Larisa N Skorykh, Angelina V Sukhoveeva, Antonina V Skokova, Sergei S Bobryshov

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 4. Р. 57-67.

doi:10.33284/2658-3135-106-4-57

Original article

Association of single nucleotide polymorphisms in GH, CAST genes with slaughter qualities

in Manych Merino sheep

 

Larisa N Skorykh1, Angelina V Sukhoveeva2, Antonina V Skokova3, Sergei S Bobryshov4

1,2,3,4North Caucasus Federal Agrarian Research Centre, Mikhailovsk, Russia

1smu.sniizhk@yandex.ru, https://orcid.org/0000-0002-6090-4453

2sukhovey1337@gmail.com, https://orcid.org/0000–0002–3815–1088

3antoninaskokova@mail.ru, https://orcid.org/0000–0002–2193–7498

4ssbob@yandex.ru, https://orcid.org/0000–0003–4608–8207

 

Abstract. In recent decades, advances in modern technologies using DNA markers have made it possible to identify regions of the genome that underlie complex traits such as the level of meat productivity. At the same time, traditional animal breeding programs based solely on information about phenotype and breeding, thanks to the inclusion of information about molecular genetic markers, will speed up selection to improve their productivity. Therefore, special attention is paid to the study of polymorphism of marker genes that control the processes of growth, muscle tissue development and energy metabolism in sheep breeding aimed at improving meat productivity. The study of polymorphism of the growth hormone (GH) and calpastatin (CAST) genes, presumably associated with traits of meat productivity in sheep is of particular relevance. The purpose of this study was to study the association of single nucleotide polymorphisms in the GH and CAST genes with the slaughter qualities of Manych Merino sheep. The study examined the meat productivity indicators of the analyzed livestock depending on the genotypes of the GH and CAST genes. Animals with GHAB and GHBB genotypes were superior to animals of the GHAA genotype in such indicators as: pre-slaughter live weight (10.2 and 7.3%), fresh carcass weight (15.8 and 9.1%), slaughter weight (15.7 and 9.0%). The results of the morphological composition of half-carcasses indicate that carriers of the GHAB and GHBB genotypes contained more muscle tissue by 18.8 and 10.8 % than animals of the GHAA genotype. In addition, sheep with the GHAB and GHBB genotypes were characterized by a higher meat coefficient of 11,1 and 6,8% than the analogues of the GHAA variant. Considering the slaughter qualities of sheep and taking into account the polymorphism of the CAST gene, it was revealed that animals with the CASTMN and CASTNN genotypes were distinguished by greater live weight before slaughter by 7.5 and 5.1%, fresh carcass weight by 10.7 and 5.5%, slaughter weight - by 10.5 and 5.5%, meat coefficient - by 10.1 and 5.6%, in comparison with individuals of the CAST MM genotype. Significant associations were revealed between the genotypes of the GH and CAST genes and the characteristics of meat productivity of Manych Merino sheep.

Keywords: sheep, Manych Merino breed, polymorphism, GH, CAST, meat productivity

For citation: Skorykh LN, Sukhoveeva AV, Skokova AV, Bobryshov SS. Association of single nucleotide polymorphisms in GH, CAST genes with slaughter qualities in Manych Merino sheep. Animal Husbandry and Fodder Production. 2023;106(4):57-67. (In Russ.). https://doi.org/10.33284/2658-3135-106-4-57

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Information about the authors:

Larisa N Skorykh, Dr. Sci. (Biology), Chief Researcher at the Department of Sheep Breeding and Goat Breeding, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, tel.: +7(8655)32-32-98.

Angelina V Sukhoveeva, Junior Researcher at the Laboratory of Genomic Breeding and Reproductive Cryobiology in Animal Husbandry, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, tel.: +7(8655)32-32-98.

Antonina V Skokova, Cand. Sci. (Biology), Senior Researcher at the Laboratory of Genomic Breeding and Reproductive Cryobiology in Animal Husbandry, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, tel.: +7(8655)32-32-98

Sergei S Bobryshov, Cand. Sci. (Agriculture), Senior Researcher at the Department of sheep breeding and goat breeding, North Caucasus Federal Agrarian Research Centre, 49 Nikonov st., Mikhailovsk, Stavropol Territory, 356241, tel.: +7(8655)32-32-98.

The article was submitted 25.09.2023; approved after reviewing 11.10.2023; accepted for publication 11.12.2023.

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