Julia V Мukiy, Olga V Kostyunina

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 1. Р. 62-72.

 

doi:10.33284/2658-3135-107-1-62

 

Original article

Identification of LOF- mutations in a population of Ayrshire cattle

                                                                                           

Julia V Мukiy1, Olga V Kostyunina2

1Sankt-Petersburg State University of Veterinary Medicine, Sankt-Peterbug, Russia

2Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, Dubrovitsy, Russia

1jul.ma2015@yandex.ru, https://orcid.org/0000-0003-3371-298X

2kostolan@yandex.ru, https://orcid.org/0000-0001-8206-322

 

Abstract. Due to the intensification of dairy cattle breeding, the problem of accumulation of harmful LOF-mutations in cattle appeared, which reduced the quality of production, fertility of animals, as well as led to various anomalies. This study aimed to establish the frequency of mutant alleles accumulation in three genes UBE3B, CHRNB1, FMO3, associated with fertility (PIRM syndrome), multiple arthrogryposis (AM - Arthrogryposis Multiplex), and the appearance of a fishy odor in cow's milk, respectively. Genotyping of 135 heads of Ayrshire cattle was conducted in Leningrad region, which revealed the presence of undesirable haplotypes: AH1 in the UBE3B gene with a frequency of 17.0%, AHC in CHRNB1 with a frequency of 2.2%, in the FMO3 gene with a frequency of the homozygous genotype by mutation 1.5% and heterozygous – 3.0%. Thus, the occurrence of LOF mutations carriers in the population of Ayrshire cows was 23.7%. To eliminate harmful mutations, breeding work is required aimed at further identifying mutation carriers, primarily sires and cows, their culling and correction of the parental pairs selection.

Keywords: dairy cattle, Ayrshire breed; LOF-mutation, fertility haplotype, genes UBE3B, CHRNB1, FMO3

For citation: Мukiy JV, Kostyunina OV. Identification of LOF- mutations in a population of Ayrshire cattle. Animal Husbandry and Fodder Production. 2024;107(1):62-72. (In Russ.). https://doi.org/10.33284/2658-3135-107-1-62

 

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Information about the authors:

Julia V Мukiy, Cand. Sci. (Biology), Associate Professor of the Department of Animal Feeding and Breeding, St. Petersburg State University of Veterinary Medicine, 5 Chernigovskaya St., St. Petersburg, 196084, tel.: +7(921)4312412.

Olga V Kostyunina, Dr. Sci. (Biology), Leading Researcher, Laboratory of Molecular Genetics of Farm Animals, Laboratory of Molecular Genetics of Farm Animals, Federal Research Center for Animal Husbandry named after Academy Member LK Ernst, 60 Dubrovitsy, Podolsk district, Moscow region, 142132, tel.: + 7(4967)651102.

The article was submitted 25.01.2024; approved after reviewing 12.02.2024; accepted for publication 18.03.2024.

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