Singina GN, Chinarov RYu, Shedova EN, Lukanina VA, Zhukova AS.

Animal Husbandry and Fodder Production. 2024. Vol. 107, no 4. Р. 283-294.

doi:10.33284/2658-3135-107-4-283

 

Original article

 

Results of oocyte isolation and obtaining IVP embryos from heifers of Istoben breed depending on OPU regimen

 

Galina N Singina1, Roman Yu Chinarov2, Ekaterina N Shedova3, Victoria А Lukanina4,

Anastasia S Zhukova5

1,2,3,4,5 Federal Research Center for Animal Husbandry – VIJ named after Academy Member L.K. Ernst, Moscow region, Dubrovitsy, Russia

1g_singina@mail.ru, https://orcid.org/0000-0003-0198-9757

2roman_chinarov@mail.ru, https://orcid.org/0000-0001-6511-5341

3shedvek@yandex.ru, https://orcid.org/0000-0002-9642-2384

4kristybatle@gmail.com, https://orcid.org/0000-0003-4744-7873

5anastasia.s.belyaeva@gmail.com, https://orcid.org/0000-0003-1155-014X

 

Abstract. In vitro embryo production (IVP) using oocytes obtained through transvaginal follicular aspiration (ovum pick up, OPU) is a widely recognized tool for breeding and preserving cattle breeds. The aim of this study was to assess the influence of individual donor factors on the efficiency of the OPU/IVP technique in the Istoben breed of cattle and the dependency of this influence on the OPU frequency. The procedure was performed on mature heifers (n=7) in the regimen of 2 (2W) and 1 (1W) times per week. Each donor underwent 8-9 consecutive OPU sessions for each compared frequency. The individual donor factor influenced the number of aspirated follicles and retrieved oocytes per OPU session (ranging from 4.8 to 13 follicles and 1.7 to 8.4 oocytes, respectively), as well as the ability of the retrieved germ cells to embryonic development in vitro. The maximum value of the blastocyst development share was 32%; the minimum was 5.3%. The number of blastocysts per OPU session ranged from 0.1 to 1.9 embryos. The effect of the OPU regimen within individual donors was observed on the number of aspirated follicles (in two heifers) in favor of 2W and on the quality of retrieved oocytes (in one heifer) in favor of 1W. No significant differences between the regimens were found in the number of retrieved oocytes or their ability to develop after in vitro fertilization to the blastocyst stage.

Keywords: cattle, Istoben breed, ovum pick up, oocytes, obtaining embryos in vitro

Acknowledgments: the work was performed in accordance to the plan of research works of the FGBNU FRC VIZh named after academician L.K. Ernst (No FGGN-2023-0004).

For citation: Singina GN, Chinarov RYu, Shedova EN, Lukanina VA, Zhukova AS. Results of oocyte isolation and obtaining IVP embryos from heifers of Istoben breed depending on OPU regimen. Animal Husbandry and Fodder Production. 2024;107(4):283-294. (In Russ.). https://doi.org/10.33284/2658-3135-107-4-283

 

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Information about the authors:

Galina N Singina, Cand. Sci. (Biology), Head of the Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry — All-Russian Research Institute of Animal Husbandry named after Academy Member L.K. Ernst, 60 Dubrovitsy settlement, Moscow Region, Podolsk, 142132, mobile: 8-9851434552.

Roman Yu Chinarov, Cand. Sci. (Veterinary), Senior Researcher, Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry — All-Russian Research Institute of Animal Husbandry named after Academy Member L.K. Ernst, 60 Dubrovitsy settlement, Moscow Region, Podolsk, 142132, mobile: 8-9153899065.

Ekaterina N Shedova, Researcher, Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry — All-Russian Research Institute of Animal Husbandry named after Academy Member L.K. Ernst, 60 Dubrovitsy settlement, Moscow Region, Podolsk, 142132, mobile: 8-9167117673.

Victoria A Lukanina, Junior Researcher, Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry — All-Russian Research Institute of Animal Husbandry named after Academy Member L.K. Ernst, 60 Dubrovitsy settlement, Moscow Region, Podolsk, 142132, mobile: 8-9165954695.

Anastasia S Zhukova, Cand. Sci. (Biology), Researcher, Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry — All-Russian Research Institute of Animal Husbandry named after Academy Member L.K. Ernst, 60 Dubrovitsy settlement, Moscow Region, Podolsk, 142132, mobile: 8-9165740148.

 

The article was submitted 07.11.2024; approved after reviewing 05.12.2024; accepted for publication 16.12.2024.

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