Iolchiev BS, Schmidt AV, Lukonina ON, Moroz TA, Mashtaler DV, Kombarova NA, Ushakova SN, Pridanova IE.

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 4. Р. 255-265.

doi:10.33284/2658-3135-107-4-255

Original article

Chromatin dispersion of spermatozoa of stud bulls depending on age

 

Baylar  S Iolchiev1, Anna V Schmidt2, Olga N Lukonina3, Tatiana A Moroz4, Dmitry V Mashtaler5, Nina A Kombarova6, Svetlana N Ushakova7, Irina E Pridanova8

1,3,4,5,7,8All-Russian Scientific Research Institute of Breeding, Moscow region, Lesnye Polyany, Russia

2 Peoples' Friendship University of Russia named after Patrice Lumumba, Moscow, Russia

6“Head Center for the Reproduction of Farm Animals”, Bykovo settlement, Podolsk city district, Moscow region, Russia

1baylar1@yandex.ru, https://orcid.org/0000-0001-5386-7263

2 anna74@list.ru, https://orcid.org/0000-0002-8108-7149

3lukoninaon@mail.ru, https://orcid.org/0009-0004-5369-6555

4t_moroz_2013@mail.ru

5 mashtaler-1989@mail.ru

6komnina@list.ru, https://orcid.org/0000-0003-3861-4465

7 ob-vniiplem@yandex.ru

8irinapridanova@yandex.ru

 

Abstract. The article presents the results of a study of the relationship between the parameters characterizing the sperm quality of stud bulls and their age. The studied sample of stud bulls was divided into three groups depending on their age. Group I included animals aged up to 24 months, Group II — from 24 to 60 months, and Group III — over 60 months. In the first group, the average age of stud bulls was 21.8 months, in the second — 48.9 months, and in the third — 74 months. The results of multivariate analysis of variance indicate a statistically significant effect of stud bull age on the content of progressively motile spermatozoa in frozen-thawed semen (F = 4.25, P < 0.05). In adult stud bulls, 48.3 ± 1.13% of spermatozoa had rectilinear progressive motion, which is 12.74% more than in young animals. Pathologies are most often encountered in sperm flagella compared to other segments. They account for 56.46% of the total number of abnormal gametes. In bulls of the first group, the frequency of occurrence of pathologies in the structure of the head is 32.18% of all pathologies, which is more than in other age groups. The minimum value of the frequency of occurrence of abnormal spermatozoa was found in the samples of biomaterial of the second group. The maximum level of fragmentation of nDNA was found in a bull at the age of 14 months and amounted to 13.8%, the minimum - in a bull at the age of 49 months.

Keywords: stud bulls, age, spermatozoa, spermatozoa morphology, DNA dispersion, DNA fragmentation, pathology, spermatozoa segments

For citation: Iolchiev BS, Schmidt AV, Lukonina ON, Moroz TA, Mashtaler DV, Kombarova NA, Ushakova SN, Pridanova IE. Chromatin dispersion of spermatozoa of stud bulls depending on age. Animal Husbandry and Fodder Production. 2024;107(4):255-265. (In Russ.). https://doi.org/10.33284/2658-3135-107-4-255

 

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Information about the authors:

Baylar  S Iolchiev, Dr. Sci. (Biology), Leading Researcher, Laboratory of Reproduction Biology of Farm Animals, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin St., 13, 141212, tel.: +7(495) 515-95-57.

Anna V Schmidt, Cand. Sci. (Agriculture), Associate Professor of the Department of Public Health, Healthcare and Hygiene, Peoples' Friendship University of Russia named after Patrice Lumumba, Moscow, Miklukho-Maklaya St., 6, 117198, tel.: +7(495) 434-53-00.

Olga N Lukonina, Cand. Sci. (Agriculture), Acting Director, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin St., 13, 141212, tel.: +7(495) 515-95-57.

Tatiana A Moroz, Cand. Sci. (Biology), Senior Researcher, Laboratory of Reproduction Biology of Farm Animals, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin St., 13, 141212, tel.: +7(495) 515-95-57.

Dmitry V Mashtaler, Cand. Sci. (Biology), Head of the Laboratory of Reproduction Biology of Farm Animals, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin Street, 13, 141212, tel.: +7(495) 515-95-57.

Nina A Kombarova, Cand. Sci. (Biology), Chief Technologist of SC "Head Center for the Reproduction of Farm Animals", Moscow Region, Podolsk urban district, Bykovo settlement, Centralnaya street, 3, 142143, tel.: +7 (495) 109-99-93.

Svetlana N Ushakova, Cand. Sci. (Biology), Senior Researcher, Laboratory of Reproduction Biology of Farm Animals, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin St., 13, 141212, tel.: +7(495) 515-95-57.

Irina E Pridanova, Cand. Sci. (Biology), Senior Researcher, Laboratory of Reproduction Biology of Farm Animals, All-Russian Research Institute of Breeding, Moscow Region, Pushkino, Lesnye Polyany, Lenin St., 13, 141212, tel.: +7(495) 515-95-57.

 

The article was submitted 07.10.2024; approved after reviewing 28.10.2024; accepted for publication 09.09.2024.

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