Olga S Romanenkova, Olga V Kostyunina

Animal Husbandry and Fodder Production. 2023. Vol. 106, no 4. P. 68-79.

doi:10.33284/2658-3135-106-4-68

Original article

Diagnosis of a mutation in the PHKG1 gene that determines PSE syndrome in pigs

Olga S Romanenkova1, Olga V Kostyunina2

1,2Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, Dubrovitsy, Russia

1y7tteaip@mail.ru, https://orcid.org/0000-0002-2682-6164

2kostolan@yandex.ru, https://orcid.org/0000-0001-8206-3221

 

 Abstract. The intensification of selection to increase productivity significantly affected the growth rate of pigs, but had a negative impact on meat quality. Thus, improving the quality of meat products remains an urgent problem in pig farming. The most common defect in pork is PSE. This defect manifests itself in the increased intensity of oxidative processes in meat, as a result of which meat becomes unsuitable for subsequent technological processing. Various unfavorable factors lead to the appearance of PSE pork with reduced technological characteristics. The difficulty of developing preventive measures to reduce the incidence of animals with such a defect in the herd is because, as a rule, such animals are identified after slaughter. Using molecular genetics methods, genes associated with pig productivity parameters, including the intensity of oxidative processes in pork, were identified, their chromosomal localization and nucleotide sequence were determined. One of these genetic markers is polymorphism in the PHKG1 gene. The aim of this work was to develop a test system for analyzing polymorphism in the PHKG1 gene (g.8283, C>A), which affects the quality of pork in the post-slaughter period and to study the frequency of occurrence of alleles and genotypes in pigs of various breeds. Laboratory of molecular genetics of farm animals of the Federal State Budgetary Scientific Institution VIZh named after L.K. Ernst developed a test system based on the polymerase chain reaction method with real-time detection of results. Genomic DNA was isolated using the K-Sorb-100 reagent kit. Genotypes were determined by the nature of the fluorescence curves. A sample of pigs was studied, including 100 samples of Large White pigs, 100 samples of Landrace pigs, 100 samples of Duroc pigs and 240 samples of F2 hybrids. For the first time, the polymorphism of the PHKG1 gene was studied in a sample of Russian reproduction pigs and the frequency of occurrence of the undesirable allele A was determined.

Keywords: pigs, meat quality, exudative meat, PSE syndrome, test system, single nucleotide polymorphism, gene, PHKG1

Acknowledgments: the work was performed in accordance to the plan of research works for 2021-2023 Federal Research Center for Animal Husbandry named after Academy Member LK Ernst (No. 0445-2021-0016).

For citation: Romanenkova OS, Kostyunina OV. Diagnosis of a mutation in the PHKG1 gene that determines PSE syndrome in pigs. Animal Husbandry and Fodder Production. 2023;106(4):68-79. (In Russ.). https://doi.org/10.33284/2658-3135-106-4-68

 

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Information about the authors:

Olga S Romanenkova, Cand. Sci. (Biology), researcher, Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, cell: +7(4967)651102.

Olga V Kostyunina, Dr. Sci. (Biology), Leading Researcher, Laboratory of Molecular Genetics of Farm Animals, Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, cell: +7(4967)651102.

The article was submitted 22.11.2023; approved after reviewing 05.12.2023; accepted for publication 11.12.2023.

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