Agrarian University). 2014;3(32):62-66.
  3. Kalaeva E, Kalaev V, Efimova K, Kaverin N, Chernickiy A. Dynamics of protein metabolism indicators and activity of lymphocyte nucleoli activity in the first month of life in calves in normal conditions and in  the  progression of bronchopneumonia. Genetics and Breeding of Animals. 2019;1:34-42. doi: 10.31043/2410-2733-2019-1-34-42.
  4. Klenovicky PM, Iolchiev BS, Bagirov VA. Analysis of the parameters characterizing the nucleolar organizers in intact lymphocytes in crossbred goats. Bulletin of the Mari State University. Series “Agricultural sciences. Economic Sciences. Economics”. 2019;5(3):298-304. doi: 10.30914/2411-9687-2019-5-3-298-304
  5. Novgorodova IP, Klenovickiy PM, Iolchiev BS. Activity link of nucleolar organaizers with proliferation level and protein biosynthesis (survey). Vestnik of Ulyanovsk State Agricultural Academy. 2020;3(51):125-135. doi: 10.18286/1816-4501-2020-3-125-135
  6. Novgorodova IP. Prospects for the use of modern methods of staining nucleolar organizers regions of cells for the diagnosis of diseases in animals. Agrarian Science. 2022;360(6):20-26. https://doi.org/10.32634/0869-8155-2022-360-6-20-26
  7. Badenhorst D, Stanyon R, Engstrom T, Valenzuela N. A ZZ/ZW microchromosome system in the spiny softshell turtle, Apalone spinifera, reveals an intriguing sex chromosome conservation in Trionychidae. Chromosome Research. 2013;21:137-147. doi: 10.1007/s10577-013-9343-2
  8. Barbosa MO, da Silva RR, de Sena Correia VC, dos Santos LP, Garnero ADV, Gunski RJ. Nucleolar organizer regions in Sittasomus griseicapillus and Lepidocolaptes angustirostris (Aves, Dendrocolaptidae): evidence of a chromosome inversion. Genet Mol Biol. 2013;36(1):70-73. doi: 10.1590/S1415-47572013000100010
  9. Britton-Davidian J, Cazaux B, Catalan J Chromosomal dynamics of nucleolar organizer regions (NORs) in the house mouse: micro-evolutionary insights. Heredity. 2012;108:68-74. doi: 10.1038/hdy.2011.105
  10. Caudron-Herger M, Pankert T, Seiler J, Németh A, Voit R, Grummt I, Rippe K. Alu element-containing RNAs maintain nucleolar structure and function. The EMBO Journal. 2015;34(22): 2758-2774. doi: 10.15252/embj.201591458
  11. Dobson JM. Significant advances in veterinary oncology 60 years on. Journal of Small Animal Practice. 2019;60(12):711-722. doi: 10.1111/jsap.13076
  12. Embaló B, Parize HN, Rivero ERC. Evaluation of cell proliferation in cystic lesions associated with impacted third molars. Microsc Res Tech. 2018;81(11):1241-1245. doi: 10.1002/jemt.23128
  13. Eroz R, Yilmaz S, Cucer N. Argyrophilic nucleolar organizing region associated protein synthesis in hair root cells of humans at different developmental stages and sex. Biotechnic & Histochemistry. 2013;88(5):267-271. https://doi.org/10.3109/10520295.2013.769632
  14. Farley KI, Surovtseva Y, Merkel J, Baserga SJ. Determinants of mammalian nucleolar architecture. Chromosoma. 2015;124(3):323-331. doi: 10.1007/s00412-015-0507-z
  15. Handa H, Kanamori H, Tanaka T, Murata K, Kobayashi F, Robinson FJ, Koh CS, Pozniak CJ, Sharpe AG, Paux E. Structural features of two major nucleolar organizer regions (NORs), Nor-B1 and Nor-B2 and chromosome-specific rRNA gene expression in wheat. The Plant Journal. 2018;96(6):1148-1159. doi: 10.1111/tpj.14094
  16. Howell WM, Black DA. Controlled silver-staining of nucleus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980;36(8):1014-1015. doi: 10.1007/BF01953855
  17. Lam YW, Trinkle-Mulcahy L. New insights into nucleolar structure and function. F1000Prime Rep. 2015;7:48. doi: 10.12703/P7-48
  18. Literman R, Badenhorst D, Valenzuela N. qPCR-based molecular sexing by copy number variation in rRNA genes and its utility for sex identification in soft-shell turtles. Methods in Ecology and Evolution. 2014;5(9):872-880. doi: 10.1111/2041-210X.12228
  19. Mangan H, Gailín MÓ and McStay B. Integrating the genomic architecture of human nucleolar organizer regions with the biophysical properties of nucleoli. The FEBS Journal. 2017;284(23):3977-3985. doi: 10.1111/febs.14108
  20. Montiel EE, Badenhorst D, Lee LS, Literman R, Trifonov V, Valenzuela N. Cytogenetic insights into the evolution of chromosomes and sex determination reveal striking homology of turtle sex chromosomes to amphibian autosomes. Cytogenetic and Genome Research. 2016;148(4):292-304. doi: 10.1159/000447478
  21. Pederson T. The nucleolus. Cold Spring Harb Perspect Biol. 2011;3:a000638. doi: 10.1101/cshperspect.a000638
  22. Prieto JL, McStay B. Pseudo-NORs: A novel model for studying nucleoli. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2008;1783(11):2116-2123. doi: 10.1016/j.bbamcr.2008.07.004
  23. Villegas-Mercado CE, Agredano-Moreno LT, Bermúdez M, Segura-Valdez ML, Arzate H, Del Toro-Rangel EF, Jiménez-García LF. Cementum protein 1 transfection does not lead to ultrastructural changes in nucleolar organization of human gingival fibroblasts. J Periodont Res. 2018;53(4):636-642. https://doi.org/10.1111/jre.12553
 

Information about the authors:

Inna P Novgorodova, Cand. Sci. (Biology), Senior Researcher, Laboratory of Cell Engineering, Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst, Podolsk Municipal District, Moscow Region, 142132, Dubrovitsy 60, tel.: 8(916)822-76-96.

Bailar S Iolchiev, Dr. Sci. (Biology), Leading Researcher of Cell Engineering, Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst, Podolsk Municipal District, 142132, Dubrovitsy 60, Moscow Region.

Yuri A Prytkov, Cand. Sci. (Biology), Researcher, Laboratory of Cell Engineering, Federal Research Centre for Animal Husbandry named after Academy Member L.K. Ernst, Podolsk Municipal District, 142132, Dubrovitsy 60, Moscow Region.

 

The article was submitted 31.05.2023; approved after reviewing 24.08.2023; accepted for publication 11.09.2023.

Download