Ekaterina I Tarasova, Valentina S Polyakova, Elena A Sizova

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 3. Р. 8-24.

 

doi:10.33284/2658-3135-107-3-8

 

Original article

Association of DGAT1 gene polymorphism with the elemental composition of biosubstrates and productivity of black-and-white cows in the Leningrad, Vologda, and Orenburg Regions

 

Ekaterina I Tarasova1, Valentina S Polyakova2, Elena A Sizova3

1,2,3Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

1ekaterina45828@mail.ru, https://orcid.org/0000-0001-6325-7389

2valyapolyakova2001@mail.ru, https://orcid.org/0009-0002-2739-0208

3sizova.l78@yandex.ru, https://orcid.org/0000-0002-5125-5981

 

Abstract. In recent years, the increased interest in genetic mechanisms influencing the elemental composition of milk and blood in cows has led to the necessity for a deeper study of this topic. The aim of this research was to evaluate the impact of the K232A polymorphism of the DGAT1 gene on the elemental composition of hair, blood serum, and milk productivity in Black-and-White cows from three regions. Samples were collected from dairy cows in the Leningrad, Vologda, and Orenburg regions. The elemental composition was analyzed for 25 elements (Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, I, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Si, Sn, Hg, Sr, V, Zn) using inductively coupled plasma mass spectrometry. Genotyping was performed using high-throughput sequencing facilitated by the MiSeq sequencer (Illumina, USA). The results showed that in cows with the AA genotype from the Vologda region, the manganese content in the hair was 31.10% higher, and in the Leningrad region - 55.82% higher than in animals with the KK genotype; the iron concentration exceeded the indicators of this group by 136.17% and 178.43% (p=0.02), respectively. In the blood serum of animals with the KK genotype from Vologda region, the highest concentrations of nickel were recorded, exceeding levels in animals with the AA genotype by 60.81%, and in Leningrad region by 37.88%. Cows with the KK genotype in Vologda region showed higher milk yield, exceeding that of the AA genotype by 25.99% (p=0.03); in Leningrad region, the AA genotype showed 49.14% better results compared to KK, and in Orenburg region by 18.63% compared to AK. The conducted research confirms the significance of the K232A polymorphism of the DGAT1 gene for breeding work.

Key words: cattle, Black-and-White breed, diacylglycerol-O-acyltransferase, K232A, elemental composition, milk production

Acknowledgments: the work was performed in accordance to the plan of research works for 2022-2024 FSBRI FRC BST RAS (FNWZ-2022-0011).

For citation: Tarasova EI, Polyakova VS, Sizova EA. Association of DGAT1 gene polymorphism with the elemental composition of biosubstrates and productivity of black-and-white cows in the Leningrad, Vologda, and Orenburg Regions. Animal Husbandry and Fodder Production. 2024;107(3):8-24. (In Russ.). https://doi.org/10.33284/2658-3135-107-3-8

 

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Information about the authors:

Ekaterina I Tarasova, Junior Researcher at the Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

Valentina S Polyakova, Laboratory Researcher of the Laboratory of Molecular Genetic Research and Metallomics in Animal Husbandry, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

Elena A Sizova, Dr. Sci. (Biology), Head of the Centre for Nanotechnologies in Agriculture, Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, 29, 9 Yanvarya St., Orenburg, 460000.

 

The article was submitted 25.06.2024; approved after reviewing 12.08.2024; accepted for publication 09.09.2024.

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