Chinarov RYu, Lukanina VA, Bogolyubova NV, Rykov RA.

Animal Husbandry and Fodder Production. 2025. Vol. 108. No. 1. Р. 21-32.

doi:10.33284/2658-3135-108-1-21

Original article

Dynamics of clinical blood parameters in donor heifers of the Kholmogor breed during consecutive sessions of transvaginal aspiration of follicles

 

Roman Yu Chinarov1, Victoria A Lukanina2, Nadezhda V Bogolyubova3, Roman A Rykov4

1,2,3,4Federal Research Center of Animal Husbandry named after academician LK Ernst, Dubrovitsy, Russia

1roman_chinarov@mail.ru, https://orcid.org/0000-0001-6511-5341

2kristybatle@gmail.com, https://orcid.org/0000-0003-4744-7873

3652202@mail.ru, https://orcid.org/0000-0002-0520-7022

4brukw@bk.ru, https://orcid.org/0000-0003-0228-8901

Abstract.  Monitoring of clinical blood parameters is of interest to identify possible early disorders of health status and optimize the use of donors for oocytes retrieval by transvaginal ultrasound-guided follicle puncture (OPU). The aim of the research was to study the effect of consecutive OPU sessions on the variability of clinical blood parameters in donor heifers of the Kholmogor breed. The research was carried out at the L.K. Ernst Federal Research Center for Animal Husbandry on heifers of the Kholmogor breed (n=7) at the age of 1.5 – 2 years. The experimental studies included three periods. In the first period, blood samples were taken on the 4th, 7th, 11th, 14th, 18th and 21st days of hormonally synchronized estrus cycle. In the second period, after repeated synchronization of the estrus cycle, the 1st series of nine consecutive OPU sessions was performed twice a week with alternating intervals of 3 and 4 days on the 4th, 7th, 11th, 14th, 18th, 21st, 25th, 28th and 32nd days the estrus cycle. In the third period, after a 1-month rest period, the 2nd series of nine consecutive OPU sessions was performed in a similar timing regimen. Blood samples were collected on the days of the OPU sessions. The values of clinical parameters were determined: the number of erythrocytes, the average volume of erythrocytes, hemoglobin content, hematocrit, the number of leukocytes and their distribution into lymphocytes, medium cells, including monocytes, basophils, eosinophils and their precursors, and granulocytes. Comparison of the values of clinical blood parameters on the days of OPU sessions with similar days of the estrus cycle, in which follicle aspiration was not performed, in most cases, did not reveal significant differences. Considering that the values of the studied clinical blood parameters were within the values of the indicators observed by a number of authors, the data obtained can be considered as an indication of the absence of a visible effect of intensive OPU sessions on the clinical status of donors. In this regard, a timing regime assuming two series of nine consecutive OPU sessions twice a week with a rest period of 1 month between series can be recommended for retrieval of oocytes from donor heifers of the Kholmogor breed.

Keywords: donor heifers, Kholmogory breed, OPU, clinical blood parameters

Acknowledgments: the   work   was  supported  by  the  Russian  Science   Foundation, project No. 24-76-10028.

For citation: Chinarov RYu, Lukanina VA, Bogolyubova NV, Rykov RA. Dynamics of clinical blood parameters in donor heifers of the Kholmogor breed during consecutive sessions of transvaginal aspiration of follicles. Animal Husbandry and Fodder Production. 2025;108(1):21-32. (In Russ.)]. https://doi.org/10.33284/2658-3135-108-1-21

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Information about the authors:

Roman Yu Chinarov, Cand. Sci. (Veterinary), Senior Researcher at the Laboratory of Experimental Embryology, Federal Research Center for Animal Husbandry – VIZ named after Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk, Moscow region, 142132, tel.: +74967651163.

Victoria A Lukanina, Cand. Sci. (Biology), Junior Researcher at the Laboratory of Experimental Embryology, Federal Research Center of Animal Husbandry – VIZ named after Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk, Moscow region, 142132, tel.: +74967651163.

Nadezhda V Bogolyubova, Dr. Sci. (Agriculture), Leading Researcher, Head of the Department of Physiology and Biochemistry of Farm Animals, Federal Research Center of Animal Husbandry – VIZ named after Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk, Moscow region, 142132, tel.: +74967651169.

Roman A Rykov, Senior Researcher of the Department of Physiology and Biochemistry of Farm Animals, Federal Research Center of Animal Husbandry – Academician L.K. Ernst, 60 Dubrovitsy village, Podolsk, Moscow region, 142132, tel.: +74967651169.

The article was submitted 21.10.2024; approved after reviewing 26.02.2025; accepted for publication 17.03.2025.

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